Effects of Herbicides on RNA and Protein Syntheses

Moreland, Donald E.; Malhotra, S. S.; Gruenhagen, R. D.; Shokraii, E. Hosseini

doi:10.1017/s0043174500054801

Cited by 83 publications

(56 citation statements)

References 50 publications

(48 reference statements)

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“…Results reported herein for both intact and excised root tips indicate that the initial effects of trifluralin are not on protein biosynthesis . This is in agreement with reports of studies with excised soybean hypocotyls [5] and corn mesocotyl and soybean hypocotyl sections [14] . However, if the synthesis of a specific protein was affected, the techniques used in this study would not detect changes at that level .…”

Section: 2 14 C-leucine Uptake and Incorporationsupporting

confidence: 93%

“…Moreland et al . [14] found no measurable effect on ATP generation, orotate incorporation into RNA or leucine incorporation into protein in maize mesocotyl or soybean (Glycine max L.) hypocotyl sections . Protein synthesis (14C-leucine incorporation) in tobacco callus was inhibited, but 5 to 7 days after treatment [27] .…”

Section: Introductionmentioning

confidence: 77%

“…Intact and excised root tips were incubated with 14 protein hydrolysate was the same from 0 to 8 min and at 20 min ( Figure 2) . When the precursor pool is saturated (calcualted to be 2 .8 and 3 .3 min for control and treated tissue, respectively), the incorporation of 14C-leucine into protein would theoretically become linear ; accumulation of radioactiv- ity into protein of untreated tissue exhibited linearity after about 5 min ( Figure 2).…”

Section: 2 14 C-leucine Uptake and Incorporationmentioning

confidence: 99%

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Protein synthesis in trifluralin-treated corn root tips

Camper

Ellers

1989

Plant Growth Regul

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The effect of trifluralin (a,a,a-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) on protein synthesis in corn (Zea mays) root tips was determined . 14C-leucine uptake and subsequent incorporation into protein in intact and excised root tips was measured . Root tips were treated with and without 15 pM trifluralin for 2 hr ; incorporation of 14 C-leucine was observed during a 20-min interval . Total amino acid content in the soluble pool and protein hydrolysate was reduced in the excised tissue by the herbicide . Kinetic analysis showed trifluralin had no effect on endogenous leucine pool size nor on the rate of protein synthesis in intact tissue . Uptake was unaffected; however, in excised tissue uptake increased 100% over the control . While 14C-leucine content was greater in both the soluble pool and protein in treated, excised root tips, analysis showed the apparent increase in protein synthesis was in response to increased pool size .

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Section: 2 14 C-leucine Uptake and Incorporationsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 77%

Section: 2 14 C-leucine Uptake and Incorporationmentioning

confidence: 99%

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Protein synthesis in trifluralin-treated corn root tips

Camper

Ellers

1989

Plant Growth Regul

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“…A single one-halfcentimeter shoot segment immediately above the seed and a single 1.0-cm root tip segment were excised from the seedlings and kept at 4~ until a sufficient amount had been obtained. The incubation medium used for this study contained 0.01 M KHzPO 4 buffer (pH 6.0) containing 1% sucrose (w/v) and 40 IxM chloramphenicol (Moreland et al 1969).…”

Section: Excised Tissuesmentioning

confidence: 99%

Butachlor influence on selected metabolic processes of plant cells and tissues

Chang

Ashton

Bayer

1985

J Plant Growth Regul

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Time-and concentration-course studies were conducted to determine the effects of butachlor (N-[butoxymethyl]-2-chloro-2',6'-diethylacetanilide) on photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis using isolated leaf cells of red kidney bean (Phageolus vulgaris L.). At the 2-h incubation period, butachlor inhibited photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis 99, 99, 96, and 81% respectively at 100 ~xM, and 0, 19, 17, and 40% respectively at 10 p.M. At 100 jxM and 15-, 30-, and 60-min incubations, RNA synthesis was inhibited 20, 76 and 90% respectively, and lipid synthesis 35, 48, and 62% respectively; ph6tosynthesis and protein synthesis were inhibited over 90% at all of these time periods. The effects of 50 txM butachlor on protein and RNA synthesis in rice (Oryza sativa L.) and barnyardgrass (Echinochloa crusgalli L.) root and shoot segments were also investigated. Protein synthesis was inhibited in both species and to a greater degree in roots (81-90%) than in shoots (55-65%). RNA synthesis was inhibited 33% in barnyardgrass roots but not significantly in barnyardgrass shoots or either organ of rice.

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“…Streptomycin, at the concentration used in these experiments, has been reported to control bacterial growth without seriously affecting precursor incorporation into RNA and protein of higher plant tissues (8). Incorporation of a labeled nucleotide such as 14C-ATP into RNA and of a labeled amino acid such as 14C-leucine into a polypeptide chain has been widely used to measure and study de novo RNA and protein synthesis in a variety of plant tissues (8,14).…”

Section: Incubationmentioning

confidence: 99%

Picloram effects on ribonucleic acid and protein biosynthesis in excised plant tissues

Sharma¹,

Born²

1972

Can. J. Bot.

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SHARMA, M. P., and W. H. VANDEN BORN. 1972. Piclorarn effects on ribonucleic acid and protein biosynthesis in excised plant tissues. Can. J. Bot. 50: 2489-2501. Picloram (4-amino-3,5,6-trichloropicolinic acid), both at growth-promoting (10 pg/ml) and growthinhibiting (500 pg/rnl) concentrations decreased the loss of RNA (ribonucleic acid) and protein of excised soybean (Glycitze max (L.) Merr. cv. Harosoy 63) hypocotyl, barley (Hordeum vulgare L. cv. Parkland) coleoptile, barley leaf, and Canada thistle (Cirsiurn arvense (L.) Scop.) leaf tissues during an 8-h incubation. At a growth-promoting concentration picloram enhanced RNA and protein biosynthesis (incorporation of 14C-labeled precursors), whereas at a growth-inhibiting concentration it inhibited such synthesis. Incorporation of radioactivity into RNA and protein of particulate and supernatant fractions (separated at 20 000 X g) appeared to be equally sensitive to piclorarn. Actinomycin D and cyclohexirnide in the incubation medium markedly inhibited both normal and picloram-induced growth and RNA and protein biosynthesis. Purornycin was less effective in inhibiting growth and protein biosynthesis. Picloram, gibberellic acid, and indoleacetic acid all promoted growth and RNA and protein biosynthesis though they did so at different concentrations and to varying degrees. Our results indicate that picloram has a role in altering both net synthesis of RNA and protein and some processes in the degradative metabolism of these constituents. SHARMA, M. P., et W. H. VANDEN BORN. 1972. Picloram effects on ribonucleic acid and protein biosynthesis in excised plant tissues. Can. J. Bot. SO: 2489-2501. Le picloram (acide 4-amino-3,5,6-trichloropicolinique), a des concentrations stirnulatrices (10 pg/rnl) aussi bien qu'inhibitrices (500 pg/ml) de la croissance, a dirninue la perte de RNA (acide ribonucleique) et de proteines dans l'hypocotyle isole du soya (Glycine max (L.) Merr. cv. Harosoy 63), le coleoptile et la feuille de I'orge (Hordeum vulgare L. cv. Parkland) et les tissus foliaires du chardon (Cirsilrm arvense (L.) Scop.) au cours d'une incubation de 8 h. A une concentration qui stirnule la croissance, le picloram a stirnu16 la biosynthkse du RNA et des proteines (incorporation de precurseurs marques au 14C), alors qu'i une concentration qui inhibe la croissance ces bi,osynthkses ont 6tB inhibees. Les incorporations de radio-activite dans le RNA et les proteines a partir des fractions granuleuse et surnageante (separkes a 20 000 X g) ont sernbl6 Cgalement sensibles au piclorarn. L'actinornycine D et la cycloheximide ajoutks au milieu d'incubation ont forternent inhibe la croissance et la biosynthtse norrnale du RNA et des protdines aussi bien que de celles induites par le piclorarn. La purornycine s'est avtree rnoins efficace 1 inhiber la croissance et la biosynthkse des protCines. Le picloram, l'acide gibbkrellique et I'acide indole-acktique ont tous trois stirnulk la croissance et la biosynthkse du RNA et des proteines bien qu'elles le firent des concentrations...

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Effects of Herbicides on RNA and Protein Syntheses

Cited by 83 publications

References 50 publications

Protein synthesis in trifluralin-treated corn root tips

Protein synthesis in trifluralin-treated corn root tips

Butachlor influence on selected metabolic processes of plant cells and tissues

Picloram effects on ribonucleic acid and protein biosynthesis in excised plant tissues

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