Effects of hyaluronic acid on the development of 1- and 2-cell porcine embryos to the blastocyst stage in vitro

Miyano, Takashi; Hirooka, Rina; Kano, Koichiro; Miyake, Masakazu; Kusunoki, Hiroshi; Kato, Seishiro

doi:10.1016/0093-691x(94)90488-5

Cited by 54 publications

(38 citation statements)

References 30 publications

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“…These authors reported that the incorporation of HA in a chemically defined medium clearly demonstrated the effect of HA in the improvement of blastocyst formation. This is in agreement with Miyano et al (1994), who verified that the proportion of degenerated porcine embryos was lower in the presence than in the absence of HA. It has been suggested that HA might benefit embryo development per se or by regulating the action of factors synthesized by the embryo, acting in an autocrine way (Furnus et al, 2003).…”

Section: Discussionsupporting

confidence: 81%

“…It has also been demonstrated that HA delays death and prevents fragmentation of porcine oocytes (Sato et al, 1994;Tunjung et al, 2009) and plays a role in cell migration during the early embryonic development (Peterson et al, 1993). Moreover, HA added to the culture medium supports the development of 1-and 2-cell porcine embryos (Miyano et al, 1994) as well as improves in vitro bovine embryo development to the blastocyst stage (Furnus et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Expression of CD44 in sheep oocytes and preimplantation embryos

Luz¹,

Alcântara-Neto²,

Batista³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult hair ewes submitted to ovarian stimulation treatment. The CD44 mRNA was detected by hemi-nested PCR, after reverse transcription, while proteins were located by indirect immunofluorescence, using anti-human CD44 monoclonal antibody. Human lymphocytes and immature bovine oocytes were used as positive and negative controls, respectively. Assessment of the oocyte nuclear stages as well as classification of the embryonic development stage were made with Hoechst 33342 staining. Indirect immunofluorescence detected CD44 expression on the surface of mature oocytes and embryos; immature oocytes did not take up the stain. These findings were supported by the RT-PCR data, which showed no mRNA templates for CD44, even after two consecutive amplifications, in material from immature oocytes and cumulus cells. The CD44 amplicons were detected after a second hemi-nested PCR in mature oocytes and embryos. The finding of CD44 in mature oocytes and preimplantational embryos could reflect the expression profile of hyaluronic acid during terminal folliculogenesis and preimplantational embryo development in sheep.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Expression of CD44 in sheep oocytes and preimplantation embryos

Luz¹,

Alcântara-Neto²,

Batista³

et al. 2012

Genet. Mol. Res.

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“…Mature porcine oocytes are known to be easily activated by a single electro-stimulation; their frequency of activation has been reported as more (82) 4 (14) 1 ( 4) 23 (82) 4 (14) Total 233 --32 (84)** 6(16)** --43 (19) 168 (72) 22 ( 9) *: Abnormal or not determined oocytes included 12, 7, 5 and 3 eggs at the late anaphase to telophase at 36, 38, 40 and 42 h after electro-stimulation, respectively. The number of chromosomes could not be counted at these stages.…”

Section: Discussionmentioning

confidence: 99%

“…The culture medium was Whitten's medium (WM) [18] supplemented with 4.0 mg/ml bovine serum albumin (BSA; Intergen, Purchase, NY) and 0.5 mg/ml hyaluronic acid (from rooster comb; Seikagaku Co., Tokyo, Japan) (mWM) [6,19]. The osmolarity of the culture medium was kept at 309 mOsmol (mWM309) for the first 48 or 72 h by addition of 26.2 mM NaCl to mWM and then 256 mOsmol (mWM256) until 192 h after El-St [20].…”

Section: Culture Media For Developmentmentioning

confidence: 99%

The Development of Porcine Parthenogenetic Diploid Oocytes with Homogeneous Genomic Components In Vitro.

Thuan

Harayama

Miyake

2002

Journal of Reproduction and Development

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Abstract. The aim of this study was to determine the influence of the homogeneity of genomic components and ploidy on the in vitro development of porcine parthenogenetic oocytes to the blastocyst stage. In vitro matured oocytes were subjected to a single pulse of electro-stimulation (El-St; 100 µsec, 1,500 V/cm) for activation. First, the activated oocytes were cultured for 6, 16, 18, 20 and 22 h after El-St, and examined for the timing of the first cleavage of parthenogenetic haploids. Next, the effects of the timing and duration of cytochalasin B (CB) treatment on inhibition of the first cleavage of haploids were examined in order to produce homogenous parthenogenetic diploids. Then the developmental ability to the blastocyst stage was compared among activated oocytes with genomic components of haploid (without CB treatment), homogeneous diploid (nn-diploid, CB treatment for 6 h from 20 h after El-St), and heterogeneous diploid (nn'-diploid, CB treatment for 4 h immediately after El-St). Most haploids were at the prophase to telophase of the first cleavage between 16 and 22 h after El-St. When the haploids were treated with 5.0 µg/ml CB for 6 h from 20 h after El-St, their first cleavage was efficiently suppressed, and most of them (84%) became diploids. The frequency of parthenogenetic development to the blastocyst stage was significantly lower in haploids (5%) than in nn-diploids (48%, P<0.01) or nn'-diploids (57%, P<0.01). These results shows that ploidy of activated oocytes, but not the homology of genomic components, affects the development of porcine parthenogenetic oocytes to the blastocyst stage. Key words: Porcine parthenogenesis, Ploidy, In vitro development (J. Reprod. Dev. 48: [157][158][159][160][161][162][163][164][165][166] 2002) he genotype of the parthenogenetic embryo var ies d e pe nd ing on the ex per ime ntal conditions and on the postovulatory age of the oocyte. Matured oocytes have two genomic sets of chromosomes, one which is delivered to the egg and the other which is shared by the second polar body. Each chromosome consists of a mixture of paternal and maternal genomic components by the process of crossing-over during the meiotic prophase I. Thus the two genomic sets are not exactly identical. Therefore, the possible genotypes arising from parthenogenetic activation are uniform haploids with the extrusion of the second polar body (n), mosaic haploids through an immediately equal cell-division but not extrusion of the second polar body (n+n'), and heterogeneous diploids without the extrusion of the second polar body (nn') according to the theoretical classification by Kaufman [1]. It has been reported, however, that porcine oocytes matured less than 48 h followed by artificial activation actually yielded activated eggs of uniform haploid type (about 90%) or of heterogeneous diploid type with two haploid (n + n') nuclei or a single diploid (nn') nucleus (a few percentage each), but no mosaic-haploid activated oocytes [2,3].

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“…Although dynamic culture systems may be more similar to Zrt uzuo conditions than static systems, investigators have attempted to create one medium that will support development from one-cell through hatched blastocyst stages. In addition to in viuo and organ (mouse oviduct ;Krisher et al, 1989) culture, improved development of one-cell porcine embryos into blastocyst has been reported with the addition of oviductal fluid (Archibong et d., 1989), oviduct epithelial cells (White et al, 1989), hyaluronic acid (Miyano et al, 1994), glutamine, sorbitol, taurine or hypotaurine (Reed et al, 1991; Petters and Wells, 1993) to the culture medium. Beckmann and Day (1993) reported a medium that supported development from the one-cell to blastocyst stages at high rates and produced live pigs following transfer to recipient animals.…”

Section: Introductionmentioning

confidence: 99%