Effects of hydroxyapatite microparticle morphology on bone mesenchymal stem cell behavior

Abstract

Hydroxyapatite (HAp) particles with different morphology but similar physicochemical properties were prepared by a novel and facile route. The interaction between the particles with BMSCs was investigated, and results revealed that particles’ shape has a strong influence on cellular behavior.

Synthesis of Nanostructured Hydroxyapatite via Controlled Hydrothermal Route

“…The achieving of a precise controlling of morphology, geometry and size, over the crystallographic and chemical structure of HA in hydrothermal conditions is related with a largest range of useful work conditions, in particular the temperature. The literature reports many variety of hydrothermal methods for the realization of HA-nanostructured materials, in a wide range of dimension and variety of morphological features of the crystals, for example, rods [20,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46], needles [29,30,32,[47][48][49][50][51][52][53], wires [18,[54][55][56][57], whiskers/fibers [23,47,49,[58][59][60], sheets [55,61,62], plates [23, 44, 47-49, 54, 63, 64], and organized rod spheres [18,…”

“…It is the thermodynamic driving force that governs the formation mechanism of HA crystals, through nucleation and growth processes, and dictates the final crystal size distribution [44]. Nucleation and crystal growth are often competing mechanisms determining for the final crystal size and size distribution.…”

“…Electrolytes Acetamide (AA) [60], urea [29,42,58,68], Na-citrate [37,38,44], propionamide (PA) [59], etc.…”

Nano-hydroxyapatite reinforced polyphenylene sulfide biocomposite with superior cytocompatibility and in vivo osteogenesis as a novel orthopedic implant

“…Combining polymers with bioactive inorganic materials, such as b-tricalcium phosphate (b-TCP), 21,22 hydroxyapatite (HA) 23,24 and titanium dioxide (TiO 2 ) 25,26 through blending is considered a fascinating and advisable strategy to built biocomposite with appropriate properties for orthopedic purposes, because native bone per se is an organic-inorganic biocomposite organized on micro-and nanoscale. 27 In view of the immanent osteoconduction and osseointegration potence, 28 nano-hydroxyapatite (nano-HA, Ca 10 (PO 4 ) 6 -(OH) 2 ), the chief mineral component of bone matrix, is one of the most appealing inorganic materials for applications in bone regenerative medicine, and therefore has been widely employed in hard tissue engineering to promote biological properties of bioinert polymer via compound approach. 29,30 For instance, Yubao Li and his group previously constructed polyamide 66/ nano-HA (PA66/nano-HA) composites by incorporating nano-HA particles within PA66 matrix to improve its stiffness, biocompatibility, and osteogenic differentiation activity.…”

In vivo comparison of biomineralized scaffold-directed osteogenic differentiation of human embryonic and mesenchymal stem cells

“…The scaffold-mediated prevalent osteogenic differentiation of donor cells could improve their in vivo survival. While biomaterials containing CaP minerals have been shown to promote osteogenic commitment of progenitor cells and in vivo bone tissue repair, recent studies suggest that their osteoinductivity could be determined by various physical and chemical parameters, such as the topographical cues, chemical components of the mineral phase, their crystalline nature, and dissolution kinetics of the minerals [27,[39][40][41][42]. The dissociation products of CaP minerals, Ca 2+ and PO 4 3− ions, have been shown to promote osteogenic commitment of stem cells through various cell surface ion channels, such as L-type voltage-dependent calcium channels (VDCC L ) and solute carrier family 20 (phosphate transporter), member 1 (SLC20a1) [43][44][45].…”