Effects of high oleic acid soybean on seed yield, protein and oil contents, and seed germination revealed by near‐isogeneic lines

Abstract

Typical soybean oil is composed of palmitic, stearic, oleic, linoleic and linolenic acids. High oleic acid content in soybean seed is a key compositional trait that improves oxidative stability and increases oil functionality and shelf life. Using a marker‐assisted selection method, near‐isogenic lines (NILs) of G00‐3213 for the high oleic trait were developed and yield tested. These NILs have various combinations of FAD2‐1A and FAD2‐1B alleles that were derived from the same backcrossing populations. The results indicated that G00‐3213 NILs with both homozygous mutant FAD2‐1A and FAD2‐1B alleles produced an average of 788 g/kg oleic acid content. The results also demonstrated that possessing these mutant alleles did not cause a yield reduction. Furthermore, seed germination tests across 12 temperatures (12.8–32.0°C) showed that modified seed composition for oleic acid in general did not have a major impact on seed germination. However, there was a possible reduction in seed germination vigour when high oleic seeds are planted in cold soil. The mutant FAD2‐1A and FAD2‐1B alleles did not hinder either seed or plant development.

Natural Variation in Fatty Acid Composition of Diverse World Soybean Germplasms Grown in China

“…Despite these benefits of LNA, polyunsaturated fatty acids, linoleic acid (LA), and LNA, adversely affect the oxidative stability of soybean oil, causing off-flavor problems [10]. Soybean oils with high OA (more than 80%) and low LNA (less than 1%) have been reported in several studies [11][12][13], and the monounsaturated fatty acid, OA, not only increases oil oxidative stability, but also reduces the production of trans-fat during food processing, making it more desirable.…”

TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

“…TALEN and CRISPR technologies were recently used to create targeted mutations based on GmFAD2-1A/GmFAD2-1B genes. The available high oleic acid soybeans based on GmFAD2-1A/GmFAD2-1B alleles (plastidial/ER fatty acid production) present affected germination in cold soil [ 13 ]. Loss of function of the GmFAD2-1A and GmFAD2-1B may affect the incorporation of fatty acids into phospholipids in the endoplasmic reticulum impacting membrane lipids and membrane fluidity; therefore, affecting cold stress tolerance and fatty acid stability of these lines [ 66 ].…”

“…Mutations within the GmFAD2-1A and GmFAD2-1B genes encoding fatty acid desaturases (omega-6 FAD; EC 1.3.1.35) in soybeans was used to produce high oleic acid soybean germplasm [ 8 , 10 ]. However, it has been reported that high seed oleic acid derived from GmFAD2-1 may have a possible reduction in seed germination when planted in cold soil [ 5 , 11 , 12 , 13 ]. The soybean community and industry are making tremendous efforts to determine the optimum allele combinations to produce environmentally stable high oleic/low linolenic acid soybean varieties for US soybean production environments (USB# 1720-162-0109).…”

Allelopathic Effect of Castanopsis Kawakamii Forest Litter on Seed Germination of Small Philippine Acacia (Acacia Confusa)

“…We speculate that this concentration is the optimum concentration of seed germination, and the beneficial components in the allelochemicals accumulate the most. Within this concentration range, the ion transporters in the cell and various ion channel proteins are involved in cell ion homeostasis, increasing seed germination rate and germination speed (Steven et al, 2003;Zhang et al, 2016;Bachleda et al, 2017;Kilic et al, 2016). The 1:320 litter extract concentration also promoted the germination of seeds, but the overall effect was not significant.…”