Effects of Histone Deacetylase Inhibitors on the Early Development of Bovine Androgenetic Embryos

Abstract

Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In our previous study, we enhanced sperm chromatin remodeling within the bovine sperm injection-derived androgenentic (SpI-AG) embryos by sperm pretreatment, and thereby improved their early developmental competence. In this study, we found that blastocyst development of SpI-AG embryos could be elevated by the histone deacetylase inhibitor (HDACi). First, we optimized the efficacy of two histone deacetylase inhibitors [trichostatin A (TSA) and Scriptaid (SCR)] in a dose (0, 5, 10, 20, 50, and 100 nM for TSA; 0, 50, 100, 200, 300, and 500 nM for SCR, respectively) and time-dependent (0, 10, 15, 20, and 25 h) manner on the developmental capacity of these embryos. Furthermore, we quantitatively assessed the alterations in histone H3 and H4 overall acetylation levels and blastocyst quality of SpI-AG embryos by immunofluorescence staining. We found a significantly improved morula and blastocyst development rate of SpI-AG embryos at a mild dose of TSA (20 nM) or SCR (200 nM) for 15 h after embryo activation. Furthermore, both HDACi noticeably increased the levels of acetylated histone H3 and H4 in SpI-AG blastocyst embryos, whereas, SCR treatment improved the quality of blastocysts when compared with control group. In conclusion, HDACi is beneficial for early development of bovine SpI-AG embryos and can be used to improve the efficiency of its in vitro production.

The effects of melatonin on bovine uniparental embryos developmentin vitroand the hormone secretion of COCs

“…As for the uniparental embryos, the efficiency of developing to blastocyst is very low, especially the androgenetic embryo (Zhao et al, 2010; Xiao et al, 2013). Recently, the androgenetic embryos are produced through removing the maternal chromosomes, then injecting two spermatozoa into an oocyte (Vichera et al, 2011; Miki et al, 2009; Liang et al, 2009; Xiao et al, 2013; Zhang et al, 2014). Mammalian parthenogenetic embryos are obtained easily and efficiently, which have only oocyte-derived maternal genomes, and the androgenetic embryos that have only sperm-derived paternal genomes are produced difficultly (Sembon et al, 2012; Zhang et al, 2014).…”

“…Recently, the androgenetic embryos are produced through removing the maternal chromosomes, then injecting two spermatozoa into an oocyte (Vichera et al, 2011; Miki et al, 2009; Liang et al, 2009; Xiao et al, 2013; Zhang et al, 2014). Mammalian parthenogenetic embryos are obtained easily and efficiently, which have only oocyte-derived maternal genomes, and the androgenetic embryos that have only sperm-derived paternal genomes are produced difficultly (Sembon et al, 2012; Zhang et al, 2014). The early development of parthenogenetic and androgenetic embryos are limited, and arrest in the first 10 days and 8.5 days after gestation in mouse, respectively (Narasimha, Barton & Surani, 1997; Kono, 2006; Versieren et al, 2010).…”

“…The early development of parthenogenetic and androgenetic embryos are limited, and arrest in the first 10 days and 8.5 days after gestation in mouse, respectively (Narasimha, Barton & Surani, 1997; Kono, 2006; Versieren et al, 2010). Moreover, our previous studies showed that the treatments of sperm capacitation and removal of the acrosome and plasma membrane prior to sperm injection and the histone deacetylase inhibitor were beneficial for early development of bovine androgenetic embryos, whereas the blastocyst formation rate was still low (Xiao et al, 2013; Zhang et al, 2014). …”

“…Uniparental embryos are an effective tool to explore genetic effects on the process of maternal and paternal genomic imprinting, as well as the contribution of the maternal and paternal genome in early embryonic development (Latham, Kutyna & Wang, 1999; Kono et al, 2004; Gebert et al, 2009; Sepulveda-Rincon et al, 2016; Ogawa et al, 2009). Some studies report that the addition of granulocyte colony-stimulating factor and valproic acid, AY9944A-7 and histone deacetylase inhibitor in the IVM medium improve the viability of parthenogenetic embryos in porcine (Cai et al, 2015; Huang et al, 2015), sheep (Hao et al, 2015) and androgenetic embryos in bovine (Zhang et al, 2014), respectively. Adding melatonin to the IVM medium could improve the development of parthenogenetic embryos in porcine (Kang et al, 2009).…”

“…Parthenogenetic embryos are produced through incorporating oocyte-derived maternal genomes. Therefore, the androgenetic and parthenogenetic embryos are a valuable resource for providing an insight into the epigenetic reprogramming events and investigating the contribution of the paternal or maternal genome to early embryonic development during gametogenesis and embryogenesis (Obata et al, 2000; Ogawa et al, 2009; Xiao et al, 2014; Zhang et al, 2014). Moreover, parthenogenetic and androgenetic embryonic stem cells derived from the inner cell mass of blastocysts are histocompatible and offer an important autologous source of pluripotent stem cells that can be used for therapeutical transplantation (Zhao et al, 2010).…”