Effects of hypo- or hyperosmotic stress on gluconeogenesis, phosphoenolpyruvate carboxykinase activity, and gene expression in jaw muscle of the crab Chasmagnathus granulata: seasonal differences

Schein, Vanessa; Chittó, Ana Lúcia F.; Etges, Rodrigo Nestor; Kucharski, Luiz Carlos; Wormhoudt, Alain Van; Silva, Roselis S.M. da

doi:10.1016/j.jembe.2004.11.005

Cited by 19 publications

(14 citation statements)

References 22 publications

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“…de Zwaan, et al, 1976;Hummel, et al, 1989;Larade, et al, 2002), glycogen was not consumed by bivalves. hypo-osmotic conditions (Chitto, Kucharski, & da Silva, 2007;Schein, Chitto, Etges, Kucharski, van Wormhoudt, & Da Silva, 2005). The gluconeogenesis pathway in bivalves was rarely investigated ; however, research on pyruvate kinase of the gastropod mollusk (Plaxton & Storey, 1985) referred the inactivation of gluconeogenesis and anaerobiosis to the enzyme which channels phosphoenolpyruvate into energy metabolism.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

A GC-MS-based metabolomics investigation on scallop (Chlamys farreri) during semi-anhydrous living-preservation

Chen

Zhang

Xiong

et al. 2015

Innovative Food Science & Emerging Technologies

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Section: Accepted Manuscriptmentioning

confidence: 99%

A GC-MS-based metabolomics investigation on scallop (Chlamys farreri) during semi-anhydrous living-preservation

Chen

Zhang

Xiong

et al. 2015

Innovative Food Science & Emerging Technologies

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“…Proteins can contribute to the maintenance of the animal during periods of starvation, originating amino acids for the gluconeogenic pathway (Vinagre and Da Silva, 2002;Sánchez-Paz et al, 2006). The activity and expression of phosphoenolpyruvate carboxykinase, an important enzyme in this pathway, showed seasonal variations in the jaw muscle of the crab N. granulata (Schein et al, 2005). The uptake of amino acids in the gills and jaw muscle of this crab also showed seasonal variations (Valle et al, 2009).…”

Section: Seasonal Variations In the Biochemical Composition Of Ocypodmentioning

confidence: 92%

Seasonal variations in the biochemical composition and reproductive cycle of the ghost crab Ocypode quadrata (Fabricius, 1787) in Southern Brazil

et al. 2010

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The ghost crab, Ocypode quadrata, is found on sandy beaches from the United States to Southern Brazil. Because there is still little information about the metabolism and reproduction of O. quadrata on the southern coast of Brazil, the objectives of this study were to (i) evaluate the effect of seasonal variations on the carbohydrate, lipid, and protein metabolism of O. quadrata at Rondinha Beach, a beach with high anthropogenic activity, and to compare it with data from Siriú Beach, which has less human activity; and (ii) describe the effect of seasonal variations on the histological characteristics of male and female gonads, in order to assess the reproductive capacity of the crabs. The gonads of male crabs showed no significant variations in the gonadosomatic index (GSI) and glycogen levels; however, histological analysis of the testes revealed that they are mature in the summer. In females, the GSI and glycogen values are higher in summer, concomitantly with the presence of mature oocytes. These results suggest that the reproductive peak of O. quadrata occurs in summer. The seasonal analysis of the biochemical parameters, as well as comparison with Siriú Beach, demonstrated that the ghost crabs of Rondinha Beach have a different pattern of metabolism than those of Siriú. This difference may be a consequence of differences in the environmental conditions as well as in the anthropogenic pressures, such as vehicle traffic and the increase in human population at the beach in summer.

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“…The pentose phosphate pathway is involved in carbohydrate metabolism in decapods during ecdysis (McWhinnie, 1962) and phosphoenolpyruvate carboxykinase (E.C. 4.1.1.32; PEPCK) is a key enzyme for gluconeogenesis (Schein et al, 2005). Crustacean hyperglycemic hormone (CHH), insulin-like peptides and insulin-like growth factors (IGF-I and IGF-II) are the main hormone in the crustacean hemolymph for glucose regulation (Verri et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Most studies on these key factors in crustaceans have focused on gene cloning, gene function and enzyme activity (Chang et al, 2010;Hsiao et al, 2002;Jeon et al, 2012;Wiwegweaw et al, 2004). There were also some studies on mRNA expression of these genes (Martinez-Quintana et al, 2014;Schein et al, 2005), but most of them focused on carbohydrate digestion, transportation or regulation. There was little research that simultaneously explores the carbohydrate digestion, transportation and regulation.…”

Section: Introductionmentioning

confidence: 99%

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Wang¹,

Li²,

Xu³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Molecular response of carbohydrate metabolism in Litopenaeus vannamei was evaluated at transcriptional level through quantitative real-time PCR analysis. The mRNA expressions of six genes hexose-6-phosphotransferase, pyruvate kinase, phosphoenolpyruvate carboxykinase, crustacean hyperglycemic hormone, glucose transporter and insulin-like growth factors binding protein in the hepatopancreas, muscle, gill and eyestalk were analyzed in shrimp fed different levels of carbohydrate and challenged with acute salinity stress. Among these genes, the relative expression of GLUT1, CHH and IGF-BP in the muscle was lower or nearly the same as the control group regardless of the dietary carbohydrate level. The relative expressions of HK and PK were much higher in the hepatopancreas, muscle, gill and eyestalk at 12 h after salinity stress. The relative expression of PEPCK showed a down-up-down tendency and the expression was much higher in different tissues (except in eyestalk) than in the control from 12 to 24 h after stress. This study indicates that there was no significant dietary carbohydrate regulation in muscle. The glycogen and amino acid in muscle were the main source energy for osmoregulation in L. vannamei when the animals were challenged with acute salinity stress. The muscle and gills were the main osmoregulation organ in shrimp under hypo-saline stress.

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Effects of hypo- or hyperosmotic stress on gluconeogenesis, phosphoenolpyruvate carboxykinase activity, and gene expression in jaw muscle of the crab Chasmagnathus granulata: seasonal differences

Cited by 19 publications

References 22 publications

A GC-MS-based metabolomics investigation on scallop (Chlamys farreri) during semi-anhydrous living-preservation

A GC-MS-based metabolomics investigation on scallop (Chlamys farreri) during semi-anhydrous living-preservation

Seasonal variations in the biochemical composition and reproductive cycle of the ghost crab Ocypode quadrata (Fabricius, 1787) in Southern Brazil

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