Effects of hyperprolactinemia on testosterone production in rat Leydig cells

Abstract

The pathogenesis of hyperprolactinemia (hyperPRL) induced hypogonadism has been suggested to be related with a dysfunction of hypothalamus-pituitary-testis axis. While the direct inhibitory effects of prolactin (PRL) on testosterone (T) release have been demonstrated, the mechanism is still unclear. Our previous study demonstrated a diminished T release in the testicular interstitial cells (TICs) from the anterior pituitary (AP)-grafted rats as compared with the control, and the pattern was in agreement with the in vivo model. However, TICs incubation cannot totally represent the response of the Leydig cells. Therefore, a Percoll gradient purified Leydig cell model was adopted to explore the response of T release under similar challenges in this study to investigate the effects of hyperPRL on the Leydig cells per se. HyperPRL in male rats was induced by grafting rat AP under the renal capsule. The control animals were grafted with rat brain cortex tissue (CX). Six weeks after grafting, the rats were sacrificed. Either TICs or Leydig cells were isolated, respectively, for in vitro incubation and challenge. Challenge drugs included human chorionic gonadotropin (hCG, 0.05 IU/ml), steroidogenic precursors (25-OH-cholesterol, 10(-6) M; pregnenolone, 10(-6) M), forskolin (an anenylyl cyclase activator, 10(-4) M) and 8-bromo-3':5' cyclic adenosine monophosphate (cAMP) (8-Br-cAMP 10(-4) M). T released by TICs or Leydig cells was determined by radioimmunoassay. The TICs from the AP-grafted rats showed lower levels of T release than the control group while the purified Leydig cells demonstrated a reverse pattern in response to challenges of hCG, steroidogenic precursors, forskolin and 8-Br-cAMP. In hyperPRL rats, a paradoxical pattern of T release between TICs and purified Leydig cells is observed. The purified Leydig cells from AP-grafted rats demonstrated a higher level amount of T release than the control after stimulation. The phenomenon can be attributed to the change of Leydig cell sensitivity to the stimulation after the effects of chronic hyperPRL. Moreover, another possibility is the role played by other interstitial cells to modulate steroidogenesis in Leydig cells.

Relationship of the Hormone-Sensitive Lipase-Mediated Modulation of Cholesterol Metabolism in Individual Compartments of the Testis to Serum Pituitary Hormone and Testosterone Concentrations in a Seasonal Breeder, the Mink (Mustela vison)1

“…The increase in prolactin secretion in spring may cause macrophages to reduce HSL protein expression and activity in the interstitium, and this in turn may be one of the causes that would decrease testosterone synthesis by Leydig cells. The report that prolactin induces accumulation of cholesterol esters in response to LH stimulation [67] and the observation that prolactin inhibition of testosterone production involves interstitial cells other than Leydig cells in the rat [68] are in agreement with this hypothesis.…”

Effects of renovascular hypertension on reproductive function in male rats

“…Thus, the increase of Ang II in the 2K/1C rats could be, indirectly, one of the causes for the reduction of plasma testosterone in these animals. Moreover, high plasma PRL can reduce the sensitivity in Leydig cells to the stimulatory action of LH and thus reduce the secretion, but not the synthesis, of testosterone (Huang et al, 2001). Additionally, long-term hyperprolactinemia reduces LHRH release and thus decreases plasma testosterone (Nasello et al, 1997).…”

Effects of sulpiride on mRNA levels of steroid 5α‐reductase isozymes in prostate of adult rats

“…Several animal and human studies have indicated that hyperprolactinemia is related to plasma-androgen concentrations, although findings are controversial (38)(39)(40). Because both 5a-R1 and 5a-R2 genes are positively regulated by T, the most abundant androgen (41), we measured plasma T levels in controls and sulpiride-treated rats.…”