Effects of hypoxia reoxygenation in brain slices from rats with type 1‐like diabetes mellitus

Correa, José Antonio González; Arrebola, María Monsalud; Cansino, A.; Muñoz‐Marín, J.; Ruiz‐Villafranca, D.; Guerrero, A.; Cuesta, Felipe Sánchez de la; Cruz, J. P. De La

doi:10.1002/dmrr.650

Cited by 13 publications

(13 citation statements)

References 103 publications

(95 reference statements)

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“…The four compounds showed cytoprotective effects separately in this experimental model, although HT and OLC showed greater potency than Ty and DHPG did. Using the same model, this effect has been previously described for HT [8,[15][16][17] and Ty [22,23] at the same proportions as in this study. OLC has demonstrated a neuroprotective effect on neuron-like SH-SY5Y cells induced with H 2 O 2 at proportions similar to those in our study and at similar concentrations [24].…”

Section: Discussionmentioning

confidence: 58%

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Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain

Cruz

Algaba²,

Martín-Aurioles³

et al. 2021

Brain Sciences

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Hydroxytyrosol (HT) is the component primarily responsible for the neuroprotective effect of extra virgin olive oil (EVOO). However, it is less effective on its own than the demonstrated neuroprotective effect of EVOO, and for this reason, it can be postulated that there is an interaction between several of the polyphenols of EVOO. The objective of the study was to assess the possible interaction of four EVOO polyphenols (HT, tyrosol, dihydroxyphenylglycol, and oleocanthal) in an experimental model of hypoxia-reoxygenation in rat brain slices. The lactate dehydrogenase (LDH) efflux, lipid peroxidation, and peroxynitrite production were determined as measures of cell death, oxidative stress, and nitrosative stress, respectively. First, the polyphenols were incubated with the brain slices in the same proportions that exist in EVOO, comparing their effects with those of HT. In all cases, the cytoprotective and antioxidant effects of the combination were greater than those of HT alone. Second, we calculated the concentration–effect curves for HT in the absence or presence of each polyphenol. Tyrosol did not significantly modify any of the variables inhibited by HT. Dihydroxyphenylglycol only increased the cytoprotective effect of HT at 10 µM, while it increased its antioxidant effect at 50 and 100 µM and its inhibitory effect on peroxynitrite formation at all the concentrations tested. Oleocanthal increased the cytoprotective and antioxidant effects of HT but did not modify its inhibitory effect on nitrosative stress. The results of this study show that the EVOO polyphenols DHPG and OLC increase the cytoprotective effect of HT in an experimental model of hypoxia-reoxygenation in rat brain slices, mainly due to a possibly synergistic effect on HT’s antioxidant action. These results could explain the greater neuroprotective effect of EVOO than of the polyphenols alone.

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Section: Discussionmentioning

confidence: 58%

“…We chose the in vitro hypoxia-reoxygenation model using rat brain slices because it has been shown that, in this model, there is cell death accompanied by an increase in oxidative and nitrosative stress [ 16 , 17 ]. This was fulfilled in the present study, as can be seen in Table 2 .…”

Section: Discussionmentioning

confidence: 99%

Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain

Cruz

Algaba²,

Martín-Aurioles³

et al. 2021

Brain Sciences

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“…Iadecola et al 30,31 reported that iNOS may be involved in neuronal death in the postischemic period because iNOS mRNA expression peaked at 24 hours after the ischemia in permanent ischemic brains and at 12 hours in transient ischemic brains after 2 hours of MCAO. 33,34 Overproduction of NO by nNOS and iNOS in the brain parenchyma has been demonstrated to contribute to tissue damage. 32 It was suggested that iNOS expression in the ischemic penumbra was associated with the recruitment of penumbra into infarction after reperfusion.…”

Section: Discussionmentioning

confidence: 99%

The Neuroprotective Effect of Agmatine After Focal Cerebral Ischemia in Diabetic Rats

Cui¹,

Lee²,

Kim³

et al. 2012

Journal of Neurosurgical Anesthesiology

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“…Diabetic nervous tissue damage can occur in the peripheral nerves or in the central nervous system [4,5]. A greater sensitivity of the central nervous tissue to ischemic damage, independent of diabetic vascular alterations, was previously described in models of experimental diabetes [6,7]. Likewise, neuronal damage even earlier than vascular damage was described in the retinas of diabetic animals [8,9].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio

Rodríguez-Pérez

Algaba²,

Martín-Aurioles³

et al. 2022

Nutrients

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The aim of this study was to assess the possible neuroprotective effect of 3′,4′-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia–reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.

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Effects of hypoxia reoxygenation in brain slices from rats with type 1‐like diabetes mellitus

Abstract: The biochemical pathways involved in oxidative stress and neuronal death are more sensitive to hypoxia reoxygenation in type 1-like diabetic, as compared to normal, rats.

Cited by 13 publications

References 103 publications

Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain

Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain

The Neuroprotective Effect of Agmatine After Focal Cerebral Ischemia in Diabetic Rats

Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio

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