Effects of hypergravity conditions on elongation growth and lignin formation in the inflorescence stem of Arabidopsis thaliana

Tamaoki, Daisuke; Karahara, Ichirou; Schreiber, Lukas; Wakasugi, Tatsuya; Yamada, Kyoji; Kamisaka, Seiichiro

doi:10.1007/s10265-005-0243-1

Cited by 36 publications

(53 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A possible explanation for this effect would be that it is the consequence of modifications in the cell wall, which becomes shorter, thicker and more rigid due to the accumulation of xyloglucans, as a countermeasure against the additional gravitational force (Soga 2004). Furthermore, it has been shown that hypergravity promotes an increase in Arabidopsis thaliana secondary cell wall lignifications (Tamaoki et al 2006). …”

Section: Discussionmentioning

confidence: 99%

A Hypergravity Environment Induced by Centrifugation Alters Plant Cell Proliferation and Growth in an Opposite Way to Microgravity

Manzano

Herranz

Loon

et al. 2012

Microgravity Sci. Technol.

View full text Add to dashboard Cite

Seeds of Arabidopsis thaliana were exposed to hypergravity environments (2g and 6g) and germinated during centrifugation. Seedlings grew for 2 and 4 days before fixation. In all cases, comparisons were performed against an internal (subjected to rotational vibrations and other factors of the machine) and an external control at 1g. On seedlings grown in hypergravity the total length and the root length were measured. The cortical root meristematic cells were analyzed to investigate the alterations in cell proliferation, which were quantified by counting the number of cells per millimeter in the specific cell files, and cell growth, which were appraised through the rate of ribosome biogenesis, assessed by morphological and morphometrical parameters of the nucleolus. The expression of cyclin B1, a key regulator of entry in mitosis, was assessed by the use of a CYCB1:GUS genetic construction. The results showed significant differences in some of these parameters when comparing the 1g internal rotational control with the 1g external control, indicating that the machine by itself was a source of alterations. When the effect of hypergravity was isolated from other environmental factors, by comparing the experimental conditions with the rotational control, cell proliferation appeared depleted, cell growth was increased and there was an enhanced expression of cyclin B1. The functional meaning of these effects is that cell proliferation and cell growth, which are strictly associated functions under normal 1g ground conditions, are uncoupled under hypergravity. This uncoupling was also described by us in previous experiments as an effect of microgravity, but in an opposite way. Furthermore, root meristems appear thicker in hypergravity-treated than in control samples, which can be related to changes in the cell wall induced by altered gravity.

show abstract

Section: Discussionmentioning

confidence: 99%

A Hypergravity Environment Induced by Centrifugation Alters Plant Cell Proliferation and Growth in an Opposite Way to Microgravity

Manzano

Herranz

Loon

et al. 2012

Microgravity Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Among them, centrifuge systems have been widely used to analyze the effect of hypergravity perception and response in plants. Exposures to hypergravity values have been shown to affect growth, development and cell wall properties in various plants (Waldron and Brett 1990;Hoson et al 1996;Soga et al 1999a, b;Tamaoki et al 2006;Nakabayashi et al 2006;Bhaskaran et al 2009). In most of the earlier reports, three to five days old seedlings were exposed to higher g values up to 300 g for longer durations and effects were studied.…”

Section: Introductionmentioning

confidence: 96%

Effects of Short-term Hypergravity Exposure on Germination, Growth and Photosynthesis of Triticum aestivum L.

Vidyasagar

Jagtap²,

Dixit

et al. 2014

Microgravity Sci. Technol.

View full text Add to dashboard Cite

Numerous studies have been carried out to investigate the hypergravity effect on plants, where seedlings (4-5 days old) were continuously exposed and grown under hypergravity condition. Here, we have used a novel 'shortterm hypergravity exposure experimental method' where imbibed caryopses (instead of seedlings) were exposed to higher g values ranging from 500 g to 2500 g for a short interval time of 10 minutes and post short-term hypergravity treated caryopses were grown under 1 g conditions for five days. Changing patterns in caryopsis germination and growth, along with various photosynthetic and biochemical parameters were studied. Results revealed the significant inhibition of caryopsis germination and growth in shortterm hypergravity treated seeds over control. Photosynthesis parameters such as chlorophyll content, rate of photosynthesis (P N ), transpiration rate (Evap) and stomatal conductance (Gs), along with intracellular CO 2 concentration (C int ) were found to be affected significantly in 5 days old seedlings exposed to short-term hypergravity treatment. In order to investigate the cause of observed inhibition, we examined the α-amylase activity and antioxidative enzyme activities. α-amylase activity was found to be inhibited, along with the reduction of sugars necessary for germination and earlier growth in short-term hypergravity treated caryopses. The activities of antioxidant enzymes such as catalase and guaiacol peroxidase were increased in short-term hypergravity treated caryopses, suggesting that caryopses might have experienced oxidative stress upon short-term hypergravity exposure.

show abstract

“…Also, hypergravity inhibits elongation growth of shoots by decreasing the cell wall extensibility as well as by the thickening of cell wall [6]. Stem elongation in Arabidopsis thaliana inflorescence stems was suppressed while dry weight of the inflorescence stems increased in hypergravity [7]. However, most of these studies on hypergravity were carried out on seedlings up to 500 g [4,8,9].…”

Section: Introductionmentioning

confidence: 99%

Effect of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings

Vidyasagar¹

2016

AIP Conference Proceedings

View full text Add to dashboard Cite

An investigation of the effects of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings has been done. The UV-C irradiation periods of exposure were 30, 60, 90, 120 and 180 minutes. The hypergravity used were 1000 g, 2000 g and 2500 g. The combination treatment is UV-C irradiation for 180 min followed by each hypergravity. The results showed that irradiation of UV-C on wheat seeds have stimulated the seed germination, but hypergravity and combination treatments on wheat seeds have inhibited the seed germination. Those treatments gave negative effects to growth rate, the content of chlorophyll a, b and total chlorophyll of wheat seedlings.

show abstract

Effects of hypergravity conditions on elongation growth and lignin formation in the inflorescence stem of Arabidopsis thaliana

Cited by 36 publications

References 27 publications

A Hypergravity Environment Induced by Centrifugation Alters Plant Cell Proliferation and Growth in an Opposite Way to Microgravity

A Hypergravity Environment Induced by Centrifugation Alters Plant Cell Proliferation and Growth in an Opposite Way to Microgravity

Effects of Short-term Hypergravity Exposure on Germination, Growth and Photosynthesis of Triticum aestivum L.

Effect of UV-C radiation and hypergravity on germination, growth and content of chlorophyll of wheat seedlings

Contact Info

Product

Resources

About