Effects of Hepatitis C Virus Infection on Human Sperm Chromosomes

Zhu, Yiwen; Ma, Mingfu; Huang, Jihua; Hou, Zhuoni; Wang, Dian; Zhang, Danyan; Xie, Qi; Wan, Ling; Zhao, Lijing; Li, Wei; Li, Lianbing; Huang, Tian-Hua

doi:10.7754/clin.lab.2015.150705

Cited by 8 publications

(13 citation statements)

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“…It is worth mentioning that, besides HBV, there are more emerging infectious diseases virus, such as hepatitis C virus (HCV), human immunodeficiency virus (HIV), severe acute respiratory syndrome virus (SARS), Ebola virus and Zika virus (ZIKV), which pose a serious threat to human health [48][49][50][51][52]. Lately, some studies begin to investigate the true vertical transmission of HCV and HIV via germline [34,[53][54][55][56], but the research on such transmission of SARS, Ebola and ZIKA viruses is still a blank. To clarify whether the aforementioned viral genes are transmitted via germline and their expressional regulation in embryo would make a great contribution to exploring the interplay mechanism between the viruses and host cells and to maintaining human reproductive health.…”

Section: Discussionmentioning

confidence: 99%

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Zhong

Liu

Ahmed

et al. 2017

Reproductive Toxicology

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Hepatitis B virus (HBV) can invade the male germline, and sperm-introduced HBV genes could be transcribed in embryo. This study was to explore whether viral gene transcription is regulated by host genes. Embryos were produced by in vitro fertilization of hamster oocytes with human sperm containing the HBV genome. Total RNA extracted from test and control embryos were subjected to SMART-PCR, SSH, microarray hybridization, sequencing and BLAST analysis. Twenty-nine sequences showing significant identity to five human gene families were identified, with CSH2, EIF4G2, PCBD2, PSG4 and TTN selected to represent target genes. Using qRT-PCR, when CSH2 and PCBD2 (or EIF4G2, PSG4 and TTN) were silenced by RNAi, transcriptional levels of HBV s and x genes decreased (or increased). This is the first report that host genes participate in regulation of sperm-introduced HBV gene transcription in embryo, which is critical to prevent negative impact of HBV infection on early embryonic development.

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Section: Discussionmentioning

confidence: 99%

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Zhong

Liu

Ahmed

et al. 2017

Reproductive Toxicology

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“…Частота хромосомных аберраций была ста тистически значимо выше в инфицированных сперматозоидах по сравнению с контрольной группой (10,85 % против 5,96 %). Авторы пришли к выводу, что вертикальная передача ВГС через мужские половые клетки вероятна, и она может быть причиной наследственных генетических аномалий, связанных с хромосомными аберрациями [48]. В дискордантных парах, в которых мужчина инфицирован ВГС и которые прибегают к помощи ВРТ, рекомендуется осуществлять промывание сперматозоидов, которое снижает вирусную нагрузку, хотя и не устраняет риск полностью.…”

Section: вирус гепатита сunclassified

“…В дискордантных парах, в которых мужчина инфицирован ВГС и которые прибегают к помощи ВРТ, рекомендуется осуществлять промывание сперматозоидов, которое снижает вирусную нагрузку, хотя и не устраняет риск полностью. Кроме того, поскольку экспериментальные исследования, проведенные на животных, показали, что противовирусное лечение оказывает тератогенный и эмбриоцидный эффект, беременность рекомендуют отложить как минимум на 6 мес после завершения терапии [49].…”

Section: вирус гепатита сunclassified

Viral infection of sperm. Part 2. Human herpes viruses, human immunodeficiency virus, hepatitis C virus, Zika virus (review)

Брагина

2021

Androl. genit. hir.

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Intragametal viral infection of spermatozoa can cause true vertical transmission of viruses through germ cells. Currently, human immunodeficiency virus, hepatitis C viruses, herpes simplex virus, cytomegalovirus, Zika virus have been detected in spermatozoa. The possibility of vertical transmission of human immunodeficiency virus, cytomegalovirus, herpes simplex virus and Zika virus has been proven.Intragametal infection of spermatozoa with viruses of the herpes group leads to abnormalities in the development of the embryo and can cause spontaneous abortions both during natural conception and when using assisted reproductive technologies.The development of adequate methods for diagnosing an intragametal spermatozoa virus infection will make it possible to find out, at least in some patients, the cause of infertility and pregnancy abnormalities and apply appropriate antiviral therapy in preparation for natural conception or the use of assisted reproductive technologies.

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“…И хотя в эксперименте показан му-тагенный эффект HCV на хромосомы сперматозоидов с риском генетиче-ских изменений и/или хромосомных аберраций вследствие способности гена С HCV проникать через мембрану сперматозоида и интегрироваться в геном, возможность инфицирования ребенка от больного гепатитом С отца при здоровой матери показана не была [19,20]. В случае если мужчина по каким-либо причинам отказывается от лечения гепатита С до проведения ЭКО, следует прибегнуть к отмыванию спермы или ее интрацитоплазматиче-ской инъекции [21].…”

Section: рисунок 4 профилактика внутриутробного инфицирования Hbv теunclassified

Viral hepatitis in women of reproductive age

Зайцев

2017

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A considerable number of various liver diseases that are associated with the course of pregnancy are quite common and occur at least three percent of pregnant women.This pathology plays an important role and is one of the most important reasons for the development of a wide range of different pathologies in pregnant women, in fetuses and newborns. The problem of changing functional liver tests during pregnancy acquires special sharpness and significance in connection with the not-so-recent role of liver pathology in mortality in maternal and perinatal mortality.In recent years, based on a large number of observations and statistical data, important information has been obtained on the details of the mechanism of this pathology, developed and refined diagnostic methods, as well as detailed schemes for treating liver diseases in pregnant women at different stages of pregnancy. Collected and scrupulously processed extensive data on the change in a variety of functional liver tests at various stages of pregnancy, both under normal and with complicated flow.Particular attention is paid to such pathology as preeclampsia, eclampsia, acute fatty liver disease of pregnant women, HELLP syndrome, intrahepatic cholestasis of pregnant women, uncontrollable vomiting of pregnant women, liver rupture, in the diagnosis of which one of the leading, decisive roles is assigned to the change in functional liver tests.In our review, the main emphasis was made in the direction of changing various significant indicators of functional hepatic tests in pregnant women both in physiologically developing pregnancy and in various liver diseases, both as a consequence of pregnancy and not directly related to it. The article also highlights the main mechanisms of these changes. The publication provides the latest information on the timing of full or partial normalization of a wide range of functional liver tests both after delivery and in the natural course of the disease.

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Effects of Hepatitis C Virus Infection on Human Sperm Chromosomes

Cited by 8 publications

References 0 publications

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo

Viral infection of sperm. Part 2. Human herpes viruses, human immunodeficiency virus, hepatitis C virus, Zika virus (review)

Viral hepatitis in women of reproductive age

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