Effects of hyaluronan on cell proliferation and collagen synthesis: A study of rabbit flexor tendons In vitro

Wiig, Monica; Abrahamsson, Sven-Olof; Lundborg, Göran

doi:10.1016/s0363-5023(96)80010-4

Cited by 66 publications

(44 citation statements)

References 21 publications

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“…In an in vitro cell culture study on cells from rabbit deep flexor tendon, HA inhibited cell proliferation and had no effect on matrix component synthesis. 50 Another in vivo study from the same research group assessed the effect of HA on rabbit deep flexor tendon repair up to 6 weeks postoperation. After investigation of synthesis of DNA, proteoglycan, collagen, and noncollagen protein, they found HA treatment did not alter the rate of cell proliferation or matrix synthesis in healing tendons or sheaths.…”

Section: Discussionmentioning

confidence: 99%

Healing ligament mechanical properties are improved by repair with interpositional allografts but not by concomitant treatment with hyaluronic acid

Thornton¹,

Shao²,

Kuchison³

et al. 2009

Journal Orthopaedic Research

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Healing ligaments have inferior mechanical properties compared to normal ligaments during early healing intervals. The purpose of this study was to investigate if in vivo ligament repair with an interpositional allograft and treatment with hyaluronic acid (HA) would improve the mechanical properties of a medial collateral ligament (MCL) healing from a gap injury. Twenty rabbits were assigned equally to either a donor or recipient group. A gap injury of the MCL was created in both hindlimbs of 10 recipient animals. The right hindlimb was treated with allograft plus HA while the left hindlimb was treated with allograft only. Low-load and high-load mechanical properties, including laxity, relaxation and failure, and histology were evaluated after 6 weeks of healing. Mechanical results were compared to previously published normal MCL and MCL gap scar data. MCL allografts had greater initial force during cyclic relaxation testing and maximum force during failure testing than MCL scars, but were weaker than normal MCLs. Failure stress was the only parameter to demonstrate a statistically significant effect of treatment with HA on the allografts. However, the failure stress of the HA-treated MCL allografts was not different than MCL scars and was less than normal MCLs. In conclusion, interpositional allografts could enhance some mechanical properties of ligament healing but HA, in the way we applied it, did not produce an obvious improvement. ß

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Section: Discussionmentioning

confidence: 99%

Healing ligament mechanical properties are improved by repair with interpositional allografts but not by concomitant treatment with hyaluronic acid

Thornton¹,

Shao²,

Kuchison³

et al. 2009

Journal Orthopaedic Research

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“…It is ubiquitously expressed in the extracellular space, represents a major constituent of the ECM, and has a central role in stability of ECM (Knudson and Knudson, 1993). Numerous cellular processes including cell migration (Chen et al, 1989), adhesion (Klein et al, 1996), and proliferation (Mast et al, 1993;Wiig et al, 1996) are influenced by HA. Thus, HA has an important role in such processes as morphogenesis (Toole, 1997), wound healing (Nishida et al, 1991), inflammation (Weigel et al, 1988), and immune cell trafficking (Mohamadzadeh et al, 1998), as well as in many aspects of tumor biology (Delpech et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor

Bono

Rubin

Higgins

et al. 2001

MBoC

131

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The actin cytoskeleton plays a significant role in changes of cell shape and motility, and interactions between the actin filaments and the cell membrane are crucial for a variety of cellular processes. Several adaptor proteins, including talin, maintain the cytoskeleton-membrane linkage by binding to integral membrane proteins and to the cytoskeleton. Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is a membrane-binding site for talin in peripheral ruffles of spreading cells. To facilitate studies of layilin's function, we have generated a layilin-Fc fusion protein comprising the extracellular part of layilin joined to human immunoglobulin G heavy chain and used this chimera to identify layilin ligands. Here, we demonstrate that layilin-Fc fusion protein binds to hyaluronan immobilized to Sepharose. Microtiter platebinding assays, coprecipitation experiments, and staining of sections predigested with different glycosaminoglycan-degrading enzymes and cell adhesion assays all revealed that layilin binds specifically to hyaluronan but not to other tested glycosaminoglycans. Layilin's ability to bind hyaluronan, a ubiquitous extracellular matrix component, reveals an interesting parallel between layilin and CD44, because both can bind to cytoskeleton-membrane linker proteins through their cytoplasmic domains and to hyaluronan through their extracellular domains. This parallelism suggests a role for layilin in cell adhesion and motility. INTRODUCTIONCell shape and movement form the basis for several biological phenomena such as morphogenesis, invasion, and metastasis. The actin cytoskeleton is a major determinant of changes in cell shape, and interactions between actin filaments and the cell membrane are essential for cell adhesion, spreading, and migration, as well as for signal transduction (Hall, 1998;Schoenwaelder and Burridge, 1999). Several molecules have been recognized as linkers between the actin cytoskeleton and the cell membrane. For example, members of the band 4.1/ERM superfamily (band 4.1, talin, ezrin, radixin, moesin, and merlin, the product of the neurofibromatosis type 2 tumor suppressor gene) are characterized by the presence of a conserved N-terminal membrane-binding domain, which is able to bind to the cytoplasmic region of several transmembrane receptors, including CD44, CD43, ICAM-2, and ICAM-3 (Sainio et al., 1997;Heiska et al., 1998;Yonemura et al., 1998). The C-terminal regions of ERM proteins are thought to bind to actin filaments, thereby linking these transmembrane receptors to the cytoskeleton (reviewed by Mangeat et al., 1999).Layilin is a recently cloned ϳ55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ϳ50-kDa head domain (amino acids 280 -435)...

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“…However these two properties are interdependent. Changes in HA concentration within the extracellular matrix modulates a variety of cellular functions, such as cell migration 12,13 , adhesion 14,15 and proliferation [16][17][18] . Several important medical applications of HA have been discovered for joints degener-ation 7 .…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid increases tendon derived cell viability and proliferation in vitro: comparative study of two different hyaluronic acid preparations by molecular weight

Gallorini

Berardi

Berardocco

et al. 2017

MLTJ

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SummaryBackground: Hyaluronic Acid (HA) has been already approved by Food and Drug Administration (FDA) for osteoarthritis (OA), while its use in the treatment of tendinopathy is still debated. The aim of this study was to evaluate the effects of two different HA on human rotator cuff tendon derived cells in terms of cell viability, proliferation and apoptosis. Methods: An in vitro model was developed on human tendon derived cells from rotator cuff tears to study the effects of two different HA preparations: Sinovial HL® (High-Low molecular weight) (MW: 80-100 kDa) and KDa Sinovial Forte SF (MW: 800-1200), at various concentrations. Tendon derived cells morphology was evaluated after 0, 7 and 14 d of culture. Viability and proliferation were analyzed after 0, 24, and 48 h of culture and apoptosis occurrence was assessed after 24 h of culture.Results: All the HAPs tested here increased viability and proliferation, in a dose-dependent manner and they reduced apoptosis at early stages (24 h) compared to control cells (without HAPs). Conclusions: HAPs enhanced viability and proliferation and counteracted apoptosis in tendon derived cells.

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Effects of hyaluronan on cell proliferation and collagen synthesis: A study of rabbit flexor tendons In vitro

Cited by 66 publications

References 21 publications

Healing ligament mechanical properties are improved by repair with interpositional allografts but not by concomitant treatment with hyaluronic acid

Healing ligament mechanical properties are improved by repair with interpositional allografts but not by concomitant treatment with hyaluronic acid

Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor

Hyaluronic acid increases tendon derived cell viability and proliferation in vitro: comparative study of two different hyaluronic acid preparations by molecular weight

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