Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts

Oono, Takashi; Shirafuji, Yoshinori; Huh, Wook Kang; Akiyama, Hisanori; Iwatsuki, Keiji

doi:10.1007/s00403-002-0310-6

Cited by 46 publications

(38 citation statements)

References 21 publications

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“…Both neutrophil a-defensins and LL-37 have been shown to affect epithelial wound repair. Specifically, neutrophil defensins are mitogenic for fibroblasts [39] and increase collagen type I and III expression in both dermal and corneal fibroblasts [40,41], suggesting that increased levels of a-defensins may contribute to tissue fibrosis. Therefore, the higher levels of a-defensins in transplant recipients with BOS may be associated with an exaggerated repair response to airway damage.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptides in lung transplant recipients with bronchiolitis obliterans syndrome

Anderson

Hiemstra²,

Ward³

et al. 2008

European Respiratory Journal

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Mechanisms other than classical alloimmunity are implicated in the pathogenesis of bronchiolitis obliterans syndrome (BOS). It was hypothesised that antimicrobial peptides (AMPs), elements of the innate immune response, have a role in BOS pathogenesis.Pulmonary expression of the neutrophil-derived AMPs human cathelicidin (hCAP)-18/LL-37 and a-defensins (human neutrophil peptides (HNP) 1-3), and the epithelial cell-derived AMPs human b-defensin (hBD)-2, elafin and secretory leukoprotease inhibitor (SLPI) were measured in stable lung transplant recipients and those with BOS. The relationship between airway pathogens and AMP levels was examined.Bronchoalveolar lavage (BAL) was performed on 44 lung transplant recipients (30 stable, 14 with BOS). BAL was cultured for pathogens and ELISA for AMPs was performed. The presence of airway pathogens was associated with significantly increased levels of neutrophil-derived and epithelial-derived AMPs. When patients without pathogens in BAL fluid were analysed, eight recipients with BOS had elevated hCAP-18/LL-37 and HNP 1-3 compared with 25 stable recipients. hBD-2 and elafin levels were comparable in BOS and stable recipients, but SLPI levels were reduced in BOS.Bronchiolitis obliterans syndrome is associated with elevated airway human cathelicidin 18/ LL-37 and human neutrophil peptides 1-3 from activated neutrophils, even in the absence of pathogens. Together with reduced airway secretory leukoprotease inhibitor this may favour nonalloimmune airway injury with reduced antiprotease defence and increased neutrophil degranulation.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial peptides in lung transplant recipients with bronchiolitis obliterans syndrome

Anderson

Hiemstra²,

Ward³

et al. 2008

European Respiratory Journal

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“…Many of those cationic amphipathic molecules have immunomodulatory activities, modulating inflammation and enhancing protective responses, including phagocytic recruitment and deficiency, as well as angiogenesis and wound repair (11,12). Structurally, HDPs are highly diverse, and their primary sequence can be used as the templates for the development of synthetic peptides with optimal immunomodulatory and/or antimicrobial activity to influence future clinically relevant therapeutic guidelines (13).…”

mentioning

confidence: 99%

An Immunomodulatory Peptide Confers Protection in an Experimental Candidemia Murine Model

Freitas

Lima

Freire

et al. 2017

Antimicrob Agents Chemother

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Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida spp. are capable of breaching the epithelial barrier of immunocompromised patients with neutrophil and cell-mediated immune dysfunctions and can also disseminate to multiple organs through the bloodstream. Here we examined the action of innate defense regulator 1018 (IDR-1018), a 12-amino-acid-residue peptide derived from bovine bactenecin (Bac2A): IDR-1018 showed weak antifungal and antibiofilm activity against a Candida albicans laboratory strain (ATCC 10231) and a clinical isolate (CI) (MICs of 32 and 64 g · ml Ϫ1 , respectively), while 8-fold lower concentrations led to dissolution of the fungal cells from preformed biofilms. IDR-1018 at 128 g · ml Ϫ1 was not hemolytic when tested against murine red blood cells and also has not shown a cytotoxic effect on murine monocyte RAW 264.7 and primary murine macrophage cells at the tested concentrations. IDR-1018 modulated the cytokine profile during challenge of murine bone marrow-derived macrophages with heatkilled C. albicans (HKCA) antigens by increasing monocyte chemoattractant protein 1 (MCP-1) and interleukin-10 (IL-10) levels, while suppressing tumor necrosis factor alpha (TNF-␣), IL-1␤, IL-6, and IL-12 levels. Mice treated with IDR-1018 at 10 mg · kg Ϫ1 of body weight had an increased survival rate in the candidemia model compared with phosphate-buffered saline (PBS)-treated mice, together with a diminished kidney fungal burden. Thus, IDR-1018 was able to protect against murine experimental candidemia and has the potential as an adjunctive therapy.

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“…In contrast, the expression of matrix-metalloproteinase-1 was decreased. The authors suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition (153). Another study showed mitogenic activity of HNPs in epithelial and fibroblast cell lines in vitro (154).…”

Section: Host Defense Peptides In Woundsmentioning

confidence: 99%

“…Oono et al (153) showed that synthetic HNP-1 increases the expression of pro-collagen mRNA and protein in dermal fibroblast cultures. In contrast, the expression of matrix-metalloproteinase-1 was decreased.…”

Section: Host Defense Peptides In Woundsmentioning

confidence: 99%

Host Defense Peptides in Wound Healing

Steinstraesser

Koehler

Jacobsen

et al. 2008

Mol Med

143

112

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Host defense peptides are effector molecules of the innate immune system. They show broad antimicrobial action against gram-positive and -negative bacteria, and they likely play a key role in activating and mediating the innate as well as adaptive immune response in infection and inflammation. These features make them of high interest for wound healing research. Nonhealing and infected wounds are a major problem in patient care and health care spending. Increasing infection rates, growing bacterial resistance to common antibiotics, and the lack of effective therapeutic options for the treatment of problematic wounds emphasize the need for new approaches in therapy and pathophysiologic understanding. This review focuses on the current knowledge of host defense peptides affecting wound healing and infection. We discuss the current data and highlight the potential future developments in this field of research.

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Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts

Cited by 46 publications

References 21 publications

Antimicrobial peptides in lung transplant recipients with bronchiolitis obliterans syndrome

Antimicrobial peptides in lung transplant recipients with bronchiolitis obliterans syndrome

An Immunomodulatory Peptide Confers Protection in an Experimental Candidemia Murine Model

Host Defense Peptides in Wound Healing

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