Effects of Huoxue Bushen Mixture on skin blood vessel neogenesis and vascular endothelial growth factor expression in hair follicle of C57BL/6 mice

Abstract

Effects of Huoxue Bushen Mixture on skin blood vessel neogenesis and vascular endothelial growth factor expression in hair follicle of C57BL／6 mice Objective ： To investigate the possible stimulat ing mechanism of Huoxue Bushen Mixtu re (HXBSM) ， a tradi tional Chinese compound medicine ， on hair growth of mice via measuring the variance of skin blood vessel neogenesis and vascular endothelial growth factor (VEGF) expression in the hair follicle ． Methods ： Hot rosin and paraffin mixture depilation were used to induce C５７BL／６ mice hair follicle to enter from telogen into anagen ． Ninety C５７BL／６ mice were divided into ３ groups randomly ： HXBSM group ， Yang xue Shengfa Capsule (YXSFC ， another traditional Chinese compound medicine) group and untreated group ． The mice were fed wi th corresponding drugs after modeling ． The hair growth of the mice was observed every day ． Every ten mice out of each group were executed respectively at day ４ ， １１ and day １７ ． Skin blood vessel neogenesis was counted through pathological section and VEGF expression in the hair follicle was measured via immunohistochemical method ． Results ： The number of local blood vessel neogenisis in the HXBSM group observed was larger than that in the untreated group at day ４ ( P ＜ ０ ．０５) ； and evidently larger than that in the YXSFC group and the untreated group at day １１ ( P ＜ ０ ．０５) ．The expression of VEGF in the hair follicle was distinctively higher than that in the YXSFC group and the untreated group at day １１ and day １７ ( P ＜ ０ ． ０５) ． Conclusion ： HXBSM upregulates VEGF expression to accelerate blood vessel neogenesis and hair growth ． · ０ ７ １ · 中西医 结合 学报 ２００７ 年 ３ 月 第 ５ 卷 第 ２ 期 Jo urn al o f C hinese In t eg ra tiv e M edicin e ， M arch ２００７ ； V ol ．５ ， N o ．２