Effects of hypoxias and scaffold architecture on rabbit mesenchymal stem cell differentiation towards a nucleus pulposus-like phenotype

Feng, Ganjun; Jin, Xun; Hu, Jiang; Ma, Haiyun; Gupte, Melanie J.; Líu, Hao; Peter, X.

doi:10.1016/j.biomaterials.2011.07.049

Cited by 64 publications

(63 citation statements)

References 32 publications

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“…However, the same organization also indicates that the term MSCs may be used. To promote differentiation of animal or human MSCs, several methods were developed which involved growth factors from the transforming growth factor family (15)(16)(17)(18). An alternative approach is the co-culture of IVD cells with MSCs in the 'one-surface system' (also known as direct co-culture or contact system), three-dimensional systems (alginate beads) and culture of two cell lines separated by a porous membrane (also termed insert system, indirect co-culture or non-contact system) (19).…”

Section: Discussionmentioning

confidence: 99%

“…An alternative approach is the co-culture of IVD cells with MSCs in the 'one-surface system' (also known as direct co-culture or contact system), three-dimensional systems (alginate beads) and culture of two cell lines separated by a porous membrane (also termed insert system, indirect co-culture or non-contact system) (19). Injection of MSCs into the disc space was also assayed (16,20).…”

Section: Discussionmentioning

confidence: 99%

“…Informed consent from the legal guardians of each patient was obtained prior to the request to collect NPCs from the donors was made. The mean ± SD age of the patients was 16±2.3 years (range [14][15][16][17][18][19]. The study design was approved by the Ethics Committee of Poznan University of Medical Sciences (Poznan, Poland; approval no.…”

Section: Methodsmentioning

confidence: 99%

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Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Lehmann

Filipiak

Juzwa

et al. 2013

Molecular Medicine Reports

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Abstract. Degeneration of the intervertebral disc (IVD) is the main cause of age-related damage of spinal tissues. Using multipotent mesenchymal stromal cells (MSCs) regenerative medicine intends to restore the IVD components of annulus fibrosus (AF) and nucleus pulposus (NP). In the present study NP cells (NPCs) and MSCs obtained from adolescent patients suffering from scoliosis were used. IVDs and vertebrae were obtained during surgery and subsequently processed in order to establish cultures of NPCs and MSCs. The two cell types were co-cultured in 1-µm pore size insert system (indirect co-culture) or on one surface (direct co-culture). Prior to co-culture in these systems one of the cell types was stained by lipophilic fluorescent dye DiD (red). The results demonstrated that regardless of the cell type, the flow of DiD from stained to non-stained cells was more efficient in the direct co-culture in comparison with the insert system. Moreover, in the direct system the DiD flow was more efficient from MSCs towards NPCs compared with that in the opposite direction. These data indicated that the membrane interchange between the two cell types was asymmetric. To discriminate the subpopulation of cells that underwent membrane interchange, cells were double stained with DiD and DiO (green). In the first part of the experiment NPCs were stained by DiO and MSCs by DiD. In the second, NPCs were stained by DiD and MSCs by DiO. The cells were co-cultured in the direct system for 8 days and subsequently analyzed by flow cytometry and confocal microscopy.This analysis revealed that >50% of cells were stained by the DiO and DiD dyes. NPCs and MSCs formed structures similar to tunnelling nanotubes (TnT). In conclusion, the formation of TnT-like structures is able to promote, phenotypic changes during the direct co-culture of NPCs with MSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Lehmann

Filipiak

Juzwa

et al. 2013

Molecular Medicine Reports

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“…In our previous studies, the nanofibrous architecture was shown to provide physical cues to enhance mesenchymal stem cell differentiation into a nucleus pulposus phenotype. 3 The current study proved that the low oxygen tension and the 3D nanofibrous scaffolds could mimic the physiological environment existing in the intervertebral disc to synergistically regenerate nucleus pulposus tissue. Fig.…”

Section: Discussionmentioning

confidence: 55%

Hypoxia promotes nucleus pulposus phenotype in 3D scaffolds in vitro and in vivo

Feng¹,

Li²,

Hong

et al. 2014

SPI

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Object The role of oxygen in disc metabolism remains a matter of debate. Whether the effect of hypoxic priming on the nucleus pulposus phenotype can be maintained in vivo is not clear. The goal of the present study was to test the hypothesis that priming in a low oxygen tension in vitro could promote a nucleus pulposus phenotype in vivo. Methods Bovine nucleus pulposus cells were seeded in 3D scaffolds and subjected to varying oxygen tensions (2% and 20%) for 3 weeks. The constructs were then implanted subcutaneously for 8 weeks. Changes in the extracellular matrix were evaluated using quantitative real-time reverse transcriptase polymerase chain reaction, glycosaminoglycan (GAG) assay, DNA assay, collagen quantification, and histological and immunohistological analyses. Results Hypoxia resulted in greater production of sulfated glycosaminoglycan and higher levels of gene expression for collagen Type II, aggrecan, and SOX-9. Furthermore, after hypoxic priming, the subcutaneously implanted constructs maintained the nucleus pulposus phenotype, which was indicated by a significantly higher amount of glycosaminoglycan and collagen Type II. Conclusions Hypoxia enhanced the nucleus pulposus phenotype under experimental conditions both in vitro and in vivo. When used in combination with appropriate scaffold material, nucleus pulposus cells could be regenerated for tissue-engineering applications.

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“…Badylak et al, 1995;Dejardin et al, 2001;Jonsson et al, 2011;Kim et al, 2012;Kim et al, 2014b ; Ledet et al, 2009;Visage et al, 2006 Danhier et al, 2012;El-Amin et al, 2003;Endres et al, 2010;Feng et al, 2011;Kim et al, 2014a;Lai et al, 2014;Liang et al, 2013;Mizuno et al, 2004;Ruan et al, 2010 Poly-ε- Sinha et al, 2004;Silva et al, 2007;Li et al, 2005a;Lopez et al, 2010;Koepsell et al, 2011a ;Koepsell et al, 2011b;Martin et al, 2014 Martino et al, 2005). However, chitosan gels tend to be much softer and...…”

Section: Natural Solid Biomaterialsmentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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Effects of hypoxias and scaffold architecture on rabbit mesenchymal stem cell differentiation towards a nucleus pulposus-like phenotype

Cited by 64 publications

References 32 publications

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Co-culture of human nucleus pulposus cells with multipotent mesenchymal stromal cells from human bone marrow reveals formation of tunnelling nanotubes

Hypoxia promotes nucleus pulposus phenotype in 3D scaffolds in vitro and in vivo

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

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