1995
DOI: 10.1111/j.1399-6576.1995.tb04150.x
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Effects of heparin on the inhibitory action of protamine on endothelium‐mediated vasorelaxation

Abstract: The precise mechanism(s) of inhibitory action of protamine on endothelium-mediated vasorelaxation has not been fully elucidated. In addition, no information is available regarding the effects of a heparin-protamine complex on the endothelium-mediated relaxation. Employing isometric tension recording methods, we studied the effects of heparin, an anionic substance, on the protamine-induced inhibition of acetylcholine (ACh)-induced vasorelaxation in isolated rabbit small mesenteric artery. Protamine (> or = 50 m… Show more

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Cited by 9 publications
(3 citation statements)
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References 35 publications
(34 reference statements)
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“…Protamine and other basic polyamino acids rich in L-arginine, lysine, or ornithine have been shown to produce endothelium-dependent vasodilation by inducing the generation of endothelium-derived relaxing factor (nitric oxide) (23,37). However, prolonged exposure to such compounds results in refractoriness to stimulants of endothelium-mediated vasorelaxation (1,23). To explain this paradoxical observation, Ignarro et al (23) have proposed that such basic polyamino acids serve as partial substrates for the enzyme system that catalyzes the production of endothelium-derived relaxing factor, a process that involves the generation of nitric oxide from L-arginine.…”
Section: Potential Non-heparin Effects Of Protaminementioning
confidence: 99%
“…Protamine and other basic polyamino acids rich in L-arginine, lysine, or ornithine have been shown to produce endothelium-dependent vasodilation by inducing the generation of endothelium-derived relaxing factor (nitric oxide) (23,37). However, prolonged exposure to such compounds results in refractoriness to stimulants of endothelium-mediated vasorelaxation (1,23). To explain this paradoxical observation, Ignarro et al (23) have proposed that such basic polyamino acids serve as partial substrates for the enzyme system that catalyzes the production of endothelium-derived relaxing factor, a process that involves the generation of nitric oxide from L-arginine.…”
Section: Potential Non-heparin Effects Of Protaminementioning
confidence: 99%
“…Protamine is a protein rich in the L-arginine amino acid, precursor of nitric acid [13] and in our study vascular relaxation is completely abolished when the arterial segment is denuded of endothelium. Therefore, this protamine-derived vasodilatation appears to be an intrinsic action of this drug when it interacts with vascular endothelium, although some authors believe that when the heparin-protamine complex is formed, this relaxing effect is annulled [11,14].…”
Section: Discussionmentioning
confidence: 89%
“…Mechanical responses were measured by attaching the strip to a strain gauge (UI-2 type, Shinko, Tokyo, Japan) in a chamber of 0.9ml capacity as previously described [9][10][11][12][13]. The strip was horizontally mounted in the chamber on a microscope stage, and then stretched to ~1.1-1.2 times the resting length to obtain the maximum contractile response to high K +.…”
Section: Tension Measurementmentioning
confidence: 99%