Effects of Heparin Coating of Cardiopulmonary Bypass Circuits on In Vitro Oxygen Free Radical Production During Coronary Bypass Surgery

Bozdayı, A. Mithat; Borowiec, Jan; Nilsson, Leif; Thelin, Stefan; Hansson, H

doi:10.1111/j.1525-1594.1996.tb04588.x

Cited by 17 publications

(7 citation statements)

References 22 publications

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“…Excellent resuscitation was obtained (data not shown), and no blood coagulation or microclots were found in the coated devices. Numerous studies [27][28][29] in which the same type of coating in cardiopulmonary bypass was used have shown greatly improved outcome. Release of heparin from the coating was virtually undetectable.…”

Section: Discussionmentioning

confidence: 99%

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

Liachenko

Tang

Hamilton

et al. 1998

Stroke

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Background and Purpose-Because noninvasive physiological monitoring of cerebral blood flow, metabolic integrity, and brain ion and water homeostasis can now be accomplished with new, state-of-the-art MR spectroscopy and imaging techniques, it is appropriate to develop controllable and reproducible animal models that permit prolonged circulatory arrest and resuscitation in the magnet and also allow for studies of long-term survival and outcome. We have developed such a model in rats that involves minimal surgical preparations and can achieve resuscitation remotely within precisely controlled time. Methods-Cardiac arrest was induced by asphyxiation, the duration of which ranged from 8 to 24 minutes. Resuscitation was achieved remotely by a slow, intra-aortic infusion of oxygenated blood (withdrawn either from the same rat before asphyxia or from a healthy donor rat) along with a resuscitation cocktail containing heparin (50 U/100 g), sodium bicarbonate (0.1 mEq/100 g), and epinephrine (4 g/100 g). The body temperature was measured by a tympanic thermocouple probe and was controlled either by a heating pad (constant tympanic temperatureϭ37°C) or by warm ambient air (constant air temperatureϭ37°C). Interleaved 31 P/ 1 H nuclear magnetic resonance (NMR) spectroscopy was used in a selected group of rats to measure the cerebral metabolism before and during approximately 20 minutes of circulatory arrest and after resuscitation. Results-The overall success rate of resuscitation, irrespective of the duration of cardiac arrest, was 82% (51 of 62). With a programmed infusion pump, the success rate was even higher (95%). The survival time for rats subjected to 15 and 19 minutes of asphyxia with core temperature tightly controlled was significantly lower than that with ambient temperature control (PϽ0.001 and PϽ0.04, respectively). High-quality NMR spectra can be obtained continuously without interference from the resuscitation effort. Final histological examinations taken 5 days after resuscitation showed typical neuronal damages, similar to those found in other global ischemia models. Conclusions-Because the no-flow time and resuscitation time can be precisely controlled, this outcome model is ideally suited for studies of ischemic and reperfusion injuries in the brain and possibly in other critical organs, permitting continuous assessment of long-term recovery and follow-up in the same animals. (Stroke. 1998;29:1229-1239.)

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Section: Discussionmentioning

confidence: 99%

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

Liachenko

Tang

Hamilton

et al. 1998

Stroke

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“…The benefits gained by heparin-coated circuits are a restriction of complement activation (mainly of factor C5a) ranging between 25% and 45% [61,62], and a decrease in the inflammatory response via the limitation of complement activation and binding of phospholipase A2 [63]. The final result is improved clinical outcomes, thanks to a decrease in the inflammatory response being considerable by reduced levels of IL-6, IL-8, E-selectin, lactoferin, myeloperoxidase, integrin, selectin, platelet b-thromboglobulin, and oxygen-free radicals [19,[63][64][65][66]. Nevertheless, heparin appears not to influence thrombin production and fibrinolysis [67].…”

Section: Post-bypass Inflammatory Responsementioning

confidence: 99%

The “benefits” of the mini-extracorporeal circulation in the minimal invasive cardiac surgery era

Baikoussis

Papakonstantinou

Apostolakis

2014

Journal of Cardiology

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Mini-extracorporeal circulation (MECC) constitutes a novel miniaturized cardiopulmonary bypass (CPB) circuit, heparin-coated and primed with aprotinin. Its membrane oxygenation is similar to conventional cardio-pulmonary bypass (CCPB), but it is a completely closed-volume system due to the lack of the venous reservoir which has been removed. In a mini circuit, the reservoir is the patient himself. Consequently, air entering the venous cannula is avoided. Nevertheless, the capabilities of MECC have been expanded either by the inclusion of a suction device that is only activated on direct contact with liquid in some circuits or by postoperative autotransfusion of the wrecked erythrocytes by a separate suction device with a cell-saver. Although the tubing diameter is similar between the two systems, the tubing length of the MECC is around half that of the CCPB, resulting in the restriction of priming volume. As a consequence, a higher hematocrit thus a limited need for perioperative blood transfusion is achieved due to less hemodilution. In addition, the inflammatory response is also diminished as a result of less artificial surface area interacting with blood. Finally, a lower dose of heparin is required prior to MECC than prior to CCPB.

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“…The bactericidal effect and induction of inflammation by reactive oxygen produced by granulocytes are known as the immune function, there are some reports about pathophysiology of each organ (lung [70][71][72][73][74][75], cardiovascular [76][77][78], kidney [79,80], liver [81], etc.) and evaluation of treatment (drug [79][80][81][82][83][84][85][86][87][88][89][90][91][92][93], food factor [94], operation [95], etc.) by radical oxygen measurement of neutrophilic granulocyte from human blood samples.…”

Section: Possibility Of Clinical Application Of Reactive Oxygen Measumentioning

confidence: 99%

Oxidative Stress and Diseases: Current Topics and Perspective with Clinical Application in Japan

Ichiishi¹,

Ohtake²,

Satoh³

et al. 2016

J Aging Sci

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Evaluation of oxidative stress and diseases have markedly progressed and developed in basic science, and various trials of prevention and treatment have recently been performed at clinical levels, making remarkable progress in medical practical field. This report will introduce recent topics in Japan and other countries and review Japanese reports of oxidative stress pathways and development of healthy products, current topics and problems with molecular hydrogen, oxidative stress markers attracting attention at clinical practices, recent knowledge on oxidative stress and the immune system, and new development in regenerative medical treatment. A problem with the above development in clinical fields and application of oxidative stress is that health foods and beverages allegedly effective for reducing oxidative stress are already on the market but there are more than a few of them have a poor scientific evidence and their effects are questionable. Unquestionable bases and evidence of 'oxidative stress and diseases' have already accumulated in basic medicine. I hope these will be actively applied in clinical fields, accumulate evidence by performing systematic clinical studies and trials, and actually facilitate health promotion, disease prevention, and treatment effects in humans, making great contributions at actual medical practical care.

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Effects of Heparin Coating of Cardiopulmonary Bypass Circuits on In Vitro Oxygen Free Radical Production During Coronary Bypass Surgery

Cited by 17 publications

References 22 publications

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

The “benefits” of the mini-extracorporeal circulation in the minimal invasive cardiac surgery era

Oxidative Stress and Diseases: Current Topics and Perspective with Clinical Application in Japan

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