Effects of hydroxy compounds on gel formation of gelatin

Fujitsu, Masako; Hattori, Manabu; Tamura, Takamitsu

doi:10.1007/s003960050053

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…The results showed a red shift in amide I band to the lower wavenumber indicating the hydrogen bonding of the CO in gelatin to the silanol hydrogen [38]. These interactions could be used to induce physical cross-linkage [39, 40]. The formation of the continuous aminosilica phases within GEMOSIL nanocomposites relies on the cross-linkage of the enTMOS.…”

Section: Discussionmentioning

confidence: 99%

Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration

Chiu

Ferreira

Luo

et al. 2012

J Mater Sci: Mater Med

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Hydroxyapatite-gelatin modified siloxane (GEMOSIL) nanocomposite was developed by coating, kneading and hardening processes to provide formable scaffolding for alloplastic graft applications. The present study aims to characterize scaffolding formability and mechanical properties of GEMOSIL, and to test the in vitro and in vivo biocompatibility of GEMOSIL. Buffer Solution initiated formable paste followed by the sol-gel reaction led to a final hardened composite. Results showed the adequate coating of aminosilane, 11–19 wt%, affected the cohesiveness of the powders and the final compressive strength (69 MPa) of the composite. TGA and TEM results showed the effective aminosilane coating that preserves hydroxyapatite-gelatin nanocrystals from damage. Both GEMOSIL with and without titania increased the mineralization of preosteoblasts in vitro. Only did titania additives revealed good in vivo bone formation in rat calvarium defects. The scaffolding formability, due to cohesive bonding among GEMOSIL particles, could be further refined to fulfill the complicated scaffold processes.

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Section: Discussionmentioning

confidence: 99%

Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration

Chiu

Ferreira

Luo

et al. 2012

J Mater Sci: Mater Med

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“…39,40 Using the equation reported by Fujitsu et al, the relative helical content ([C] rel ) of gelatin in the presence of polyenes was estimated. 41 Positive or negative (C) rel indicates increased or decreased triple helical content, respectively. (C) rel at 10°C for amphotericin B-and natamycin-loaded gelatin was 0.8 and 2.4, respectively, indicating greater stability of triple helix structure in the presence of natamycin.…”

Section: Interaction Of Gelatin With Antifungalsmentioning

confidence: 99%

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Lakshminarayanan

Sridhar

Loh

et al. 2014

IJN

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Topical application of antifungals does not have predictable or well-controlled release characteristics and requires reapplication to achieve therapeutic local concentration in a reasonable time period. In this article, the efficacy of five different US Food and Drug Administration-approved antifungal-loaded (amphotericin B, natamycin, terbinafine, fluconazole, and itraconazole) electrospun gelatin fiber mats were compared. Morphological studies show that incorporation of polyenes resulted in a two-fold increase in fiber diameter and the mats inhibit the growth of yeasts and filamentous fungal pathogens. Terbinafine-loaded mats were effective against three filamentous fungal species. Among the two azole antifungals compared, the itraconazole-loaded mat was potent against Aspergillus strains. However, activity loss was observed for fluconazole-loaded mats against all of the test organisms. The polyene-loaded mats displayed rapid candidacidal activities as well. Biophysical and rheological measurements indicate strong interactions between polyene antifungals and gelatin matrix. As a result, the polyenes stabilized the triple helical conformation of gelatin and the presence of gelatin decreased the hemolytic activity of polyenes. The polyene-loaded fiber mats were noncytotoxic to primary human corneal and sclera fibroblasts. The reduction of toxicity with complete retention of activity of the polyene antifungal-loaded gelatin fiber mats can provide new opportunities in the management of superficial skin infections.

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“…The polymeric radicals generated by the plasma components can interact with the OH species, leading to the formation of hydroxyl compounds onto the polymeric fibers of the mats, which, in turn, lead to the increase of the number of crosslinking junctions through hydrogen bonding, as previously found by Fijitsu et al . in gelatin gels34.…”

Section: Resultsmentioning

confidence: 99%

Atmospheric Pressure Non-Equilibrium Plasma as a Green Tool to Crosslink Gelatin Nanofibers

Liguori

Bigi

Colombo

et al. 2016

Sci Rep

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Electrospun gelatin nanofibers attract great interest as a natural biomaterial for cartilage and tendon repair despite their high solubility in aqueous solution, which makes them also difficult to crosslink by means of chemical agents. In this work, we explore the efficiency of non-equilibrium atmospheric pressure plasma in stabilizing gelatin nanofibers. We demonstrate that plasma represents an innovative, easy and environmentally friendly approach to successfully crosslink gelatin electrospun mats directly in the solid state. Plasma treated gelatin mats display increased structural stability and excellent retention of fibrous morphology after immersion in aqueous solution. This method can be successfully applied to induce crosslinking both in pure gelatin and genipin-containing gelatin electrospun nanofibers, the latter requiring an even shorter plasma exposure time. A complete characterization of the crosslinked nanofibres, including mechanical properties, morphological observations, stability in physiological solution and structural modifications, has been carried out in order to get insights on the occurring reactions triggered by plasma.

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Effects of hydroxy compounds on gel formation of gelatin

Cited by 14 publications

References 22 publications

Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration

Direct scaffolding of biomimetic hydroxyapatite-gelatin nanocomposites using aminosilane cross-linker for bone regeneration

Interaction of gelatin with polyenes modulates antifungal activity and biocompatibility of electrospun fiber mats

Atmospheric Pressure Non-Equilibrium Plasma as a Green Tool to Crosslink Gelatin Nanofibers

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