Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles

Mota, Rita; Pereira, Sara B.; Meazzini, Marianna; Fernandes, Rui; Santos, Álvaro da Silva; Evans, Caroline; Philippis, Roberto De; Wright, Phillip C.; Tamagnini, Paula

doi:10.1016/j.jprot.2015.03.004

Cited by 80 publications

(39 citation statements)

References 69 publications

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“…As the first tool of microorganisms to adapt to adverse environments, extracellular polymeric substances (EPS) can prevent heavy metal ions from entering the cells of the microorganism [16]. Considering their minimal nutritional requirements, easy cultivation and rapid metal removal kinetics, EPS-producing cyanobacteria can be an advantageous choice than other microorganisms for treatment of metal wastewater [17]. Cyanobacteria, or blue-green algae, are common microorganisms with the capacity of producing a great deal of EPS [18].…”

Section: Introductionmentioning

confidence: 99%

Behavior and Mechanism of Cesium Biosorption from Aqueous Solution by Living Synechococcus PCC7002

Chai

et al. 2020

Microorganisms

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Many efforts have focused on the adsorption of metals from contaminated water by microbes. Synechococcus PCC7002, a major marine cyanobacteria, is widely applied to remove metals from the ocean’s photic zone. However, its ability to adsorb cesium (Cs) nuclides has received little attention. In this study, the biosorption behavior of Cs(I) from ultrapure distilled water by living Synechococcus PCC7002 was investigated based on kinetic and isotherm studies, and the biosorption mechanism was characterized by Fourier-transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectrometry, and three-dimensional excitation emission matrix fluorescence spectroscopy. Synechococcus PCC7002 showed extremely high tolerance to Cs ions and its minimal inhibitory concentration was 8.6 g/L. Extracellular polymeric substances (EPS) in Synechococcus PCC7002 played a vital role in this tolerance. The biosorption of Cs by Synechococcus PCC7002 conformed to a Freundlich-type isotherm model and pseudo-second-order kinetics. The binding of Cs(I) was primarily attributed to the extracellular proteins in EPS, with the amino, hydroxyl, and phosphate groups on the cell walls contributing to Cs adsorption. The biosorption of Cs involved two mechanisms: Passive adsorption on the cell surface at low Cs concentrations and active intracellular adsorption at high Cs concentrations. The results demonstrate that the behavior and mechanism of Cs adsorption by Synechococcus PCC7002 differ based on the Cs ions concentration.

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Section: Introductionmentioning

confidence: 99%

Behavior and Mechanism of Cesium Biosorption from Aqueous Solution by Living Synechococcus PCC7002

Chai

et al. 2020

Microorganisms

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“…However, it was recently reported that in Cyanothece sp. CCY 0110, the presence of heavy metals significantly affected its protein profile, in particular with regard to the proteins associated with photosynthesis, CO 2 fixation and carbohydrate metabolism, but did not enhance the amount of RPS released by the cells [ 33 ]. Between other possible elicitors, substances, such as glyoxylate, valerate, acetate or EDTA, are also reported [ 3 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Role of Cyanobacterial Exopolysaccharides in Phototrophic Biofilms and in Complex Microbial Mats

Rossi

Philippis

2015

Life

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303

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Exopolysaccharides (EPSs) are an important class of biopolymers with great ecological importance. In natural environments, they are a common feature of microbial biofilms, where they play key protective and structural roles. As the primary colonizers of constrained environments, such as desert soils and lithic and exposed substrates, cyanobacteria are the first contributors to the synthesis of the EPSs constituting the extracellular polymeric matrix that favors the formation of microbial associations with varying levels of complexity called biofilms. Cyanobacterial colonization represents the first step for the formation of biofilms with different levels of complexity. In all of the possible systems in which cyanobacteria are involved, the synthesis of EPSs contributes a structurally-stable and hydrated microenvironment, as well as chemical/physical protection against biotic and abiotic stress factors. Notwithstanding the important roles of cyanobacterial EPSs, many aspects related to their roles and the relative elicited biotic and abiotic factors have still to be clarified. The aim of this survey is to outline the state-of-the-art of the importance of the cyanobacterial EPS excretion, both for the producing cells and for the microbial associations in which cyanobacteria are a key component.

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“…Copper is an essential trace metal for microalgae too (Raven et al 1999;Balamurugan and Schaffner 2006;Peers and Price 2006). However, in excess, all metals are deleterious to the cells (Mota et al 2015). Zinc is phytotoxic at 100 μg/g (Mazumdar and Das 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

Cao

Xie

Deng

et al. 2015

Environ Sci Pollut Res

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Effects of various concentrations of Cu(2+) and Zn(2+) (0.0, 0.1, 0.25, 0.5, or 1.0 mg/L) on the growth, malondialdehyde (MDA), the intracellular calcium, and physiological characteristics of green algae, Cladophora, were investigated. Low Zn(2+) concentrations accelerated the growth of Cladophora, whereas Zn(2+) concentration increases to 0.25 mg/L inhibited its growth. Cu(2+) greatly influences Cladophora growth. The photosynthesis of Cladophora decreased under Zn(2+) and Cu(2+) stress. Cu(2+) and Zn(2+) treatment affected the content of total soluble sugar in Cladophora and has small increases in its protein content. Zn(2+) induced the intracellular calcium release, and copper induced the intracellular calcium increases in Cladophora. Exposure to Cu(2+) and Zn(2+) induces MDA in Cladophora. The stress concent of Cu(2+) was strictly correlated with the total soluble sugar content, Chla+Chlb, and MDA in Cladophora, and the stress concent of Zn(2+) was strictly correlated with the relative growth rate (RGR) and MDA of Cladophora.

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Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles

Cited by 80 publications

References 69 publications

Behavior and Mechanism of Cesium Biosorption from Aqueous Solution by Living Synechococcus PCC7002

Behavior and Mechanism of Cesium Biosorption from Aqueous Solution by Living Synechococcus PCC7002

Role of Cyanobacterial Exopolysaccharides in Phototrophic Biofilms and in Complex Microbial Mats

Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

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