Effects of human chorionic gonadotrophin, oestradiol and estromustine on testicular blood flow in hypophysectomized rats

Daehlin, L.; Damber, Jan‐Erik; Selstam, Gunnar; Bergman, Bo

doi:10.1111/j.1365-2605.1985.tb00818.x

Cited by 13 publications

(5 citation statements)

References 22 publications

(23 reference statements)

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“…Setchell & Sharpe (1981) described an increase in testicular blood flow 16 h after injection of 2001U human chorionic gonadotrophin (hCG). An increase in testi¬ cular blood flow was also found 24 h after the admin¬ istration of various doses of hCG (Punjabi, van Hoecke, Verdonck & Vermeulen, 1984) or after repeated injections of low doses of hCG (Daehlin, Damber, Selstam & Bergman, 1985). Damber, Bergh & Daehlin (1985) reported an increase in testicular blood flow 8 h after administration of hCG, while testis blood flow was found to be decreased 2 h after hCG treatment (Wang, Gu, Qian & Jing, 1984).…”

Section: Introductionmentioning

confidence: 79%

Reduction of testicular blood flow and focal degeneration of tissue in the rat after administration of human chorionic gonadotrophin

Vliet

Rommerts²,

Rooij³

et al. 1988

Journal of Endocrinology

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The effect of 100 IU human chorionic gonadotrophin (hCG) on testicular capillary blood flow was studied in adult male rats using a 133Xe clearance method and a radioactive microsphere technique. To investigate the role of Leydig cells in regulation of testicular blood flow after treatment with hCG, rats were pretreated with ethane dimethylsulphonate (EDS) which selectively destroys mature Leydig cells. Six hours after treatment with hCG, testicular blood flow decreased in control and hypophysectomized rats to 25-50% of normal values, but not in EDS-pretreated animals. Prostaglandin E2 levels were also determined 6 h after an injection of hCG. A 300-fold increase in the concentration of prostaglandin E2 occurred in normal testis tissue. This rise was markedly inhibited if EDS was given 3 days before administration of hCG. Furthermore, 6 h after administration of hCG, the filling of the testicular capillary bed with methylacrylate was decreased, while in control rats and rats treated with EDS and hCG, complete filling of the capillaries was seen. Cell degeneration in some subcapsular seminiferous tubules was observed 6-10 days after treatment with hCG. The results suggest that the hCG-induced precapillary vasoconstriction, probably mediated (in part) by prostaglandins, causes reduction in testicular blood flow 6 h after administration of hCG, and may result in cell damage.

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Section: Introductionmentioning

confidence: 79%

Reduction of testicular blood flow and focal degeneration of tissue in the rat after administration of human chorionic gonadotrophin

Vliet

Rommerts²,

Rooij³

et al. 1988

Journal of Endocrinology

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“…The rat was placed in a supine position on a heating pad. Catheters were placed in the internal carotid artery and tail artery to be used for blood flow measurements using the radioactive microsphere method (Damber & Janson, 1977;Daehlin et al, 1985) using microspheres purchased from New England Nuclear Products (Boston, U.S.A.) (I4'Ce, 7.53 mCi g-'; 16.6 k 0.2 pm diameter). Mean arterial pressure was registered countinuously during measurement of testicular microcirculation using a laser Doppler flowmeter in which the output signal from the laser Doppler flowmeter is related linearly to the number of red cells multiplied by their velocity (Tenland, 1982;Damber et al, 1986).…”

Section: Methodsmentioning

confidence: 99%

Arginine‐vasopressin induced changes in testicular blood flow

Widmark¹,

Damber²,

Bergh

1991

Int J of Andrology

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As arginine-vasopressin (AVP) has been suggested as a possible paracrine regulator in the testis, the present study was designed to evaluate the effects of AVP on testicular blood flow and vascular permeability. Intratesticular injection of AVP caused a dose-dependent decrease in total testicular blood flow, with 1 ng AVP being the lowest dose that induced a decrease. The AVP-induced decrease in blood flow was blocked completely by treatment with an AVP antagonist, but the latter had no effect on its own. High doses of AVP (100 and 1000 ng) decreased testicular IF volume and slightly increased the IF testosterone concentration. At 10 min and 1 h after intratesticular injection of 100 ng AVP testicular blood flow was decreased, followed by an increase at 6 h; testosterone concentrations were increased at both 10 min and 6 h after this treatment. The microcirculatory blood flow pattern (vasomotion) was not influenced by AVP. The present study shows that AVP in low doses influences testicular blood flow rather selectively because AVP was without any major effect on vasomotion, interstitial fluid volume and testosterone production. AVP might therefore be of importance in the regulation of testicular blood flow.

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“…Testicular blood flow was measured using the radioactive microsphere method as described previously in detail (Damber & Janson, 1977;Daehlin et al, 1985). The microspheres (141 cerium, 16.5 k 0.1 pg in diameter) were purchased from New England Nuclear Products (Boston, MA).…”

Section: Testicular Blood Flowmentioning

confidence: 99%

Does follicle‐stimulating hormone or pregnant mare serum gonadotrophin influence testicular blood flow in rats?

Bergh¹,

Damber²,

Lieu³

et al. 1992

Int J of Andrology

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Treatment of adult rats with 25 iu follicle-stimulating hormone (FSH) did not influence testicular blood flow, serum testosterone, vasomotion or intravascular leucocyte concentration at 6, 12 and 24 h after treatment. Treatment of adult rats with 50 iu pregnant mare serum gonadotrophin (PMSG) resulted in a two-fold increase in testicular blood flow at 24 h, and an increase in interstitial fluid volume at 36 h after treatment. This PMSG-induced increase in blood flow did not occur in Leydig cell-depleted animals, suggesting that the effect on blood flow is mediated via the Leydig cells. PMSG injection stimulated testosterone secretion but it did not influence vasomotion, and it only marginally increased the secretion of leucotactic factors in the testis. The present study suggests that FSH has no apparent effects on testicular blood flow and that the effects of PMSG (a hormone with both FSH and LH-like activity) is mediated via stimulation of the Leydig cells.

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Effects of human chorionic gonadotrophin, oestradiol and estromustine on testicular blood flow in hypophysectomized rats

Cited by 13 publications

References 22 publications

Reduction of testicular blood flow and focal degeneration of tissue in the rat after administration of human chorionic gonadotrophin

Reduction of testicular blood flow and focal degeneration of tissue in the rat after administration of human chorionic gonadotrophin

Arginine‐vasopressin induced changes in testicular blood flow

Does follicle‐stimulating hormone or pregnant mare serum gonadotrophin influence testicular blood flow in rats?

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