Effects of ANRIL variants on the risk of ischemic stroke: a meta-analysis

Abstract

Background : Several studies investigated the relationship between antisense non-coding RNA in the INK4 locus (ANRIL) variants and the risk of ischemic stroke (IS), yet whether ANRIL variants are associated with IS remain controversial. Therefore, we performed the present study to obtain a more conclusive result. Methods: Literature retrieval was conducted in PubMed, Medline and Embase. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. Results: Eighteen studies were enrolled for analyses. Pooled overall analyses showed that rs2383206 (recessive model: P=0.002, OR = 1.22, 95%CI 1.08–1.38; allele model: P=0.003, OR = 0.90, 95%CI 0.84–0.96) and rs10757274 (allele model: P=0.006, OR = 0.91, 95%CI 0.86–0.97) variants were significantly associated with an increased risk of IS. Further subgroup analyses by ethnicity revealed that rs2383206, rs10757274 and rs10757278 variants were all significantly correlated with an increased risk of IS in Asians. Additionally, rs10757278 polymorphism was also significantly correlated with an increased risk of IS in Caucasians. Conclusions: Our findings indicated that rs2383206, rs10757274 and rs10757278 variants may impact individual susceptibility to IS in Asians. Moreover, rs10757278 polymorphism may also impact individual susceptibility to IS in Caucasians.

Long noncoding RNA ANRIL knockdown attenuates neuroinflammation following ischemic stroke via suppressing the expression of NF-κB in vitro and in vivo

“…ANRIL has previously been studied as a biomarker associated with IS risk (W Zhang et al 2012). We observed that ANRIL expression was elevated in the hippocampus of MCAO/R mice, consistent with previous reports (Tan et al 2019;J Yang et al 2018). Our results revealed that ANRIL knockdown reduced OGD/R-induced injury and associated in ammation, whereas ANRIL overexpression aggravated these phenotypes.…”

“…ANRIL protects H9c2 cells against hypoxia-induced injury by targeting the miR-7-5p/SIRT1 axis (Shu et al 2020). Some prior bioinformatics studies have reported that ANRIL expression is altered in IS patients (Feng et al 2019;Tan et al 2019;J Yang et al 2018), but its mechanism role in IS requires further clari cation.…”

Comprehensive Analysis of Hub Genes Associated With Competing Endogenous RNA Networks in Stroke Using Bioinformatics Analysis

“…Some researchers have demonstrated that the abnormal expression of lncRNAs can be used as a diagnostic or prognostic biomarker for ischemic stroke. MALAT1 and ANRIL ( Yang et al, 2018 ; Tan et al, 2019 ; Fathy et al, 2021 ) were previously demonstrated to serve as protective or detrimental predicting factors for stroke under certain circumstances. However, even though these studies have investigated the relationships between stroke and lncRNAs, only one or two lncRNAs were focused on.…”

Epigenetic Regulation of the Vascular Endothelium by Angiogenic LncRNAs

“…Exons 13-19 of ANRIL overlapped with a high-risk haplotype associated with genetic predisposition to coronary artery disease (CAD) (Broadbent et al, 2008). A genetic association is also evident with ischemic stroke, aneurysms, and peripheral vascular diseases (Zeggini et al, 2007;Foroud et al, 2012;Kremer et al, 2015;Kong et al, 2016;Tan et al, 2019). ANRIL is especially enriched in vascular smooth muscle cells (VSMC) and mononuclear phagocytes within atherosclerotic plaques (The Encode Project Consortium, 2012;Zollbrecht et al, 2013;Bai et al, 2014;Nanda et al, 2016).…”

“…Through genome-wide association studies (GWAS), exons 13-19 of ANRIL comprise a disease-associated haplotype noted for a marked increased risk of coronary artery disease (CAD). Now we know that it is also associated with other cardiovascular diseases, such as ischemic peripheral vascular disease and ischemic stroke (Zeggini et al, 2007;Foroud et al, 2012;Kremer et al, 2015;Kong et al, 2016;Tan et al, 2019). Evidently, lncRNAs are biologically important and functionally diverse.…”