Effects of hyperoxia on local and remote microcirculatory inflammatory  response after splanchnic ischemia and reperfusion

Waisman, Dan; Brod, Vera; Wolff, Rafael; Sabo, Edmond; Chernin, Mark; Weintraub, Zalman; Rotschild, Avi; Bitterman, Haim

doi:10.1152/ajpheart.00900.2002

Cited by 54 publications

(48 citation statements)

References 33 publications

(41 reference statements)

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“…Ischemia-reperfusion injury is caused by the increased oxygen supply after a period of ischemia, leading to excess formation of hydroxyl and lipid radicals (Calikoglu et al 2003;Waisman et al 2003). Multiple organ damage after ischemia reperfusion injury has been called as remote organ injury (Nielsen et al 1997;Guler et al 2004;Okutan et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Although the reinstitution of blood flow to ischemic tissues is critical to their salvage, the reperfusion of ischemic organs may paradoxically increase the tissue damage induced by the ischemia (Waisman et al 2003), causing local and systemic destruction. Remote organs with intense micro-capillary systems such as lung and kidney are prone to develop this type of systemic injury (Calikoglu et al 2003;Guler et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Leukocytes exposed to ischemic tissue may reenter the systemic circulation in an activated state upon reperfusion and have been implicated as mediators of remote organ injury. After adhering to endothelium, leukocytes transmigrate across endothelial surfaces into the tissues and exert their toxic effects through the release of proteolytic enzymes and free radicals, resulting in micro-vascular dysfunction and local inflammatory response (Waisman et al 2003). MDA is, in particular, produced by lipid peroxidation and serves as an index of biochemical oxidative damage in the tissue (Gul et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…IR creates large amount of free oxygen radicals released into the blood circulation and results in generalized inflammatory response and systemic damage (Waisman et al 2003). Especially reperfusion period generates toxic oxygen radicals which have deleterious effect on cellular membranes (Cetinus et al 2005).…”

confidence: 99%

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Zinc May Protect Remote Ocular Injury Caused by Intestinal Ischemia Reperfusion in Rats

Özdemir

İnanç

2005

Tohoku J. Exp. Med.

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Remote organ injury represents the oxidative damage, which occurs in various organs away from the tissues exposed to ischemia-reperfusion insult. Hypothesizing that the eye may be susceptible to this type of tissue damage, we investigated the effect of splanchnic ischemia-reperfusion on the chorio-retinal tissue in Sprague-Dawley rats. Four groups, each consisting of 10 male rats, were designed for the study. Ischemia-reperfusion was introduced by clamping superior mesenteric artery for 1 hour, followed by reperfusion for 90 min (IR group). In another group, the same operation was performed except that zinc aspartate (50 mg/kg) was given intra-peritoneally 15 min before the opening of the clamp (Zn-IR group). As control (sham group), the abdomen was opened without any intervention on superior mesenteric artery. The other group was given Zn after opening of abdomen without any intervention on superior mesenteric artery (Zn-sham group). After the choroid and the retina were dissected from the eye, malondialdehyde, superoxide dismutase, catalase and nitric oxide (NO) were measured in the collected eye tissues. We found that the levels of malondialdehyde, an indicator for lipid peroxidation, were higher in the chorio-retinal tissue of IR group compared to Zn-IR, Zn-sham or sham group (p < 0.05). The oxidative stress in Zn-IR group was not higher than that of sham group. Treatment with zinc decreased NO levels but had no noticeable effect on the level of antioxidant enzymes. Our study indicates that remote organ injury is induced in the eye during splanchnic ischemia-reperfusion and that zinc may be beneficial to ameliorate remote ocular injury.remote ocular injury; zinc; IR injury; chorio-retinal oxidation

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Zinc May Protect Remote Ocular Injury Caused by Intestinal Ischemia Reperfusion in Rats

Özdemir

İnanç

2005

Tohoku J. Exp. Med.

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“…Experiments were recorded with a Sony 3CCD color video camera DXC-950P coupled to the microscope and connected to a Sony digital videocassette recorder (DVCAM DSR-30P), and in parallel to a color video monitor (Mennen Medical). Two different types of video recordings were generated, as described in [16] 2) Type II videos: These videos correspond to a series of experiments that studied the behavior of unstained circulating leukocytes in the vascular bed of the mesenteric microcirculation. Their rolling adhesion to the vessel's walls was measured; for an example, see Fig.…”

Section: A Videomicroscopymentioning

confidence: 99%

An automated method for analysis of flow characteristics of circulating particles from in vivo video microscopy

Eden

Waisman²,

Rudzsky³

et al. 2005

IEEE Trans. Med. Imaging

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The behavior of white and red blood cells, platelets and circulating injected particles is one of the main focuses in physiological studies. Most methods used to analyze the circulatory patterns of cells are time consuming. We describe a system named CellTrack, designed for a fully automated tracking of circulating cells and micro-particles and retrieval of their behavioral characteristics.The task of automated blood cell tracking in vessels from in-vivo video is particularly challenging because of the blood cells' non-rigid shapes, the instability inherent in in-vivo videos, the abundance of moving objects and their frequent superposition.To tackle this, the CellTrack system operates on two levels: first, a global processing module extracts vessel borders and center lines based on color and temporal patterns. This enables the computation of the approximate direction of the blood flow in each vessel. Second, a local processing module extracts the locations and velocities of circulating cells. This is performed by artificial neural network classifiers that are designed to detect specific types of blood cells and micro-particles. The motion correspondence problem is then resolved by a novel algorithm that incorporates both the local and the global information. The system has been tested on a series of in-vivo color video recordings of rat mesentery. Our results show that the synergy between the global and local information enables CellTrack to overcome many of the difficulties inherent in tracking methods that rely solely on local information. A comparison was made between manual measurements and the automatically extracted measurements of leukocytes and fluorescent microspheres circulatory velocities. This comparison revealed an accuracy of 97%. CellTrack also enabled a much larger volume of sampling in a fraction of time compared to the manual measurements. All these results suggest that our method can in fact constitute a reliable replacement for manual extraction of blood flow characteristics from in-vivo videos.

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Hyperbaric oxygen therapy attenuates neuropathic hyperalgesia in rats and idiopathic trigeminal neuralgia in patients

Niu

Liu

et al. 2012

European Journal of Pain

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Background: Neuropathic pain after nerve injury is severe and intractable, and current drug and non-drug therapies offer very limited pain relief. Hyperbaric oxygen (HBO2) has been clinically used for protection of the nervous system after acute injury. We investigated whether HBO2 treatment could prevent and/or attenuate neuropathic pain in animals and in patients. Methods: Mechanical allodynia and thermal hyperalgesia and neurochemical alterations of neuropathic pain were analysed in male, adult, Sprague-Dawley rats with sciatic nerve injury. Clinical trials were conducted in patients with idiopathic trigeminal neuralgia. Results: Repetitive HBO2 treatment [a combination of pressure at 3 atmosphere absolute (ATA) and pure oxygen] greatly inhibited behavioural signs of neuropathic pain manifested as thermal hyperalgesia and mechanical allodynia. Such an HBO2 treatment also inhibited nerve injury-induced induction of c-Fos and activation of astrocytes and increased phosphorylation of NR2B receptor and the subsequent Ca 2+-dependent signals in rats. Neither high pressure (up to 3 ATA) nor pure oxygen alone resulted in analgesic effect. In clinical trials, one course of HBO2 therapy (10 consecutive days) produced a rapid-onset, dose-dependent and long-lasting analgesic effects evidenced by the decreased doses of carbamazepine required for keeping patient pain at a minimum and decreased scores of visual analogue scales, which was used for patient's self-evaluation. Conclusions: These findings support that HBO2 therapy is an effective approach for treating neuropathic pain in both animals and human beings and suggest that neural protection, anti-inflammation and inhibition of nerve injury-induced altered neural activity may contribute to the analgesic effect of HBO2 therapy.

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Effects of hyperoxia on local and remote microcirculatory inflammatory response after splanchnic ischemia and reperfusion

Cited by 54 publications

References 33 publications

Zinc May Protect Remote Ocular Injury Caused by Intestinal Ischemia Reperfusion in Rats

Zinc May Protect Remote Ocular Injury Caused by Intestinal Ischemia Reperfusion in Rats

An automated method for analysis of flow characteristics of circulating particles from in vivo video microscopy

Hyperbaric oxygen therapy attenuates neuropathic hyperalgesia in rats and idiopathic trigeminal neuralgia in patients

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