Effects of hydrogen in headspace and bicarbonate in media on rumen fermentation, methane production and methanogenic population using in vitro gas production techniques

Qiao, Jing; Tan, Zhiliang; Guan, Le Luo; Tang, Shaoxun; Zhou, Chuanshe; Han, Xuefeng; Wang, M.; Kang, Jinhe; He, Zhixiong

doi:10.1016/j.anifeedsci.2015.05.004

Cited by 15 publications

(9 citation statements)

References 27 publications

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“…Indeed, no overall correlation between variables was observed in the present study in agreement with Wang et al ( 2016 ), whereas positive correlations were observed within treatments (CON: r = 0.80, P < 0.001; NIT: r = 0.54, P = 0.014; NOP: r = 0.74, P < 0.001; AQ: r = 0.67, P = 0.001; Supplementary Figure 1 ), highlighting the specificity of each methanogenesis inhibitor on rumen fermentation. Qiao et al ( 2015 ) also observed a positive response in CH 4 production to the replacement of headspace N 2 with H 2 in in vitro mixed batch cultures.…”

Section: Discussionmentioning

confidence: 91%

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

2017

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A decrease in methanogenesis is expected to improve ruminant performance by allocating rumen metabolic hydrogen ([2H]) to more energy-rendering fermentation pathways for the animal. However, decreases in methane (CH4) emissions of up to 30% are not always linked with greater performance. Therefore, the aim of this study was to understand the fate of [2H] when CH4 production in the rumen is inhibited by known methanogenesis inhibitors (nitrate, NIT; 3-nitrooxypropanol, NOP; anthraquinone, AQ) in comparison with a control treatment (CON) with the Rumen Simulation Technique (RUSITEC). Measurements started after 1 week adaptation. Substrate disappearance was not modified by methanogenesis inhibitors. Nitrate mostly seemed to decrease [2H] availability by acting as an electron acceptor competing with methanogenesis. As a consequence, NIT decreased CH4 production (−75%), dissolved dihydrogen (H2) concentration (−30%) and the percentages of reduced volatile fatty acids (butyrate, isobutyrate, valerate, isovalerate, caproate and heptanoate) except propionate, but increased acetate molar percentage, ethanol concentration and the efficiency of microbial nitrogen synthesis (+14%) without affecting gaseous H2. Nitrooxypropanol decreased methanogenesis (−75%) while increasing both gaseous and dissolved H2 concentrations (+81% and +24%, respectively). Moreover, NOP decreased acetate and isovalerate molar percentages and increased butyrate, valerate, caproate and heptanoate molar percentages as well as n-propanol and ammonium concentrations. Methanogenesis inhibition with AQ (−26%) was associated with higher gaseous H2 production (+70%) but lower dissolved H2 concentration (−76%), evidencing a lack of relationship between the two H2 forms. Anthraquinone increased ammonium concentration, caproate and heptanoate molar percentages but decreased acetate and isobutyrate molar percentages, total microbial nitrogen production and efficiency of microbial protein synthesis (−16%). Overall, NOP and AQ increased the amount of reduced volatile fatty acids, but part of [2H] spared from methanogenesis was lost as gaseous H2. Finally, [2H] recovery was similar among CON, NOP and AQ but was largely lower than 100%. Consequently, further studies are required to discover other so far unidentified [2H] sinks for a better understanding of the metabolic pathways involved in [2H] production and utilization.

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Section: Discussionmentioning

confidence: 91%

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

2017

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“…Similarly, Patra & Yu (8) found that including gH 2 in the initial culture headspace unexpectedly resulted in lower propionate molar percentage. Similarly, Qiao et al (10) found that increasing gH 2 in the headspace of batch cultures actually increased acetate molar percentage and the acetate:propionate ratio, and decreased propionate molar percentage. Infusion of gH 2 in the rumen of dairy cows did not result in changes in total VFA concentration or VFA profile (11) .…”

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confidence: 82%

“…However, when the effect of direct addition of H 2 as gas (gaseous H 2 , gH 2 ) has been studied in in vitro batch cultures (8)(9)(10) and in vivo (11) , the results observed in acetate and Abbreviations: CH 4 , methane; CO 2 , carbon dioxide; dCH 4 , dissolved CH 4 ; dCO 2 , dissolved carbon dioxide; dH 2 , dissolved H 2 ; gCO 2 , gaseous CO2; gH 2 , gaseous H 2 ; H 2 , molecular hydrogen; OUT, operational taxonomic units; VFA, volatile fatty acid.…”

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confidence: 99%

Molecular hydrogen generated by elemental magnesium supplementation alters rumen fermentation and microbiota in goats

Wang

Zhang

et al. 2017

Br J Nutr

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We tested the hypotheses that supplementation of a diet with elemental Mg increases ruminal dissolved H2 (dH2) in rumen fluid, which in turn alters rumen fermentation and microbial community in goats. In a randomised block design, twenty growing goats were allocated to two treatments fed the same basal diet with 1·45 % Mg(OH)2 or 0·6 % elemental Mg. After 28 d of adaptation, we collected total faeces to measure total tract digestibility, rumen contents to analyse fermentation end products and microbial groups, and measured methane (CH4) emission using respiration chambers. Ruminal Mg2+ concentration was similar in both treatments. Elemental Mg supplementation increased dH2 at 2·5 h post morning feeding (+180 %, P<0·001). Elemental Mg supplementation decreased total volatile fatty acid concentration (-8·6 %, P<0·001), the acetate:propionate ratio (-11·8 %, P<0·03) and fungal copy numbers (-63·6 %, P=0·006), and increased propionate molar percentage (+11·6 %, P<0·001), methanogen copy numbers (+47·9 %, P<0·001), dissolved CH4 (+35·6 %, P<0·001) and CH4 emissions (+11·7 %, P=0·03), compared with Mg(OH)2 supplementation. The bacterial community composition in both treatments was overall similar. Ruminal dH2 was negatively correlated with acetate molar percentage and fungal copy numbers (P<0·05), and positively correlated with propionate molar percentage and methanogen copy numbers (P<0·05). In summary, elemental Mg supplementation increased ruminal dH2 concentration, which inhibited rumen fermentation, enhanced methanogenesis and seemed to shift fermentation pathways from acetate to propionate, and altered microbiota by decreasing fungi and increasing methanogens.

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“…It may affect rumen nitrogen metabolism as rumen microbes can utilize atmospheric N 2 [5][6][7] and thus may confound the obtained results. But the previous studies which investigated the effects of headspace N 2 with carbon dioxide (CO 2 ) or dihydrogen on rumen fermentation characteristics in batch culture system found no changes in ammonia nitrogen (NH 3 -N) between the headspace gas compositions [8,9]. These results could be resulted from the gas used for the comparison.…”

Section: Introductionmentioning

confidence: 95%

Effects of nitrogen gas flushing in comparison with argon on rumen fermentation characteristics in in vitro studies

Park¹,

Lee²

2020

J Anim Sci Technol

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In rumen in vitro experiments, although nitrogen gas (N 2 ) flushing has been widely used, its effects on rumen fermentation characteristics are not clearly determined. The present study is the first to evaluate the effects of N 2 flushing on rumen fermentation characteristics in in vitro batch culture system by comparing with new applicable non-metabolizable gas: argon (Ar). The rumen fluid was taken from two Korean native heifers followed by incubation for 3, 9, 12, and 24 h with N 2 or Ar flushing. As a result, in all incubation time, N 2 flushing resulted in higher total gas production than Ar flushing (p < 0.01). Additionally, in N 2 flushing group, ammonia nitrogen was increased (p < 0.01). However, volatile fatty acids profiles and pH were not affected by the flushing gases (p > 0.05). In conclusion, the present study demonstrated that N 2 flushing can influence the rumen nitrogen metabolism via increased ammonia nitrogen concentration and Ar flushing can be used as a new alternative flushing gas.

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Effects of hydrogen in headspace and bicarbonate in media on rumen fermentation, methane production and methanogenic population using in vitro gas production techniques

Cited by 15 publications

References 27 publications

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

Molecular hydrogen generated by elemental magnesium supplementation alters rumen fermentation and microbiota in goats

Effects of nitrogen gas flushing in comparison with argon on rumen fermentation characteristics in in vitro studies

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