Effects of
            <i>Bacillus methylotrophicus</i>
            M4‐1 on physiological and biochemical traits of wheat under salinity stress

Ji, Chao; Wang, Xiaohui; Tian, Huimei; Hao, Liping; Wang, Changdong; Zhou, Yanyan; Xu, Ruiping; Song, Xin; Liu, Yue; Du, Jianfeng; Liu, Xunli

doi:10.1111/jam.14644

Cited by 16 publications

(14 citation statements)

References 97 publications

(91 reference statements)

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“…The development and utilization of salt-tolerant PGPR are feasible measures for increasing crop yield under salt stress ( Sáenz-Mata et al., 2016 ). PGPR are involved in various biological processes such as the mobilization of soluble phosphorus ( Zhu et al., 2011 ); fixation of nitrogen ( Yan et al., 2015 ); increase of antioxidant enzymes levels ( Ullah and Bano, 2015 ); regulation of ion transport protein expression ( Etesami, 2018 ); secretion of extracellular polysaccharides ( Qin et al., 2016 ); and production of plant hormones, iron carriers, and ACC deaminases ( Ji et al., 2020 ). PGPR can also alter the root structure, morphology, hydraulic conductivity, and hormonal status and can release various volatile compounds (such as glutamic acid, proline, and peptide) associated with stress accumulation infiltration, killing pathogens ( Arora et al., 2013 ; Olanrewaju et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Enterobacter cloacae HG-1 on the Nitrogen-Fixing Community Structure of Wheat Rhizosphere Soil and on Salt Tolerance

Hao

Song

et al. 2020

Front. Plant Sci.

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The present study investigated the physiological and biochemical characteristics of Enterobacter cloacae HG-1 isolated from saline-alkali soil. We further studied the effect of this strain on the salt tolerance of wheat and on the community structure of nitrogen-fixing bacteria in rhizosphere soil. We determined that the investigated strain had high nitrogen fixation activity and produced iron carriers, 1-aminocyclopropane-1-carboxylic acid deaminase, and plant hormones. The metabolites of this strain contained 2,3-butanediol, [R-(R*, R*)], 2-heptanone, and other growth-promoting and antibacterial substances. The strain was also highly salt-tolerant (10% NaCl). After the inoculation of wheat with the HG-1 strain, we recorded increases in root length, plant height, fresh weight, and dry weight of 19.15%, 18.83%, 16.67%, and 17.96%, respectively, compared with uninoculated plants ( P < 0.05). Compared with the leaves of uninoculated plants, the proline concentration in the leaves of inoculated plants increased by 12.43% ( P < 0.05), the malondialdehyde level decreased by 27.26% ( P < 0.05), K + increased by 20.69%, Ca 2+ increased by 57.53% and Na + decreased by 31.43% (all P <0.05). Furthermore, we detected that inoculation with the HG-1 strain did not affect the species composition of nitrogen-fixing bacteria in wheat rhizosphere soil at the phylum level. However, the average relative abundance of Proteobacteria was significantly increased, whereas the abundance of Verrucomiorobia was significantly decreased compared with uninoculated plants. At the genus level, we detected 32 genera in control samples and 27 genera in inoculated samples, and the species diversity and relative abundance of samples inoculated with the HG-1 strain decreased compared with uninoculated plants. Inoculated samples had lower abundances of Azospirillum , Rhodomicrobium , and Anabaena . Our study demonstrated that the inoculation of wheat with E. cloacae HG-1 could promote the growth of wheat under salt stress and increase salt stress tolerance. The results of this study investigating the interaction among soil, plants, and microorganisms supplement agricultural microbial databases and could provide a reference for the development of microbial-based saline soil improvement programs.

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Section: Introductionmentioning

confidence: 99%

Effects of Enterobacter cloacae HG-1 on the Nitrogen-Fixing Community Structure of Wheat Rhizosphere Soil and on Salt Tolerance

Hao

Song

et al. 2020

Front. Plant Sci.

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“…PGPR are effective as stress mitigators, and they show relatively better improvement in growth and yield as well as oxidation parameters of the salt-affected plants [ 40 ]. Studies have shown that inoculation with PGPR can promote the growth of wheat and maize in saline-alkali soils and reduce the damage caused by salt stress [ 17 , 41 ]. Using PGPR for biological control can replace chemical pesticides and manage plant diseases caused by various crop pathogens [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…After purifying isolates, the salt tolerance, ACC deaminase, and IAA production of the isolated strains were determined as follows. The dilution coating method was used to detect the surviving strain in Luria–Bertani (LB) broth with an NaCl concentration of 10% [ 17 ]. ACC deaminase production was quantitatively determined using the method described by Penrose and Glick [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

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The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

Zhou

Hao

et al. 2021

BioMed Research International

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As the main economic crop cultivated in the Yellow River Delta, winter jujube contains various nutrients. However, soil salinization and fungal diseases have affected the yield and quality of winter jujube. In order to use plant growth-promoting rhizobacteria (PGPR) to reduce these damages, the antagonistic bacteria CZ-6 isolated from the rhizosphere of wheat in saline soil was selected for experiment. Gene sequencing analysis identified CZ-6 as Bacillus amyloliquefaciens. In order to understand the salt tolerant and disease-resistant effects of CZ-6 strain, determination of related indicators of salt tolerance, pathogen antagonistic tests, and anti-fungal mechanism analyses was carried out. A pot experiment was conducted to evaluate the effect of CZ-6 inoculation on the rhizosphere microbial community of winter jujube. The salt tolerance test showed that CZ-6 strain can survive in a medium with a NaCl concentration of 10% and produces indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Studies on the inhibition mechanism of pathogenic fungi show that CZ-6 can secrete cellulase, protease, and xylanase. Gas chromatography-mass spectrometry (GC-MS) analysis showed that CZ-6 can release volatile organic compounds (VOCs), including 2-heptanone and 2-nonanone. In addition, the strain can colonize the rhizosphere and migrate to the roots, stems, and leaves of winter jujube, which is essential for plant growth or defense against pathogens. Illumina MiSeq sequencing data indicated that, compared to the control, the abundance of salt-tolerant bacteria Tausonia in the CZ-6 strain treatment group was significantly increased, while the richness of Chaetomium and Gibberella pathogens was significantly reduced. Our research shows that CZ-6 has the potential as a biological control agent in saline soil. Plant damage and economic losses caused by pathogenic fungi and salt stress are expected to be alleviated by the addition of salt-tolerant antagonistic bacteria.

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“…Several species of Bacillus have been shown to improve plant tolerance to salt stress, such as Bacillus megaterium in corn (Marulanda et al 2010), Bacillus spp. in corn (Vardharajula et al 2011), Bacillus amyloliquefaciens SN13 in rice (Nautiyal et al 2013;Tiwari et al 2017), B. licheniformis in wheat (Singh and Jha 2016), B. amyloliquefaciens in cotton and okra (Irizarry and White 2017), B. licheniformis SA03 in chrysanthemum (Zhou et al 2017), Bacillus pumilus S2 and Bacillus mojavensis S1 in barley (Mahmoud et al 2017) (Ji et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Multifaceted intervention of Bacillus spp. against salinity stress and Fusarium wilt in tomato

Medeiros

Bettiol²

2021

J Appl Microbiol

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Aim: This study aimed to screen halotolerant Bacillus strains able to promote growth and protect tomato plants against salt stress and Fusarium wilt (Fusarium oxysporum f. sp. lycopersici). Methods and Results: We evaluated some halotolerant strains of Bacillus spp. (Bacillus velezensis (AP-3) and Bacillus spp. (AP-6, AP-85 and AP-100)) to promote growth of tomato plants grown under salinity stress conditions and to protect them against Fusarium wilt disease. Such strains had been previously selected among 154 bacterial strains through biochemical tests (siderophores and indoleacetic acid productions, cellulase and catalase activity, nitrogen fixation and phosphate solubilization) in the presence of 100-mmol l À1 NaCl. Besides the above-mentioned strains, B. subtilis QST-713 (Serenade TM ) was also evaluated. Compared to control plants, aboveground dry weight increased in plants inoculated with AP-6, AP-85, AP-3, AP-100 and QST-713 strains developed in the absence of salt stress. The same tendency occurred for root dry weight; however, AP-3 strain was more effective, promoting an increase of 163%, when compared to control. Chlorophyll index and height increased >40 and 53%, respectively, for all Bacillus strains. Saline stress reduced plant growth regardless of the presence of Bacillus. Height, stem diameter, and aboveground and root dry weights increased in plants treated with Bacillus strains grown under saline conditions when compared to control. Bacillus velezensis AP-3 reduced the severity of Fusarium wilt in tomato by 50% when compared to control. Conclusion: Halotolerant Bacillus strains controlled tomato Fusarium wilt, increased growth as well as tolerance to salt stress. Significance and Impact of the Study: We demonstrated the efficacy of halotolerant Bacillus strains to control Fusarium wilt and improve tomato growth. We also demonstrated that these Bacillus strains protect tomato plants against salt stress. Bacillus can be used in an eco-friendly way because they are considered Generally Recognized As Safe.

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Effects of Bacillus methylotrophicus M4‐1 on physiological and biochemical traits of wheat under salinity stress

Cited by 16 publications

References 97 publications

Effects of Enterobacter cloacae HG-1 on the Nitrogen-Fixing Community Structure of Wheat Rhizosphere Soil and on Salt Tolerance

Effects of Enterobacter cloacae HG-1 on the Nitrogen-Fixing Community Structure of Wheat Rhizosphere Soil and on Salt Tolerance

The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

Multifaceted intervention of Bacillus spp. against salinity stress and Fusarium wilt in tomato

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