Effects of Helicobacter pylori and bile on N-methyl-N′-nitro-N′-nitrosoguanidine exposed antral mucosa of C57BL/6 mice

Abstract

The aim of this study was to evaluate the early influence of Helicobacter pylori infection on cell kinetics in the antral mucosa of mice exposed to N-methyl-N'-nitro-N'-nitrosoguanidine (MNNG) and bile alone or in combinations. Four hundred and one C57BL/6 male and female mice were assigned into seven treatment groups and one non-treated control group. The gastric antrums were assessed by histology and immunohistochemistry for studies of cell proliferation and apoptosis at 32 and 44 weeks. One female and one male mouse had developed dysplastic adenomas in the pylorus mucosa and one male animal had dysplastic proliferation in the antrum. Only one of these lesions occurred in a H. pylori colonized animal. H. pylori infection significantly increased the cell proliferation at 32 weeks and promoted the cell proliferation in the MNNG and bile group at 44 weeks. Female mice showed less increase in cell proliferation than did the males. No change in apoptosis was seen in any of the groups. Bile had no promotional effect on cell proliferation. These results indicate that H. pylori infection has the potential to alter epithelial cell kinetics as well as antrum mucosa of an animal species that is regarded as resistant to MNNG. However, this change is not sufficient to promote the early development of neoplastic lesions.

How useful are the rodent animal models of gastric adenocarcinoma?

“…In contrast with rats and gerbils, mice are relatively resistant to the induction of gastric carcinomas by MNNG (reviewed in Fox, 6 Loogna et al 33 . and Danon and Eaton 34 ).…”

Synthetic CDCA Derivatives-Induced Apoptosis of Stomach Cancer Cell Line SNU-1 Cells

“…Thus, studies of the effects of bile acid on stomach cancer are lacking. In an experiment evaluating the early influence of H. pylori infection on cell kinetics in the antral mucosa of mice, bile had no promotional effect on cell proliferation (5). However, in another experiment, the combination with taurocholic acid showed a synergistic effect on H. pylorienhanced epithelial cell proliferation (6).…”

Characterization of a Helicobacter hepaticus putA Mutant Strain in Host Colonization and Oxidative Stress

“…hepaticus is closely related to the gastric human pathogen Helicobacter pylori, which is responsible for acute gastric inflammation that can then progress from superficial gastritis to peptic ulceration and gastric cancer. H. pylori is classified by the World Health Organization as a group 1 carcinogen (8,(31)(32)(33). One of the important features in the pathogenesis of Helicobacter infection is its ability to establish persistent colonization within the gastrointestinal mucosa.…”