Effects of hypocretin and norepinephrine interaction in bed nucleus of the stria terminalis on arterial pressure

Ciriello, John; Caverson, Monica M.; Li, Z.

doi:10.1016/j.neuroscience.2013.09.032

Cited by 11 publications

(7 citation statements)

References 128 publications

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“…The BNST is another limbic brain region that contains AT2R-eGFP neurons and fibers/terminals and is known to participate in cardiovascular, endocrine and behavioral response to stress (Choi et al 2007; Ciriello et al 2013; Crestani et al 2013). It serves as a relay between other limbic areas of the forebrain and the hypothalamus and brainstem (Crestani et al 2013) and Ang-II has been found to impact neuronal activity within the BNST via an AT1R-dependent mechanism (Lienard et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system

et al. 2014

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Angiotensin-II acts at its type-1 receptor (AT1R) in the brain to regulate body fluid homeostasis, sympathetic outflow and blood pressure. However, the role of the angiotensin type-2 receptor (AT2R) in the neural control of these processes has received far less attention, largely because of limited ability to effectively localize these receptors at a cellular level in the brain. The present studies combine the use of a bacterial artificial chromosome transgenic AT2R-enhanced green fluorescent protein (eGFP) reporter mouse with recent advances in in situ hybridization (ISH) to circumvent this obstacle. Dual immunohistochemistry (IHC)/ ISH studies conducted in AT2R-eGFP reporter mice found that eGFP and AT2R mRNA were highly co-localized within the brain. Qualitative analysis of eGFP immunoreactivity in the brain then revealed localization to neurons within nuclei that regulate blood pressure, metabolism and fluid balance (e.g., NTS and median preoptic nucleus [MnPO]), as well as limbic and cortical areas known to impact stress responding and mood. Subsequently, dual IHC/ISH studies uncovered the phenotype of specific populations of AT2R-eGFP cells. For example, within the NTS, AT2R-eGFP neurons primarily express glutamic acid decarboxylase-1 (80.3 ± 2.8 %), while a smaller subset express vesicular glutamate transporter-2 (18.2 ± 2.9 %) or AT1R (8.7 ± 1.0 %). No co-localization was observed with tyrosine hydroxylase in the NTS. Although AT2R-eGFP neurons were not observed within the paraventricular nucleus of the hypothalamus (PVN), eGFP- immunoreactivity is localized to efferents terminating in the PVN and within GABAergic neurons surrounding this nucleus. These studies demonstrate that central AT2R are positioned to regulate blood pressure, metabolism and stress responses.

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Section: Discussionmentioning

confidence: 99%

Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system

et al. 2014

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“…Because tonic activation of GABAergic neurons in the CVLM is due to both baroreceptor-dependent and baroreceptor-independent inputs, GABA release in the RVLM maintains a reduced level of resting activity of bulbospinal RVLM neurons (36,50). It has also been suggested that the RVLM receives inhibitory input from the contralateral RVLM (30) along with other brain regions (5,9).…”

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confidence: 99%

Sedentary conditions and enhanced responses to GABA in the RVLM: role of the contralateral RVLM

Dombrowski

Mueller

2017

American Journal of Physiology-Regulatory, Integrative and Comp

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A sedentary lifestyle is a major risk factor for cardiovascular disease, and both conditions are associated with overactivity of the sympathetic nervous system. Ongoing discharge of sympathetic nerves is regulated by the rostral ventrolateral medulla (RVLM), which in turn is modulated by the primary excitatory and inhibitory neurotransmitters glutamate and γ-amino-butyric acid (GABA), respectively. We reported previously that sedentary conditions enhance GABAergic modulation of sympathoexcitation in the RVLM, despite overall increased sympathoexcitation. Thus the purpose of this study was to test the hypothesis that sedentary conditions increase responsiveness to GABA in RVLM. Male Sprague-Dawley rats performed either chronic wheeling running or remained sedentary for 12-15 wk. Animals were instrumented to perform RVLM microinjections under Inactin anesthesia while mean arterial pressure (MAP) and splanchnic sympathetic nerve activity (SSNA) were recorded. Unilateral microinjections of GABA (30 nl, 0.3-600 mM) into the RVLM produced dose-dependent decreases in MAP and SSNA; however, no group differences were observed. Inhibition of the contralateral RVLM (muscimol, 2 mM, 90 nl) caused decreases in MAP and SSNA that were not different between groups but enhanced decreases in SSNA to GABA in sedentary rats only. In sinoaortic denervated rats, GABA microinjections before or after inhibition of the contralateral RVLM caused decreases in MAP and SSNA that were not different between groups. Our results suggest that the contralateral RVLM plays an important role in buffering responses to inhibition of the ipsilateral RVLM under sedentary but not physically active conditions. Based on these studies and others, sedentary conditions appear to enhance both sympathoinhibitory and sympathoexcitatory mechanisms in the RVLM. Enhanced sympathoinhibition may act to reduce already elevated sympathetic nervous system activity following sedentary conditions.

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“…The role of the GABA nerve system is positively correlated with the damage to the CF; therefore a stronger role of the GABA nerve system will induce more severe damage to CF (35). Furthermore, the activity of the Hcrt nerve system is positively correlated with the GABA nerve system, as one regulates the other (36).…”

Section: Discussionmentioning

confidence: 99%

Effect of two GABA-ergic drugs on the cognitive functions of rapid eye movement in sleep-deprived and recovered rats

Wang

Wuyun

et al. 2016

Experimental and Therapeutic Medicine

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Rapid eye movement (REM) sleep is closely associated with nervous functions. The present study aimed to evaluate the effects of gabazine and tiagabine on the cognitive functions (CF) of REM sleep-deprived and sleep recovered rats. Rats were divided into REM sleep deprivation, blank control (CC) and environmental groups. The REM sleep deprivation group was further divided into non-operation (nonOP), sham-operated (Sham), gabazine (SR) and tiagabine groups. Each group was evaluated over five time points: Sleep deprived for 1 day (SD 1 day), SD 3 day, SD 5 day, sleep recovery 6 h (RS 6 h) and RS 12 h. A rat model of REM sleep deprivation was established by a modified multi-platform water method, with CF assessed by Morris water maze. Hypothalamic γ-aminobutyric acid (GABA) and glutamic acid contents were measured via high performance liquid chromatography. The number and morphology of hypocretin (Hcrt) neurons and Fos in the hypothalamus, and GABAARα1-induced integral optical density were detected by immunofluorescence. Compared to the CC group, the nonOP and Sham group rats CF were significantly diminished, Fos-positive and Fos-Hcrt double positive cells were significantly increased, and GABA content and GABAARα1 expression levels were significantly elevated (P<0.05). The tiagabine and CC groups exhibited similar results at three time points. The CF of rats in the SR group were diminished and the number of Fos-positive and Fos-Hcrt double positive cells were significantly increased (P<0.05) at RS 6 h and RS l2 h. GABA content and GABAARα1 expression levels were significantly increased in the SR group at all time points (P<0.05), whereas only GABAARα1 expression levels were significantly increased in the tiagabine group at SD 5 day (P<0.05). The results of the present study indicated that REM sleep deprivation diminished CF, increased the number of Hcrt neurons, GABA content and GABAARα1 expression. Furthermore, all alterations were positively correlated with deprivation time and corrected by sleep recovery, as demonstrated by single-factor multi-level variance analysis at the various time points in each group. Therefore, the Hcrt nervous system may be an eligible therapeutic target for the treatment of insomnia.

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Effects of hypocretin and norepinephrine interaction in bed nucleus of the stria terminalis on arterial pressure

Cited by 11 publications

References 128 publications

Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system

Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system

Sedentary conditions and enhanced responses to GABA in the RVLM: role of the contralateral RVLM

Effect of two GABA-ergic drugs on the cognitive functions of rapid eye movement in sleep-deprived and recovered rats

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