Effects of Hyperbaric Oxygen on Proliferative and Apoptotic Activities and Reactive Oxygen Species Generation in Mouse Fibroblast 3T3/J2 Cell Line

Conconi, Maria Teresa; Baiguera, Silvia; Guidolin, Diego; Furlan, Claudia; Menti, Anna Michela; Vigolo, Simonetta; Belloni, Anna S.; Parnigotto, Pier Paolo; Nussdorfer, Gastone G.

doi:10.2310/6650.2003.39201

Cited by 34 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, exposure of cultured fibroblasts to HBO 2 at pressures ranging from 1.5 to 3.0 ATA for 90 to 120 minutes inhibits the cell growth rate, 35 suppresses collagen synthesis, 36 and accelerates cell death. 37 Our results showed that daily HBO 2 treatment at 2.5 ATA for 90 minutes on 3 consecutive days significantly suppressed BrdU incorporation, indicating a reduced proliferation rate in fibroblasts cultured with normal DME medium. HBO 2 exposure also caused more cell death (Figure 4) and an overall significant decrease in total cell survival.…”

Section: Discussionsupporting

confidence: 48%

“…A small amount of ROS production may trigger mechanisms leading to endothelial proliferation and neovascularization. 18 Conconi et al 37 demonstrated that exposure of cultured fibroblasts to a short period of HBO 2 increased proliferation, whereas prolonged HBO 2 exposure inhibited cell growth. These findings suggest that oxygen tension is the critical factor determining the direct effects of oxygen on fibroblasts whose proliferation is suppressed by exposure to a high glucose concentration.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hyperbaric oxygen attenuates cell growth in skin fibroblasts cultured in a high‐glucose medium

Lin

Chu²,

Perng

et al. 2008

Wound Repair Regeneration

View full text Add to dashboard Cite

Hyperglycemia and hypoxia synergistically retard diabetic wound healing. We investigated the direct effect of hyperbaric and normobaric hyperoxia on skin fibroblasts cultured in a high-glucose medium. Detroit 551 human dermal fibroblasts cultured in Dulbecco's modified Eagle's medium containing d-glucose had reduced cell survival compared with cells grown in normal glucose medium; survival was 27.5+/-3.8% lower in 25 mM glucose and 30.6+/-3.7% lower in 50 mM glucose. Cell survival decreased because of inhibition of cell proliferation and enhanced cell death. Daily hyperbaric oxygen therapy at 2.5 atmosphere absolute for 90 minutes on 3 consecutive days reduced cell proliferation and increased cell death in normal cultured fibroblasts. Hyperbaric oxygen therapy and high-glucose medium had a synergistic effect and reduced survival by 37.6+/-4.4% (25 mM glucose) and 39.6+/-5.1% (50 mM glucose). The effects of hyperbaric oxygen and high-glucose medium were associated with overproduction of reactive oxygen species. Our results suggest that direct exposure of skin fibroblasts to hyperbaric oxygen affects cell growth and superimposes the toxic effect of high glucose. This cytotoxicity may be related to the production of reactive oxygen species in the fibroblasts.

show abstract

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Hyperbaric oxygen attenuates cell growth in skin fibroblasts cultured in a high‐glucose medium

Lin

Chu²,

Perng

et al. 2008

Wound Repair Regeneration

View full text Add to dashboard Cite

show abstract

“…This study has shown that survival in vitro of 3 T3 fibroblast cultures was not impaired, and that IEC cells incubated with oxygen-producing sutures, and cultured under hypoxic conditions, had contractility responses comparable to those of cells cultured under normoxia. These results confirm the findings of an earlier study 22 that demonstrated a proliferative effect of hyperbaric oxygen (HBO) on murine 3 T3 fibroblasts, in spite of enhanced ROS production. However, ROS were demonstrated to play a beneficial key role in intestinal wound healing by stimulating cell attraction, migration and adhesion, and immune cell activation, effects that appear to be potentiated by the presence of commensal bacteria 23,24 .…”

Section: Discussionsupporting

confidence: 91%

Effect of oxygen‐producing suture material on hypoxic colonic anastomoses in an experimental model

et al. 2019

View full text Add to dashboard Cite

BackgroundAnastomotic leak remains a significant cause of morbidity and mortality after colorectal surgery. Among multiple risk factors considered, hypoxia–ischaemia is considered to be a primary cause of intestinal anastomotic leakage. The aim of this experimental study was to assess safety, usability for surgical tasks, and efficacy of a newly developed oxygen‐producing suture material in the healing of colonic anastomoses under critical conditions.MethodsAn oxygen‐producing suture material was produced that is capable of releasing oxygen directly into the surrounding tissue. Off‐the‐shelf sutures loaded with calcium peroxide nano‐crystals and covered with poly(d,l‐lactide‐co‐glycolide) were assessed in vitro and in a rat model of hypoxic colonic anastomosis.Results In vitro assessment showed that these sutures can increase oxygen levels in a hypoxic environment. Potential oxygen byproducts did not seem to have a negative impact on the viability of intestinal cells. The use of oxygen‐producing sutures in vivo resulted in increased tissue oxygen saturation, measured by visible light spectroscopy, and increased mechanical stability of the anastomosis.ConclusionOxygen‐producing suture material increased tissue oxygen saturation and mechanical stability of colonic anastomosis in a rat model.Surgical relevanceLeakage of anastomoses remains a significant problem after colorectal surgery.An oxygen‐producing suture material was produced that was shown to be safe in vitro and significantly improved several aspects of healing of colonic anastomoses in an animal model.Oxygen‐producing suture material or stapler devices might help to reduce the risk of anastomotic leak of intestinal anastomoses under physiological and critical conditions such as hypoxia.

show abstract

“…As an overall effect, at least in rodents, hyperbaric oxygenation appears to be antiinflammatory since it increases the susceptibility to respiratory infections [23,24] and delays allograft rejection [25][26][27]. While hyperbaric oxygenation may inhibit apoptosis in models of ischemic brain injury [28,29] and ischemic wound healing [30] possibly due to limiting tissue hypoxia, a single exposure to high partial oxygen pressure was observed to enhance apoptosis mouse fibroblasts [31], Jurkat-T-cells, HL-60 cells, murine thymocytes [32] and NCI-H929 [33].…”

Section: Introductionmentioning

confidence: 98%

Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism

Weber

Koch²,

Kankeleit

et al. 2008

Apoptosis

View full text Add to dashboard Cite

During therapeutic hyperbaric oxygenation lymphocytes are exposed to high partial pressures of oxygen. This study aimed to analyze the mechanism of apoptosis induction by hyperbaric oxygen. For intervals of 0.5-4 h Jurkat-T-cells were exposed to ambient air or oxygen atmospheres at 1-3 absolute atmospheres. Apoptosis was analyzed by phosphatidylserine externalization, caspase-3 activation and DNA-fragmentation using flow cytometry. Apoptosis was already induced after 30 min of hyperbaric oxygenation (HBO, P < 0.05). The death receptor Fas was downregulated. Inhibition of caspase-9 but not caspase-8 blocked apoptosis induction by HBO. Hyperbaric oxygen caused a loss of mitochondrial membrane potential and caspase-9 induction. The mitochondrial pro-survival protein Bcl-2 was upregulated, and antagonizing Bcl-2 function potentiated apoptosis induction by HBO. In conclusion, a single exposure to hyperbaric oxygenation induces lymphocyte apoptosis by a mitochondrial and not a Fas-related mechanism. Regulation of Fas and Bcl-2 may be regarded as protective measures of the cell in response to hyperbaric oxygen.

show abstract

Effects of Hyperbaric Oxygen on Proliferative and Apoptotic Activities and Reactive Oxygen Species Generation in Mouse Fibroblast 3T3/J2 Cell Line

Cited by 34 publications

References 0 publications

Hyperbaric oxygen attenuates cell growth in skin fibroblasts cultured in a high‐glucose medium

Hyperbaric oxygen attenuates cell growth in skin fibroblasts cultured in a high‐glucose medium

Effect of oxygen‐producing suture material on hypoxic colonic anastomoses in an experimental model

Hyperbaric oxygen induces apoptosis via a mitochondrial mechanism

Contact Info

Product

Resources

About