Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle

Rinnankoski‐Tuikka, Rita; Silvennoinen, Mika; Torvinen, Sira; Hulmi, Juha J.; Lehti, Maarit; Kivelä, Riikka; Reunanen, Hilkka; Kainulainen, Heikki

doi:10.1186/1743-7075-9-53

Cited by 40 publications

(36 citation statements)

References 66 publications

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“…Physiologic increase in PGC-1α expression in particular can protect against muscle wasting during increased metabolic demand or in pathologic conditions [38]. The PGC1α protein data are in agreement with a published report indicating that exercise does not potentiate PGC-1α expression in high fat diet fed animals [39]. It is possible that in the setting of demand ischemia and DIO, there is a repressive post-transcriptional mechanism of PGC1α protein synthesis or stabilization.…”

Section: Discussionsupporting

confidence: 84%

Revascularization and muscle adaptation to limb demand ischemia in diet-induced obese mice

Albadawi

Tzika

Rask‐Madsen

et al. 2016

Journal of Surgical Research

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Background Obesity and type 2 diabetes are major risk factors for peripheral arterial disease (PAD) in humans which can result in lower limb demand ischemia and exercise intolerance. Exercise triggers skeletal muscle adaptation including increased vasculogenesis. The goal of this study was to determine whether demand ischemia modulates revascularization, fiber size, and signaling pathways in the ischemic hind limb muscles of mice with diet-induced obesity (DIO). Materials and Methods DIO mice (n=7) underwent unilateral femoral artery ligation (FAL) and recovered for 2-weeks followed by 4-weeks with daily treadmill exercise to induce demand ischemia. A parallel sedentary ischemia group (n=7) had FAL without exercise. The contralateral limb muscles of sedentary ischemia served as control. Muscles were examined for capillary density, myofiber cross-sectional area (CSA), cytokine levels, and phosphorylation of STAT3 and ERK1/2. Results Exercise significantly enhanced capillary density (p<0.01) and markedly lowered CSA (p<0.001) in demand ischemia compared to sedentary ischemia. These findings coincided with a significant increase in G-CSF (p<0.001) and IL-7 (p<0.01) levels. In addition, phosphorylation of STAT3 and ERK1/2 (p<0.01) were increased while UCP-1 and MCP-1 protein levels were lower (p<0.05) without altering VEGF and TNFα protein levels. Demand ischemia increased the PGC1α mRNA (p<0.001) without augmenting PGC1α protein levels. Conclusions Exercise induced limb demands ischemia in the setting of DIO causes myofiber atrophy despite an increase in muscle capillary density. The combination of persistent increase in TNFα, lower VEGF and failure to increase PGC1α protein may reflect a deficient adaption to demand ischemia in DIO.

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Section: Discussionsupporting

confidence: 84%

Revascularization and muscle adaptation to limb demand ischemia in diet-induced obese mice

Albadawi

Tzika

Rask‐Madsen

et al. 2016

Journal of Surgical Research

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“…All histological images were quantified using ImageJ software version 1.47v. Histological images of SDH, Oil Red O, and PAS stained sections were analyzed as the area of expression according to the modified method of Rinnankoski-Tuikka et al [22]. A minimum of four captured images were analyzed for each staining.…”

Section: Methodsmentioning

confidence: 99%

Modulating effects of exercise training regimen on skeletal muscle properties in female polo ponies

et al. 2016

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BackgroundThe match play patterns in equestrian polo are unique and require specific training programs to ensure sport performance. The effect of commonly used exercise training regimens on the adaptation of skeletal muscle is unclear. The present study investigated the modulating effects of the classic training regimen, comprised of aerobic exercise training with increasing exercise intensities and varying duration combined with match play, on the properties of muscle in polo ponies. Nine healthy adult female polo ponies were subjected to four consecutive subsets of 1 year classic training regimen including basal activity (B), low intensity (L), low to moderate intensity (LM), and low to moderate intensity training plus match play during polo tournament (LMP), respectively. At the end of each training period, gluteus medius muscle samples were taken for determination of muscle fiber type distribution, muscle metabolic capacity, capillary density, and lipid and glycogen content. The expression profile of metabolic genes including succinate dehydrogenase (SDH), phosphofructokinase (PFK), glycogen phosphorylase (PYG), and glycogen synthase (GYS) were also measured.ResultsAmong all exercise training subsets, only LMP exercise period caused an increase in the number of oxidative fibers (type IIa), along with increases in properties related to oxidative metabolism including high capillary density, intramuscular lipid content, and expression of SDH and PYG genes, with a corresponding decrease in the number of type IIx muscle fibers.ConclusionThe combination of low to moderate and high intensity training in LMP are only sufficient to induce changes in oxidative characteristics. As the first scientific evidence providing such insight about the classic polo training regimen, the data forms a basis for further consideration in training program design.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0874-6) contains supplementary material, which is available to authorized users.

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“…In such studies, mice are fed a high-fat diet (HFD) to recapitulate obesity, hypercholesterolemia, and glucose intolerance [13]. After consuming an HFD for a period as short as four weeks, C57Bl/6J mice recapitulate many of the oocyte defects observed in obese women, including abnormal architecture of the Meiosis II spindle [14], distorted mitochondrial distribution [14], altered mitochondrial membrane potential[15], and abnormal mitochondrial morphology by electron microscopy [14].…”

Section: Introductionmentioning

confidence: 99%

Adverse effects of obesity and/or high-fat diet on oocyte quality and metabolism are not reversible with resumption of regular diet in mice

Reynolds

Boudoures

M.‐Y.

et al. 2015

Reprod. Fertil. Dev.

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Obesity, which affects over one-third of reproductive-age women, has negative effects on reproduction and results in oocyte defects in both mice and humans. In this study, we used a mouse model to examine whether the adverse effects of an obesogenic diet, specifically abnormal oocyte spindle formation, mitochondrial metabolism, and lipid accumulation, can be reversed by return to normal weight and metabolic profile. Female C57BL6/J mice were placed on either a high-fat diet (HFD; 35.8% fat and 20.2% protein by nutritional content) or an isocaloric control diet (CD; 13% fat and 25% protein) for six weeks. All mice were then maintained on CD for eight weeks. We found that whereas metabolic parameters (weight, glucose tolerance, and cholesterol levels) of the HFD mice returned to normal after this “diet reversal” period, several oocyte defects were not reversible. Oocytes from the diet reversal mice demonstrated a significantly higher percentage of abnormal meiotic spindles than those from control mice. The HFD diet reversal GV oocytes also had lower mitochondrial membrane potential, lower levels of ATP and citrate, and higher percentages of abnormal lipid accumulation and distribution and abnormally distributed mitochondria than oocytes from control mice. Thus, despite normalization of weight, glucose utilization, and cholesterol levels eight weeks after switching from a high fat to a regular chow, oocytes from diet reversal mice exhibited significantly higher rates of meiotic spindle, lipid, and mitochondrial defects than found in mice maintained on regular chow. These results suggest that the negative effects of an obesogenic diet on oocyte quality are not as reversible as the overall metabolic parameters. These data may provide better insight when counseling obese women regarding reproductive options and success.

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Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle

Cited by 40 publications

References 66 publications

Revascularization and muscle adaptation to limb demand ischemia in diet-induced obese mice

Revascularization and muscle adaptation to limb demand ischemia in diet-induced obese mice

Modulating effects of exercise training regimen on skeletal muscle properties in female polo ponies

Adverse effects of obesity and/or high-fat diet on oocyte quality and metabolism are not reversible with resumption of regular diet in mice

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