Effects of hypertension and ovariectomy on rat hepatocytes. Are amlodipine and lacidipine protective? (A stereological and histological study)

Abstract

Amaç: Kalsiyum kanal blokerleri, hipertansiyon (HT) tedavisinde s›kça kullan›lmaktad›r. Menapoz ve hipertansiyonun her ikiside karaci¤er hasar› ve çeflitli dolafl›m bozukluklar› için risk faktörleridir. Çal›flmam›z›n amac› overektominin indükledi¤i postmenapozal dönem ve/veya deoksikortikosteron asetat-tuz Background/aims: Calcium channel blockers are increasingly used for the treatment of hypertension. Menopause and hypertension are both important risk factors for liver damage and several other circulatory abnormalities. The aim of this study was to determine the effects of amlodipine and lacidipine in an ovariectomy-induced postmenopausal period model and a deoxycorticosterone acetate-salt-induced hypertensive model in rats. Methods: In this study, animals were divided into six groups as follows: control (Group 1), hypertension (Group 2), ovariectomy (Group 3), ovariectomy and hypertension (Group 4), ovariectomy, hypertension and amlodipine-treated (Group 5), and ovariectomy, hypertension and lacidipine-treated (Group 6

Effects of Administration of Amlodipine and Lacidipine on Inflammation-Induced Bone Loss in the Ovariectomized Rat

“…The increased intracellular calcium deposition also increased in parallel with the level of IL-1β [71]. Halici et al and our study (unpublished data) indicate that amlodipine and lacidipine have anti-inflammatory properties [3,25,72]. The mechanism of the anti-inflammatory action of calcium channel blockers is explained in many articles [73,74].…”

“…On the 59th day after the OVX, inflammation was induced in groups 2, 4, 5, and 6 by four separate subcutaneous injections of talc (magnesium silicate, 3.2 g in total per animal) in sterile saline in the back of the animals as previously described [23,24]. Groups 5 and 7 were administered AML 5 mg/kg orally [4] and groups 6 and 8 were administered LAC 4 mg/kg orally [25] from the 60th to the 80th day. Experimental design and all animal groups are shown in Fig.…”

A stereological and histopathological study of the effects of exposure of male rat testes to mercury vapor

“…According to the Cavalieri principle, the surface area of the biological object of interest can be defi ned by a specifi c point counting grid. The point densities of the point counting grid was designed based on pilot studies in which the point density of the point counting grids were designed to hit a minimum of 1,000 points per region of interest (testis and seminiferous tubules) for each animal; this was suffi cient to obtain a signifi cant coeffi cient of error (CE) (Dursun et al 2010). After scanning the images and hitting points on them ( Fig.…”

Effect of mercury vapor inhalation on rat ovary: Stereology and histopathology

“…For this purpose, two different point‐counting test grids were utilized on the parallel sections. The point density of the point‐counting grid was designed according to pilot studies, to obtain acceptable coefficient of error (CE) . The CE and coefficient of variation were estimated according to previously published formulas .…”