Effects of HSP70 on the compression force-induced TNF-α and RANKL expression in human periodontal ligament cells

Mitsuhashi, Masami; Yamaguchi, Masaru; Kojima, Tadashi; Nakajima, Ryo; Kasai, Kazuhiko

doi:10.1007/s00011-010-0253-x

Cited by 30 publications

(19 citation statements)

References 41 publications

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“…These data indicate that marsupialization may affect the OPG/RANK/RANKL signaling pathway. Considering that the high pressure within the cyst cavity may induce RANKL expression and result in the formation of osteoclasts, we speculated that the effect of marsupialization on the OPG/RANK/RANKL signaling pathway may be due to the release of intracystic pressure (26,27). In addition, marsupialization might induce inflammation and eliminate the possibly hypoxic environment of the cavity (8,28), affecting activation of the OPG/RANK/RANKL signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

Zhao

Man

et al. 2017

Journal of Oral Science

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Bone resorption in the jaws is one of the most severe complications of keratocystic odontogenic tumors (KCOTs), and can be treated by either enucleation or marsupialization. However, the effects of marsupialization on bone regeneration adjacent to KCOTs, and the mechanisms involved, are still unclear. In this study, samples of 27 KCOTs were collected (20 before marsupialization and 7 after marsupialization) to detect the expression of bone regeneration-related molecules adjacent to KCOTs by immunohistochemistry and real-time quantitative polymerase chain reaction (qPCR). The results showed that bone formation was significantly enhanced in the KCOT capsule wall adjacent to bone after marsupialization, as demonstrated by alkaline phosphatase activity assay and immunostaining for bone morphogenetic protein. Moreover, immunohistochemistry revealed that osteoprotegerin (OPG) was up-regulated in the KCOT capsule wall adjacent to bone after marsupialization, while receptor activator of nuclear factor-κB ligand (RANKL) was down-regulated. Real-time qPCR also demonstrated increased expression of OPG after marsupialization, accompanied by a decrease in the expression of osteoclastogenesis-related molecules such as cathepsin K, matrix metalloproteinase-9, NFATc1, RANK and RANKL. This study provides further evidence that marsupialization may promote bone regeneration adjacent to KCOTs partially through regulation of the OPG/RANK/RANKL signaling pathway.

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Section: Discussionmentioning

confidence: 99%

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

Zhao

Man

et al. 2017

Journal of Oral Science

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“…Caspase 9 is the mediator of the intrinsic pathway, and an alteration in oxidative phosphorylation, hypoxia and oxidative stress can induce the activation of caspase 9 12) . We previously studied the mRNA expression of TNF-and found that it increased significantly 21) . We therefore hypothesize that the mechanism of apoptosis through caspase 8 activation is mediated by TNF-.…”

Section: Discussionmentioning

confidence: 99%

“…We previously reported the increased expressions of cytokines and chemokines during experimental tooth movement because of the application of heavy force (50 g) in vivo and the effects of compression forces (0-4.0 g/cm 2 ) in vitro 17,21) . The highest amount of cell damage was 16.9% in the compression force (4.0 g) group 21) . It is difficult to determine the optimal force during therapeutic orthodontic tooth movement, because periodontal tissue reactions vary among individuals.…”

Section: Discussionmentioning

confidence: 99%

Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Funakoshi

Yamaguchi

Asano

et al. 2013

J. Hard Tissue Biology.

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The effect of compression force on periodontal ligament (PDL) plays a critical role in orthodontic tooth movement. However, little is known about this effect on apoptosis and the underlying reactive mechanisms in PDL cells. This study focuses on the application of compression force associated with reactive oxygen species (ROS)-induced apoptosis in PDL cells. Following the application of orthodontic force to maxillary first molars, the molars were investigated using immunohistochemical analysis. The cell cycle and apoptosis were assessed in compression force-treated PDL cells using flow cytometry. Furthermore, we assayed the effect on ROS generation by DCFDA fluorescence. In vivo study revealed an increased number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and caspase 8-positive cells in the orthodontic force group. In our in vitro study model, the compression force increased the G1 phase and apoptotic cells. A large increase in the intracellular ROS levels in human (h) PDL cells was observed in the compression force group. Taken together, these results provide new information on compression force-induced G1 arrest and apoptosis in hPDL cells.

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“…This difference might be caused by the up-regulation of VEGF in that it could partially act as a macrophage colony-stimulating factor, 21 and the RANKL expression in OBs induced by COX-2(PGE 2 )/IL-1. 26,27 However, compression and hypoxia act in a magnitude-dependent manner, 29,30 while the adopted interventions in the present study (compression of 25 g/cm 2 and 2% O 2 ) may not be proportional to the in vivo environment, which should be further clarified.…”

Section: Discussionmentioning

confidence: 81%

Compression and hypoxia play independent roles while having combinative effects in the osteoclastogenesis induced by periodontal ligament cells

Yang

et al. 2015

The Angle Orthodontist

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Objective: To investigate the isolated and combined effects of compression and hypoxia on the osteoclastogenesis induced by periodontal ligament cells (PDLCs). Materials and Methods: A periodontal ligament tissue model (PDLtm) was established by 3-D culturing human PDLCs on a thin sheet of poly lactic-co-glycolic acid scaffold. The PDLtm was treated with hypoxia and/or compression for 6, 24, or 72 hours. After that, a real-time polymerase chain reaction was used for gene expression analysis. The conditioned media were used for the coculture of osteoblast and osteoclast (OC) precursors; tartrate-resistant acid phosphatase staining was done to examine OC formation. Results: Either compression or hypoxia alone significantly up-regulated the gene expression of pro-osteoclastogenic cytokines in the PDLtm and enhanced osteoclastogenesis in the cocultures, and the combination of the two had significantly stronger effects than either stimulation alone. In addition, comparing the two stimulants, we found that the osteoclastogenic property of the PDLCs peaked earlier (at 6 hours) in the compression group than in the hypoxia group (at 24 hours). Conclusions: Both compressive force and hypoxia may take part in initiating osteoclastogenesis in orthodontic tooth movement and may have combinatory effects, which could update our concepts of the mechanisms involved in the initiation of bone resorption on the pressure side of the tooth in question. (Angle Orthod. 2016;86:66-73.)

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Effects of HSP70 on the compression force-induced TNF-α and RANKL expression in human periodontal ligament cells

Abstract: These results indicate that HSP70 may modulate the mRNA expression of TNF-α and RANKL in hPDL cells in response to CF.

Cited by 30 publications

References 41 publications

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

Effect of Compression Force on Apoptosis in Human Periodontal Ligament Cells

Compression and hypoxia play independent roles while having combinative effects in the osteoclastogenesis induced by periodontal ligament cells

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