Effects of heat stress on virus transmission and virus‐mediated apoptosis in whitefly Bemisia tabaci

Abstract

Tomato yellow leaf curl virus (TYLCV), a plant DNA virus of the genus Begomovirus, is transmitted by whiteflies of the Bemisia tabaci species complex in a persistent manner. Our previous study indicated that activation of the apoptosis pathway in whiteflies could facilitate TYLCV accumulation and transmission. Considering that temperature change can influence the spread of insect‐borne plant viruses, we focused on plant virus induced‐apoptosis to investigate the underlying mechanism of temperature regulation on plant virus transmission via an insect vector. We found that heat stress (40°C) on whiteflies could facilitate TYLCV accumulation and increase transmission to tomato plants. Despite upregulation of caspase‐1 and caspase‐3 gene expression, heat stress failed to induce an increase in the activation of cleaved caspase‐3 and DNA fragmentation in TYLCV‐infected whiteflies. However, our data failed to determine the role of heat stress in apoptosis modulation of insect–plant virus interplay while still providing clues to understand insect vectors and their transmitted plant viruses.