Effects of heat stress on antioxidant defense system, inflammatory injury, and heat shock proteins of Muscovy and Pekin ducks: evidence for differential thermal sensitivities

Abstract

Rising temperatures are severely affecting the mortality, laying performance, and meat quality of duck. Our aim was to investigate the effect of acute heat stress on the expression of heat shock proteins (HSPs: HSP90, 70, 60, 40, and 10) and inflammatory factors (nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)) and antioxidant enzyme activity (superoxide dismutase (SOD), malondialdehybe (MDA), catalase (CAT), total antioxidant capacity (T-AOC)) in livers of ducks and to compare the thermal tolerance of Pekin and Muscovy ducks exposed to acute heat stress. Ducks were exposed to heat at 39 ± 0.5 °C for 1 h and then returned to 20 °C for 1 h followed by a 3-h recovery period. The liver and other tissues were collected from each individual for analysis. The mRNA levels of HSPs (70, 60, and 40) increased in both species, except for HSP10, which was upregulated in Muscovy ducks and had no difference in Pekin ducks after heat stress. Simultaneously, the mRNA level of HSP90 decreased in the stress group in both species. Morphological analysis indicated that heat stress induced tissue injury in both species, and the liver of Pekin ducks was severely damaged. The activities of several antioxidant enzymes increased in Muscovy duck liver, but decreased in Pekin duck. The mRNA levels of inflammatory factors were increased after heat stress in both duck species. These results suggested that heat stress could influence HSPs, inflammatory factors expression, and the activities of antioxidant enzymes. Moreover, the differential response to heat stress indicated that the Muscovy duck has a better thermal tolerance than does the Pekin duck.

Effect of dietary nutmeg oil on heat‐stress tolerance‐related parameters in Korean native chicken reared under hot temperature

“…Heat stress is known to promote tissue injury and severe liver damage in ducks (Zeng et al 2014). In addition, elevated serum levels of AST and ALT are indicative of the destruction of hepatocytes and liver dysfunction (Jaensch, Cullen, & Raidal, 2000;Tessari et al, 2010).…”

Effects of Fennel Seed Powder Supplementation on Growth Performance, Carcass Characteristics, Meat Quality, and Economic Efficiency of Broilers under Thermoneutral and Chronic Heat Stress Conditions

“…The first group was placed in an independent temperature-controlled room at 23 ± 2 • C. The broiler chicks from the second group were placed in a heat-stressed room and exposed to chronic heat stress conditions (32 ± 2 • C) for seven hours per day from 8:00 a.m. to 3:00 p.m. The indoor relative humidity and temperature under thermoneutral conditions during the experimental period for the broiler was 51.2% and 23.1 • C, 57% and 23.9 • C, and 53% and 24.7 • C during days [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], and 34-41 of age, respectively. Under chronic heat stress conditions, the indoor relative humidity and temperature was 44.9% and 31.5 • C, 47.8% and 31.8 • C, and 41.8% and 31.9 • C during days [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], and 34-41 of age, respectively.…”

Effects of Selenium Deficiency in the Environment on Antioxidant Systems of Wumen Semi-Fine Wool Sheep

“…It can reflect the compensatory capacity to external stimuli and the metabolism capacity of free radicals in organisms. The decreased function of the T-AOC defense system can cause other diseases [41]. The expression level of NOS would increase and a large amount of NO would be released when the animal is in selenium deficiency for a long time, and the change of NO metabolism would cause organ damage [42].…”

“…The reaction product of SOD is the substrate of GSH-Px [38]. The radical scavenging of O 2-· requires continuous catalysis from SOD, GSH-Px, CAT and LPO [41,42]. Therefore, SOD, GSH-Px and CAT plays a more important role in blocking membrane lipid peroxidation induced by free radicals.…”