Effects of hydrogen-rich water prepared by alternating-current-electrolysis on antioxidant activity, DNA oxidative injuries, and diabetes-related markers

Abstract

Hydrogen-rich water is conventionally prepared by direct current-electrolysis, but has been not or scarcely prepared by alternating current (AC)-electrolysis. The AC preparations from tap water for 20–30 minutes exhibit a dissolved hydrogen concentration of 1.55 mg/L, which was close to the theoretical maximum value of 1.6 mg/L. These preparations also displayed an oxidation-reduction potential of –270 mV (tap water: +576 mV) and pH of 7.7–7.8, being closer to physiological values of body fluids than general types of direct current-electrolytic hydrogen-rich water. We examined whether AC-electrolytic hydrogen-water is retained for hydrogen-abundance after boiling or for antioxidant abilities, and whether the oral administration of this water is clinically effective for diabetes and prevention against systemic DNA-oxidative injuries. 5,5-Dimethyl-1-pyrroline-N-oxide spin trapping and electron spin resonance revealed that the hydrogen-rich water generated by AC-electrolysis exhibited hydroxyl-radical-scavenging activities. Laser nanoparticle tracking method revealed that nanoparticle suspensions as abundant as 5.4 × 10 7 /mL were efficiently retained (up to 3.5 × 10 7 /mL) even after boiling for 10 minutes, being thermodynamically contrary to Henry's law. Oral intake of hydrogen-rich water, 1500 mL per day, lasted for 8 weeks in nine people with the diabetes-related serum markers beyond the normal ranges. The subjects exhibited significant tendencies for the decreased fasting blood glucose and fructosamine, and for the increased 1,5-anhydro-D-glucitol, concomitantly with significant decreases in urinary 8-hydroxy-2-deoxyguanosine contents and its rate of generation. Hydrogen-rich water prepared by AC-electrolysis may be effective in improving diverse diabetes-related markers and systemic DNA oxidative injuries through the formation of abundant heat-resistant nanobubbles and the increased hydrogen concentrations. The study protocol was officially approved by the Medical Ethics Committee of the Japanese Center for Anti-Aging Medical Sciences (approval No. 01S02) on September 15, 2009.

Molecular hydrogen is a promising therapeutic agent for pulmonary disease

“…The oral intake of HRS, which is hydrogen dissolved in water, is a portable, safe, and convenient method for administration (Sun et al, 2012;Koyama et al, 2014). Under normal atmospheric pressure and at room temperature, hydrogen can be dissolved in water up to 0.8 mmol/L (1.6 mg/L) (Asada et al, 2020). The injection of HRS can provide highly accurate hydrogen doses.…”

Role of Molecular Hydrogen in Ageing and Ageing-Related Diseases

“…Similarly, in cigarette smoke- (CS-) induced emphysema, H 2 significantly decreased phosphorylated histone H2AX and 8-OHdG levels, which are markers of oxidative DNA damage [ 51 ]. Oral administration of water containing hydrogen-rich saline (HRS) prepared in alternating current electrolysis was effective for preventing systemic oxidative DNA injuries and for clinical diabetes treatment [ 52 ]. These findings suggest that H 2 can potentially intervene in accumulation of genetic damage in the living body caused by oxidative stress and alleviate the ageing process.…”

Effect of hydrogen-rich water on periodontal disease, interleukin and blood glucose levels in diabetic

“…15,16,17 Molecular hydrogen has been identified as one of the antioxidants that can reduce oxidative stress. [18][19][20][21][22] Drinking water that contains a therapeutic hydrogen dose of hydrogen-rich water (HRW) is an easy method of sending molecular hydrogen into the body. Several studies on HRW show that it can reduce blood sugar levels in DM patients.…”