Effects of human umbilical cord mesenchymal stem cells derived from exosomes on migration ability of endometrial glandular epithelial cells

Abstract

The present study aimed to investigate the effects of human umbilical cord mesenchymal stem cells (Huc-MScs)-derived exosomes on the migratory abilities of endometrial glandular epithelial cells, and to evaluate the underlying mechanism from the perspective of epithelial-mesenchymal transition (eMT). Huc-MScs were prepared from human umbilical cord, and eutopic endometrial glandular epithelial cells were isolated from patients with endometriosis. The exosomes derived from Huc-MScs (Huc-MScs-exo) were prepared using an exosome extraction kit. The endometrial glandular epithelial cells were randomly divided into two groups: Huc-MScs-exo and control. cell migratory ability was assessed and western blotting was used to detect the expression levels of eMT. The results of the present study demonstrated that Huc-MSCs-exo treatment significantly enhanced the migration of endometrial glandular epithelial cells from patients with endometriosis (P<0.05). The present study also demonstrated that treatment with Huc-MScs-exo inhibited the expression levels of e-cadherin and promoted the expression levels of Vimentin and n-cadherin at both the mrna and protein level. The results of the current study indicate that Huc-MScs-exo enhance the migratory ability of endometrial glandular epithelial cells via promotion of eMT.

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosome Repairs Endometrial Epithelial Cells Injury Induced by Hypoxia via Regulating miR-663a/CDKN2A Axis

“…Exosomes derived from hucMSCs can improve cell viability and exhibit anti-inflammatory properties in EECs induced by oxygen and glucose deprivation/reoxygenation (OGD/R) [ 30 ]. HucMSC-Ex enhances the migratory ability of endometrial glandular epithelial cells isolated from endometriosis patients via promotion of EMT [ 31 ]. Collagen scaffold/UCMSCs facilitates endometrial regeneration and fertility restoration, in addition, better collagen remodeling, obvious luminal structures, and thicker endometrium are noticed [ 10 , 32 ].…”

A promising future for endometriosis diagnosis and therapy: extracellular vesicles - a systematic review

“…Only validated 2 miRNAs via RT-qPCR. No description of UC methods Feng 2020 [ 24 ] Human ( n = 6) None CCM of primary umbilical cord derived MSCs Exosome kit (#E1310; Bioruo) Partially No investigation Not discussed Not discussed EVs from MSCs may enhance cell migration of endometrial cells Limited numbers. EV source and endometriosis tissue from different patients Harp 2016 [ 19 ] Human ( n = 5) Human ( n = 5) CCM of ESCs derived from endometrial biopsies and endometriosis lesion biopsies UC Yes miRNAs (seq).…”

Recent progress of Bioinspired Hydrogel-based delivery system for endometrial repair

“…Frontiers in Bioengineering and Biotechnology frontiersin.org characteristic of hydrogel and the ability to controlled-release its embedded molecule, encapsulation of exosomes with hydrogel provide an outstanding candidate in plenty of treatments including bone and cartilage regeneration, cardiovascular diseases, spinal cord injury, periodontal and corneal repairs (Xie et al, 2022). Exosome has immense potential of treating injured endometrium by regulating EMT (Yao et al, 2019;Feng et al, 2020), miRNA (Tan et al, 2020;Shi et al, 2021), proliferation (Lv et al, 2020) and endometrium angiogenesis (Zhang et al, 2022). However, all these effects are achieved via the suboptimal approach of direct injection which restricts the activity of exosomes by local tissue irritation or reduced self-life.…”