Effects of humic acids on the recovery of different bacterial strains in an in vitro chicken digestive model

Maguey-González, Jesús Adonai; Gómez-Rosales, Sergio; Angeles, María de Lourdes; López-Hernández, Luis Humberto; Rodríguez-Hernández, Elba; Solís-Cruz, Bruno; Hernández-Patlán, Daniel; Merino-Guzmán, Rubén; Téllez–Isaías, Guillermo

doi:10.1016/j.rvsc.2022.01.004

Cited by 10 publications

(14 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HA can also improve nutrient assimilation, as probably occurred during the experiments as bacterial counts were increased. It is probably that in the simulated digestive system the rapid transit time through the digestive compartments, the addition of the buffering solutions and the presence of several dietary components in the intestine overcome the possible accumulation of toxic metabolites [96]. In two recent in vivo studies, increased lactic acid bacteria counts were reported in the gut of HS-added broilers [77,91].…”

Section: Digestive Microbiotamentioning

confidence: 99%

“…In two recent published articles, more descriptive effects of HS in poultry have been given [68,96]. In several experiments, enhanced final body weight and weight gain and reduced feed conversion ratio have been reported in HS-added broilers using different commercial sources of HS [24,68,113,114].…”

Section: Productive Response In Animals Supplemented With Humic Subst...mentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of Action of Humic Substances as Growth Promoters in Animals

Angeles¹,

Gómez-Rosales²,

Téllez–Isaías³

2022

Humus and Humic Substances - Recent Advances

View full text Add to dashboard Cite

A review of the latest research on the addition of humic substances (HSs) in the drinking water or feed of farm animals including poultry, pigs, dairy cows and calves, goats, and rabbits was carried out. The results reinforces the promoted protective action of HS on the digestive mucosa, their antioxidant properties, immunomodulatory, and anti-inflammatory attributes, the suggested microbial shift to a probiotic-type bacteria in the gut, including antiparasitic and antifungal effects, as well as their influence on the higher efficient of minerals, proteins, and lipids utilization within the body. The outstanding improvements in health, productivity, and meat and milk quality obtained from animals supplemented with HS are common features in the majority of available research. The main benefits in the gastrointestinal tract stem from the formation of protective barriers on the epithelial mucosa due to its colloidal properties and stimulation of mucin production. HS also promotes the development of probiotic microbiota and positive changes in bacterial fermentation patterns, which results in improved intestinal health and integrity. As a result, a cascade of benefits is derived within the body, reinforcing antioxidant protection responses, immunomodulation, and anti-inflammation mechanisms, as well as improving nutrient utilization efficiency. However, the truly molecular mechanisms of action of HS in the intestine and throughout the body remain unknown.

show abstract

Section: Digestive Microbiotamentioning

confidence: 99%

Section: Productive Response In Animals Supplemented With Humic Subst...mentioning

confidence: 99%

Mechanisms of Action of Humic Substances as Growth Promoters in Animals

Angeles¹,

Gómez-Rosales²,

Téllez–Isaías³

2022

Humus and Humic Substances - Recent Advances

View full text Add to dashboard Cite

show abstract

“…These groups are thought to be potential pollutant-binding sites, such as metallic species, herbicides, and pesticides [ 10 ], as well as complex formations with metal cations [ 11 ]. Recently, our research group studied an extract of HA from vermicompost under different experimental conditions to clarify its mechanism of action [ 12 , 13 , 14 ]. In previous in vitro and in vivo studies, several sources of HS containing mixtures of HA and FA have been used as mycotoxin binders [ 15 ]; however, purified HA from vermicompost has never been tested against AFB 1 .…”

Section: Introductionmentioning

confidence: 99%

Humic Acids Preparation, Characterization, and Their Potential Adsorption Capacity for Aflatoxin B1 in an In Vitro Poultry Digestive Model

Maguey-González

Nava-Ramírez

Gómez-Rosales

et al. 2023

Toxins

Self Cite

View full text Add to dashboard Cite

Vermicompost was used for humic acid (HA) preparation, and the adsorption of aflatoxin B1 (AFB1) was investigated. Two forms of HA were evaluated, natural HA and sodium-free HA (SFHA). As a reference, a non-commercial zeolitic material was employed. The adsorbents were characterized by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), energy-dispersive X-ray spectroscopy (EDS), zeta potential (ζ-potential), scanning electron microscopy (SEM), and point of zero charge (pHpzc). The adsorbent capacity of the materials when added to an AFB1-contaminated diet (100 µg AFB1/kg) was evaluated using an in vitro model that simulates the digestive tract of chickens. Characterization results revealed the primary functional groups in HA and SFHA were carboxyl and phenol. Furthermore, adsorbents have a highly negative ζ-potential at the three simulated pH values. Therefore, it appears the main influencing factors for AFB1 adsorption are electrostatic interactions and hydrogen bonding. Moreover, the bioavailability of AFB1 in the intestinal section was dramatically decreased when sorbents were added to the diet (0.2%, w/w). The highest AFB1 adsorption percentages using HA and SFHA were 97.6% and 99.7%, respectively. The zeolitic material had a considerable adsorption (81.5%). From these results, it can be concluded that HA and SFHA from vermicompost could be used as potential adsorbents to remove AFB1 from contaminated feeds.

show abstract

“…27 HAs are compounds formed by an aromatic and heterocyclic structure, soluble in alkaline medium, have high ion exchange capacity and can be isolated from nonrenewable carbon (C) sources such as peat, coal and leonardite. [28][29][30][31] HAs comprise phenolic, carboxylic acid, enolic, quinone, sugars, and peptides; however, the phenolic and carboxylic groups are more prevalent in HA structures. 29 Owing to their high natural availability and excellent properties, HA has attracted great interest in several areas, such as environment, [30][31][32] biology 33,34 and medicine.…”

Section: Introductionmentioning

confidence: 99%

“…[28][29][30][31] HAs comprise phenolic, carboxylic acid, enolic, quinone, sugars, and peptides; however, the phenolic and carboxylic groups are more prevalent in HA structures. 29 Owing to their high natural availability and excellent properties, HA has attracted great interest in several areas, such as environment, [30][31][32] biology 33,34 and medicine. 35,36 The classical processes to recover humic substances from organic matter involve extracting with solvents, such as formic acid and sodium pyrophosphate.…”

Section: Introductionmentioning

confidence: 99%

Partition of humic acids using alkaline aqueous two‐phase systems constituted of alcohol and hydroxides

Vieira,

Buarque,

Gomes

et al. 2024

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUNDHumic acids (HAs) are macromolecules classified within the group of humic substances that are mainly related to the agricultural and environmental sectors, but they have attracted interest from the medicine, pharmaceutical, and cosmetic industries. However, these latter sectors depend on developing extraction and purification technologies for adequate use. Thus, this paper aims to study the partitioning and recovery of HA using alkaline two‐phase aqueous systems (ATPSs) based on alkali metal‐based hydroxides and alcohols. Initially, the influence of alcohols (methanol, ethanol, 1‐propanol, and 2‐propanol) and hydroxides (NaOH, KOH, and LiOH) in the phase diagrams construction was addressed.RESULTSThe phase formation is correlated to an increase in the hydrophobicity of alcohols and hydroxides, which allows for the enlargement of the biphasic region. In the proposed systems, HA is always partitioned to the hydroxide‐rich phase. Increasing the hydrophobicity of the alcohol favors the migration of HA to the opposite phase, while increasing the hydrophilicity of the hydroxides leads to better partition and recovery values. It was observed that increasing TLL favors the recovery of HA. Furthermore, there was a decrease in alcohol concentration in the hydroxide‐rich phase with an increase in the alkyl chain and TLL, improving the recovery. Finally, the best reached‐recovery value was 98.18 ± 0.41% (KHA = 0.038 ± 0.02) for ATPS formed by 1‐propanol + NaOH with TLL ≈ 55 at 298 K.CONCLUSIONSThe alkaline systems can be a novel route for recovering humic substances from the environment.This article is protected by copyright. All rights reserved.

show abstract

Effects of humic acids on the recovery of different bacterial strains in an in vitro chicken digestive model

Cited by 10 publications

References 33 publications

Mechanisms of Action of Humic Substances as Growth Promoters in Animals

Mechanisms of Action of Humic Substances as Growth Promoters in Animals

Humic Acids Preparation, Characterization, and Their Potential Adsorption Capacity for Aflatoxin B1 in an In Vitro Poultry Digestive Model

Partition of humic acids using alkaline aqueous two‐phase systems constituted of alcohol and hydroxides

Contact Info

Product

Resources

About