Effects of Hormone Treatments on Cut Flower Opening and Senescence in Wintersweet (Chimonanthus praecox)

Sui, Shunzhao; Luo, Jianghui; Liu, Daofeng; Ma, Jing; Men, Weiting; Fan, Lu; Bai, Yu; Li, Mingyang

doi:10.21273/hortsci.50.9.1365

Cited by 14 publications

(9 citation statements)

References 30 publications

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“…Additionally, for ornamental plants and cut flowers, the research on senescence is always one of the hot issues, which is closely related to its commercial value and market competitiveness [6]. In recent years, Sui et al [7] reported the effects of hormones on the wintersweet cut flower senescence. However, little information is available regarding the molecular mechanisms of wintersweet senescence.…”

Section: Introductionmentioning

confidence: 99%

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

Huang¹,

Liu²,

Huang³

et al. 2019

IJMS

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The WRKY transcription factors are one of the most important plant-specific transcription factors and play vital roles in various biological processes. However, the functions of WRKY genes in wintersweet (Chimonanthus praecox) are still unknown. In this report, a group IIc WRKY gene, CpWRKY71, was isolated from wintersweet. CpWRKY71 was localized to the nucleus and possessed transcriptional activation activity. qRT-PCR (quantitative real-time PCR) analysis showed that CpWRKY71 was expressed in all tissues tested, with higher expression in flowers and senescing leaves. During the flower development, the highest expression was detected in the early-withering stage, an obvious expression of CpWRKY71 was also observed in the flower primordia differentiation and the bloom stage. Meanwhile, the expression of CpWRKY71 was influenced by various abiotic stress and hormone treatments. The expression patterns of the CpWRKY71 gene were further confirmed in CpWRKY71pro:GUS (β-glucuronidase) plants. Heterologous overexpression of CpWRKY71 in Arabidopsis caused early flowering. Consistent with the early flowering phenotype, the expression of floral pathway integrators and floral meristem identity (FMI) genes were significantly up-regulated in transgenic plants. In addition, we also observed that the transgenic plants of CpWRKY71 exhibited precocious leaf senescence. In conclusion, our results suggested that CpWRKY71 may be involved in the regulation of flowering and leaf senescence in Arabidopsis. Our study provides a foundation for further characterization of CpWRKY genes function in wintersweet, and also enrich our knowledge of molecular mechanism about flowering and senescence in wintersweet.

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Section: Introductionmentioning

confidence: 99%

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

Huang¹,

Liu²,

Huang³

et al. 2019

IJMS

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“…These studies have revealed that exogenous application of cytokinins improve the postharvest life of various cut flowers like Lilium, Dianthus, Nelumbo, Gerbera, Iris, Hemerocallis (Macnish et al 2010a;Imsabai and van Doorn 2013;Reid and Wu 2018). The role of endogenous and exogenously applied cytokinins has been studied at transgenic level in some flowers like Petunia, Nicotiana, Chimonanthus, Rosa and Dianthus (Mor et al 1983;Trivellini et al 2014;Sui et al 2015). Although both classes of cytokinins (adenine type and phenyl urea derivatives) proved effective in improving postharvest performance of cut flowers, yet the effect of thidiazuron was more promising.…”

Section: Introductionmentioning

confidence: 99%

Adenine type and diphenyl urea derived cytokinins improve the postharvest performance of Iris germanica L. cut scapes

Ahmad

Tahir

Wani

et al. 2018

Physiol Mol Biol Plants

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An experiment was designed to evaluate the effect of various adenine derived cytokinins (kinetin and 6-benzylaminopurine) and diphenyl urea cytokinin (thidiazuron) on the postharvest performance of cut scapes of Iris germanica. Flower scapes were harvested with the oldest bud at '1 day before anthesis stage', brought to laboratory under water, cut to a uniform length of 35 cm, divided into three sets viz., kinetin (KIN), 6-benzyl aminopurine (BAP) and thidiazuron (TDZ). Each set of scapes was treated with a particular cytokinin alone or in combination with 0.1 M sucrose. TDZ was effective than KIN and BAP in improving the postharvest life of the I. germanica scapes by 5.4 days as compared to the control (untreated scapes held in distilled water). This was because of the minimum percentage of bud abortion by TDZ application. Cytokinin application resulted in increased antioxidant activity, higher protein and phenolic content, besides a decrease in specific protease activity and a-amino acids in the tepal tissues. Application of TDZ resulted in the maximum increase in the superoxide dismutase, catalase and ascorbate peroxidase activity in the tepal tissues. The scapes treated with BAP and KIN maintained higher carbohydrate content in the tissue samples as compared to control and TDZ treated scapes. TDZ and BAP application resulted in increased membrane stability because of the decreased lipoxygenase activity which prevented membrane lipid peroxidation. Among the cytokinins tested, TDZ proved to be the promising cytokinin in improving the postharvest performance of beautiful flowers of I. germanica scapes.

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“…The contraction of an SRG1 annotated gene family in J. hindsii could be related to phenology differences. The abscisic acid (ABA) and ethylene induced (Sui et al 2015) zinc-finger transcription factor, SRG1 has been implicated in leaf senescence in Arabidopsis (Callard et al 1996, Cui et al 2018) and fruit ripening in strawberry (Chen et al 2016). Discovering additional contractions (and expansions) in phenology-related gene families between closely related species might set a natural experiment for establishing the genetic signatures correlated with species susceptible to climate change-associated fitness loss.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Six Juglans Species Reveals Patterns of Disease-associated Gene Family Contractions

Trouern-Trend

Falk

Zaman

et al. 2019

Preprint

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Juglans (walnuts), the most speciose genus in the walnut family (Juglandaceae) represents most of the family’s commercially valuable fruit and wood-producing trees and includes several species used as rootstock in agriculture for their resistance to various abiotic and biotic stressors. We present the full structural and functional genome annotations of six Juglans species and one outgroup within Juglandaceae (Juglans regia, J. cathayensis, J. hindsii, J. microcarpa, J. nigra, J. sigillata and Pterocarya stenoptera) produced using BRAKER2 semi-unsupervised gene prediction pipeline and additional in-house developed tools. For each annotation, gene predictors were trained using 19 tissue-specific J. regia transcriptomes aligned to the genomes. Additional functional evidence and filters were applied to multiexonic and monoexonic putative genes to yield between 27,000 and 44,000 high-confidence gene models per species. Comparison of gene models to the BUSCO embryophyta dataset suggested that, on average, genome annotation completeness was 89.6%. We utilized these high quality annotations to assess gene family evolution within Juglans and among Juglans and selected Eurosid species, which revealed significant contractions in several gene families in J. hindsii including disease resistance-related Wall-associated Kinase (WAK) and Catharanthus roseus Receptor-like Kinase (CrRLK1L) and others involved in abiotic stress response. Finally, we confirmed an ancient whole genome duplication that took place in a common ancestor of Juglandaceae using site substitution comparative analysis.SIGNIFICANCEHigh-quality full genome annotations for six species of walnut (Juglans) and a wingnut (Pterocarya) outgroup were constructed using semi-unsupervised gene prediction followed by gene model filtering and functional characterization. These annotations represent the most comprehensive set for any hardwood genus to date. Comparative analyses based on the gene models uncovered rapid evolution in multiple gene families related to disease-response and a whole genome duplication in a Juglandaceae common ancestor.

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Effects of Hormone Treatments on Cut Flower Opening and Senescence in Wintersweet (Chimonanthus praecox)

Cited by 14 publications

References 30 publications

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

CpWRKY71, a WRKY Transcription Factor Gene of Wintersweet (Chimonanthus praecox), Promotes Flowering and Leaf Senescence in Arabidopsis

Adenine type and diphenyl urea derived cytokinins improve the postharvest performance of Iris germanica L. cut scapes

Comparative Genomics of Six Juglans Species Reveals Patterns of Disease-associated Gene Family Contractions

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