Effects of hypoxia and reoxygenation on gill remodeling, apoptosis, and oxidative stress in hypoxia-tolerant new variety blunt snout bream (Megalobrama amblycephala)

Liang, Shuang; Su, Xiaolei; Zheng, Guo‐Dong; Zou, Shu‐Ming

doi:10.1007/s10695-022-01047-7

Cited by 16 publications

(9 citation statements)

References 60 publications

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“…Therefore, the upregulated expression of Bad may influence the apoptosis of Japanese flounder gills against hypoxic stress. Similar to our study, the expression of Bad in blunt snout bream was significantly upregulated after 4 and 7 days of hypoxia, and the apoptosis rate of gill cells was also significantly increased [ 8 ]. Especially, EPAS1 showed similar changes in gene expression that the expression level was first increased about four-fold at 6 h after hypoxia and then decreased.…”

Section: Discussionsupporting

confidence: 86%

“…Hypoxia is a serious problem in aquaculture, which affects the survival of aquatic animals. Hypoxia exposure caused apoptosis of ovarian follicle cells in Atlantic croaker [ 32 ], the central system of the sturgeon of sturgeon ( Acipenser shrenckii ) [ 33 ], skeletal muscle in Japanese flounder( Paralichthys olivaceus ) [ 27 ], and gills in blunt snout bream ( Megalobrama amblycephala ) [ 8 ]. In all these studies, hypoxia seriously affected fish survival and development and caused severe apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that the morphological structures of gills in many fish showed significant changes during hypoxia [ 6 ]. Hypoxia-induced gill remodeling may be related to changes in the expression levels of apoptosis-related genes [ 7 , 8 ]. Therefore, hypoxia can induce gill morphology changes and cell apoptosis in fish gills.…”

Section: Introductionmentioning

confidence: 99%

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Acute Hypoxia Stress-Induced Apoptosis in Gill of Japanese Flounder (Paralichthys olivaceus) by Modulating the Epas1/Bad Pathway

Liu

Yang

et al. 2022

Biology

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The physiological responses and molecular mechanisms of apoptosis in Japanese flounder under hypoxic stress remain unclear. In the present study, we performed acute hypoxia stress on Japanese flounder (2.39 ± 0.84 mg/L) and detected gills responses in histomorphology and molecular mechanisms. The results showed that the volume of the interlamellar cell mass decreased and the gill lamellae prolonged, indicating the expansion of the respiratory surface area. Additionally, the fluorescence signal of apoptosis increased under hypoxic stress. In addition, the expression of two genes (EPAS1 and Bad) related to apoptosis increased about four-fold and two-fold, respectively, at 6 hours of hypoxia. Meanwhile, the result of the dual-luciferase reporter assay showed that EPAS1 is a transcription factor, which could regulate (p < 0.05) the expression of the Bad gene, and we identified the binding site of EPAS1 was the AATGGAAAC sequence located near −766. DNA methylation assay showed that hypoxia affected the methylation status of CpG islands of EPAS1 and Bad genes. All results indicated that hypoxia could activate the EPAS1/Bad signal pathway to induce gill apoptosis of Japanese flounder. Our study provides new light on understanding the molecular mechanism of hypoxia-induced apoptosis in Japanese flounder.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Acute Hypoxia Stress-Induced Apoptosis in Gill of Japanese Flounder (Paralichthys olivaceus) by Modulating the Epas1/Bad Pathway

Liu

Yang

et al. 2022

Biology

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“…On the other hand, data on intraspecific heritable or phenotypic variation in hypoxia tolerance and hypoxia‐related transcriptional responses are fragmentary and largely limited to fish (Andersen et al, 2020; Anttila et al, 2013; Borowiec & Scott, 2020; Brennan et al, 2018; Crispo et al, 2020; Gorr et al, 2010; Regan et al, 2017; Shuang et al, 2022) and select invertebrates (Falfushynska et al, 2020; Gorr et al, 2010; Sandoval‐Castillo et al, 2018). Some of these studies report data consistent with rapid appearance of local adaptation to geographically variable hypoxia likelihood (Brennan et al, 2018; Pédron et al, 2017; Regan et al, 2017; Schulte, 2014).…”

Section: Introductionmentioning

confidence: 99%

The interplay between prior selection, mild intermittent exposure, and acute severe exposure in phenotypic and transcriptional response to hypoxia

Ekwudo

Malek

Anderson

et al. 2022

Ecology and Evolution

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Hypoxia has profound and diverse effects on aerobic organisms, disrupting oxidative phosphorylation and activating several protective pathways. Predictions have been made that exposure to mild intermittent hypoxia may be protective against more severe exposure and may extend lifespan. Here we report the lifespan effects of chronic, mild, intermittent hypoxia, and short‐term survival in acute severe hypoxia in four clones of Daphnia magna originating from either permanent or intermittent habitats. We test the hypothesis that acclimation to chronic mild intermittent hypoxia can extend lifespan through activation of antioxidant and stress‐tolerance pathways and increase survival in acute severe hypoxia through activation of oxygen transport and storage proteins and adjustment to carbohydrate metabolism. Unexpectedly, we show that chronic hypoxia extended the lifespan in the two clones originating from intermittent habitats but had the opposite effect in the two clones from permanent habitats, which also showed lower tolerance to acute hypoxia. Exposure to chronic hypoxia did not protect against acute hypoxia; to the contrary, Daphnia from the chronic hypoxia treatment had lower acute hypoxia tolerance than normoxic controls. Few transcripts changed their abundance in response to the chronic hypoxia treatment in any of the clones. After 12 h of acute hypoxia treatment, the transcriptional response was more pronounced, with numerous protein‐coding genes with functionality in oxygen transport, mitochondrial and respiratory metabolism, and gluconeogenesis, showing upregulation. While clones from intermittent habitats showed somewhat stronger differential expression in response to acute hypoxia than those from permanent habitats, contrary to predictions, there were no significant hypoxia‐by‐habitat of origin or chronic‐by‐acute treatment interactions. GO enrichment analysis revealed a possible hypoxia tolerance role by accelerating the molting cycle and regulating neuron survival through upregulation of cuticular proteins and neurotrophins, respectively.

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“…Gill remodeling increased the respiratory surface area of sh, which will help the sh to get more oxygen (O 2 ) and increase the chances of sh survival in the hypoxia conditions. Some recent studies have observed the gill remodeling in some sh species after only a few days of exposure to hypoxia (Dhillon et al, 2013;Shuang et al, 2022). These studies have shown that the remodeled gills return to their original morphology when the sh are returned to normal oxygen conditions (Wu et al, 2017).…”

Section: Gill Remodelingmentioning

confidence: 99%

Mechanisms of acclimation to chronic intermittent hypoxia in the gills of Largemouth bass (Micropterus salmoides)

Liu,

Wang,

et al. 2023

Preprint

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Anthropogenically induced hypoxia in water bodies has been a stressor for fish for many years and is expected to persist in the future. In order to investigate the acclimation response of fish gills to chronic intermittent hypoxia (CIH) stress, we conducted a study using largemouth bass (Micropterus salmoides) exposed to intermittent hypoxia (dissolved oxygen level: 2.0 mg·L− 1) for either one or three hours per day, over a period of 8 weeks. Our findings indicate that exposure to CIH induced remodeling of the gills and an increase in gill surface area. We also observed significant up-regulation of genes related to glycolysis (fba, pgam1, pepck, atp-pfk, pfk-2, g6pi, gapd-1, and pk), while genes associated with cholesterol synthesis (3β-hsd, cyp51, dsdr-x1, dsdr, and dhcr7) were down-regulated following CIH exposure. Furthermore, we observed the presence of elongated megamitochondria in mitochondria-rich cells within the gills of fish exposed to hypoxia. Additionally, numerous genes involved in calcium signaling pathways were up-regulated in the gills of largemouth bass, suggesting an enhanced sensitivity of gills to environmental cues in hypoxia conditions. However, the expression levels of certain genes related to innate and adaptive immune responses were inhibited following CIH exposure. Moreover, the number of mucous cells decreased, potentially making the gills more susceptible to pathogen infections. These findings highlight the potential vulnerability of gills to pathogenic organisms in the presence of CIH. Overall, our study contributes to a better understanding of how fish acclimate to CIH.

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Effects of hypoxia and reoxygenation on gill remodeling, apoptosis, and oxidative stress in hypoxia-tolerant new variety blunt snout bream (Megalobrama amblycephala)

Cited by 16 publications

References 60 publications

Acute Hypoxia Stress-Induced Apoptosis in Gill of Japanese Flounder (Paralichthys olivaceus) by Modulating the Epas1/Bad Pathway

Acute Hypoxia Stress-Induced Apoptosis in Gill of Japanese Flounder (Paralichthys olivaceus) by Modulating the Epas1/Bad Pathway

The interplay between prior selection, mild intermittent exposure, and acute severe exposure in phenotypic and transcriptional response to hypoxia

Mechanisms of acclimation to chronic intermittent hypoxia in the gills of Largemouth bass (Micropterus salmoides)

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