Effects of Hypoxia on the Physiology of Zebrafish (Danio rerio): Initial Responses, Acclimation and Recovery

Feng, Jianfeng; Guo, Ying; Gao, Yongfei; Zhu, Lin

doi:10.1007/s00128-015-1668-4

Cited by 18 publications

(7 citation statements)

References 32 publications

(27 reference statements)

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“…(D) As ambient P O2 declines, Ṁ O2 first decreases with a more or less constant slope, and then transitions to a much sharper slope of declinee.g. crucian carp (Nilsson, 1992), zebrafish (Feng et al, 2016), branquinha (Johannsson et al, 2018).…”

Section: Calculation Of P Crit Usually Involves Selective Data Editingmentioning

confidence: 99%

The fallacy of thePcrit – are there more useful alternatives?

Wood

2018

Journal of Experimental Biology

101

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P critgenerally defined as the P O2 below which the animal can no longer maintain a stable rate of O 2 consumption (Ṁ O2), such that Ṁ O2 becomes dependent upon P O2provides a single number into which a vast amount of experimental effort has been invested. Here, with specific reference to water-breathers, I argue that this focus on the P crit is not useful for six reasons: (1) calculation of P crit usually involves selective data editing; (2) the value of P crit depends greatly on the way it is determined; (3) there is no good theoretical justification for the concept; (4) P crit is not the transition point from aerobic to anaerobic metabolism, and it disguises what is really going on; (5) P crit is not a reliable index of hypoxia tolerance; and (6) P crit carries minimal information content. Preferable alternatives are loss of equilibrium (LOE) tests for hypoxia tolerance, and experimental description of full Ṁ O2 versus P O2 profiles accompanied by measurements of ventilation, lactate appearance and metabolic rate by calorimetry. If the goal is to assess the ability of the animal to regulate Ṁ O2 from this profile in a mathematical fashion, promising, more informative alternatives to P crit are the regulation index and Michaelis-Menten or sigmoidal allosteric analyses.

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Section: Calculation Of P Crit Usually Involves Selective Data Editingmentioning

confidence: 99%

The fallacy of thePcrit – are there more useful alternatives?

Wood

2018

Journal of Experimental Biology

101

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“…Herein we added new studies and species not reported in the previous publication (Moreira et al, 2016 ). The 14 species added in this study are Astronotus ocellatus (Marcon, 1996 ); Bunodosoma cangicum (Abujamara et al, 2014 ); Catla catla (Singh et al, 2016 ); Colossoma macropomum (Marcon, 1996 ); Crepipatella dilatata (Cubillos et al, 2016 ); Danio rerio (Feng et al, 2016 ); Larimichthys crocea (Wang et al, 2017 ); Neohelice granulata ((Geihs et al, 2016 ); Pandalus borealis (Dupont-Prinet et al, 2013 ; Pillet et al, 2016 ); Pelteobagrus fulvidraco (Yang et al, 2014 ); Pelteobagrus vachelli (Zhang et al, 2016 ); Plectus murrayi (Adhikari et al, 2009 ); Reinhardtius hippoglossoides (Pillet et al, 2016 ); Scapharca inaequivalvis (Foschi et al, 2012 ). (C) Illustrative topological drawing of metazoan radiation for 10 animal lineages (Erwin et al, 2011 ; Erwin, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Current Trends and Research Challenges Regarding “Preparation for Oxidative Stress”

et al. 2017

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Survival under stress, such as exposure to hypoxia, anoxia, freezing, dehydration, air exposure of water breathing organisms, and estivation, is commonly associated to enhanced endogenous antioxidants, a phenomenon coined “preparation for oxidative stress” (POS). The regulation of free radical metabolism seems to be crucial under these selective pressures, since this response is widespread among animals. A hypothesis of how POS works at the molecular level was recently proposed and relies on two main processes: increased reactive species production under hypoxia, and activation of redox-sensitive transcription factors and signaling pathways, increasing the expression of antioxidants. The present paper brings together the current knowledge on POS and considers its future directions. Data indicate the presence of POS in 83 animal species (71.6% among investigated species), distributed in eight animal phyla. Three main research challenges on POS are presented: (i) to identify the molecular mechanism(s) that mediate/induce POS, (ii) to identify the evolutionary origins of POS in animals, and (iii) to determine the presence of POS in natural environments. We firstly discuss the need of evidence for increased RS production in hypoxic conditions that underlie the POS response. Secondly, we discuss the phylogenetic origins of POS back 700 million years, by identifying POS-positive responses in cnidarians. Finally, we present the first reports of the POS adaptation strategy in the wild. The investigation of these research trends and challenges may prove useful to understand the evolution of animal redox adaptations and how they adapt to increasing stressful environments on Earth.

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“…Zebrafish were Journal of Fish Health Vol. 3 (2) -Dec 2023 https://doi.org/10.29303/jfh.v3i2.3249 exposed to four different levels of tolerable DO namely, three hypoxic levels (20%-30% of O2 saturation, 40%-50% of O2 saturation, 60%-70% of O2 saturation) and one normoxic level (80% and above of O2 saturation) (Feng et al, 2015) under four different experimental setups (each setup, n=30). The DO levels were regulated using nitrogen gas (25ml gas per sec) by following the methods of Butlet et al (1994).…”

Section: Stress Exposure To Zebrafishmentioning

confidence: 99%

“…It was also analysed that the fish showed behavioural distress at lethal levels of pH (Zahangir et al, 2015). The physiology of zebrafish gets highly affected when exposed to lower than tolerable range of oxygen in both adults and embryos (Feng et al, 2015;Lin et al, 2020). Fishes like Tor putitora, bonefish and Oreochromis niloticus showed behavioural fluctuations when exposed to hypoxic conditions (Xu et al, 2006;Shultz et al, 2011;Kalkhundiya et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of oxidative stress on Zebrafish under different pH and dissolved oxygen levels using MDA as biomarker

Chowdhury,

Saikia

2023

jfh

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Oxidative stress on zebrafish (Danio rerio) has been evaluated for pH (four levels namely pH 4.5-5.5, 5.5-6.5, 7.5-8.5 and 8.5-9.5) and Dissolved Oxygen (DO 20-30%, 40-50% and 60-70%) in terms of oxygen saturation. Temporally, the exposure for pH levels were maintained from as 1h, 2h, 3h and 4h and for DO, it was (4h, 8h, 12h and 16h. The malondialdehyde (MDA) was used as biomarker of oxidative stress. It was observed that out of all tissues (skeletal muscle, liver, gill and brain) analyzed, zebrafish showed highest stress response in skeletal muscle against both environmental ambiances. In case of pH levels, significant increase in MDA level in skeletal muscle was recorded at pH 4.5-5.5, and the MDA level of skeletal muscle at 2h showed significant increase at this level of pH. Whereas, at DO 20-30% saturation level, skeletal muscle tissue significantly responded at this level of DO saturation and showed the maximum MDA level compared to the other tissues. On analysis for the duration of exposure, it was observed that skeletal muscle of zebrafish showed highest levels of MDA at DO 40-50% O2 saturation for 12h. In conclusion, it has become evident that zebrafish may undergo oxidative stress at pH 4.5-5.5 when exposed for 2hours, similarly, for DO it is 20-30% saturation for 12 hour exposure.

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Effects of Hypoxia on the Physiology of Zebrafish (Danio rerio): Initial Responses, Acclimation and Recovery

Cited by 18 publications

References 32 publications

The fallacy of thePcrit – are there more useful alternatives?

The fallacy of thePcrit – are there more useful alternatives?

Current Trends and Research Challenges Regarding “Preparation for Oxidative Stress”

Evaluation of oxidative stress on Zebrafish under different pH and dissolved oxygen levels using MDA as biomarker

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