Effects of heparinoids from algae on hemostasis and their action on the cycle cell

Extraction process optimization of sulfated galactan-rich fractions from Hypnea musciformis in order to obtain antioxidant, anticoagulant, or immunomodulatory polysaccharides

“…Using an extraction methodology based on proteolytic digestion with a proteolytic enzyme and two volumes of NaCl for 18 h at 60°C, sulfated galactans were obtained from H. musciformis with antioxidant (Alves et al 2012a) and anticoagulant activities (Alves et al 2012b). It was possible to obtain sulfated polysaccharides with different activities and we were interested in determining the optimal method of extraction to obtain polysaccharides with particular biological activities, such as anticoagulant, antioxidant, and immunomodulatory activities.…”

“…All sulfated galactans synthesized by algae are anionic, and most galactans have a high molecular mass. These two features are mainly responsible for the different pharmacological properties of the galactans produced by seaweed (Pomin 2010), including their antiproliferative (Costa et al 2010), antioxidant (Costa et al 2010;Alves et al 2012a), antitumor (Lins et al 2009), gastroprotective (Damasceno et al 2013), anticoagulant (Farias et al 2000;Alves et al 2012b), antiviral (Carlucci et al 1997), and immunostimulatory (Zhou et al 2004) activity.…”

“…Previous studies by our group using an extraction methodology based on proteolytic digestion of seaweed and two volumes of aqueous solution of sodium chloride (NaCl) incubated at 60°C for 18 h yielded sulfated galactans from the red seaweed H. musciformis with antioxidant (Alves et al 2012a) and anticoagulant activities (Alves et al 2012b). Thus, sulfated polysaccharides with different biological activities were obtained with the same extraction methodology.…”