Effects of heat shock protein 70 knockdown on the tolerance of the brine shrimp Artemia franciscana to aquaculture-related stressors: Implications for aquatic animal health and production

Liqing,; Iryani, Mat Taib Mimi; Lv, Aijun; Sun, Jinfeng; Anirudhan, Anupa; Tan, Min Pau; Danish-Daniel, Muhd; Wong, Li Lian; Baruah, Kartik; Sorgeloos, Patrick; Muhammad, Tengku Sifzizul Tengku; Wang, Min; Liang, Yantao; Mok, Wen Jye; Sung, Yeong Yik

doi:10.1016/j.aquaculture.2021.737872

Cited by 8 publications

(3 citation statements)

References 53 publications

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“…californicus may explain how their mitochondria are able to avoid redox imbalance while operating when oxygen is unavailable [ 72 ]. Heat-shock proteins have been implicated in tolerating a number of processes beyond thermal and hypoxic stress including environmental pollutants [ 73 ], desiccation [ 74 ], osmolarity [ 75 ], as well as ammonia, salinity, and pH [ 76 ] to name a few. In contrast, exposure to slightly acidic conditions for 24 hours resulted in a different transcriptomic response—the upregulation of oxidative stress proteins—in Tigriopus japonicus , a sister species of T .…”

Section: Discussionmentioning

confidence: 99%

Hypoxia tolerance, but not low pH tolerance, is associated with a latitudinal cline across populations of Tigriopus californicus

Deconinck

Willett²

2022

PLoS ONE

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Intertidal organisms must tolerate daily fluctuations in environmental parameters, and repeated exposure to co-occurring conditions may result in tolerance to multiple stressors correlating. The intertidal copepod Tigriopus californicus experiences diurnal variation in dissolved oxygen levels and pH as the opposing processes of photosynthesis and cellular respiration lead to coordinated highs during the day and lows at night. While environmental parameters with overlapping spatial gradients frequently result in correlated traits, less attention has been given to exploring temporally correlated stressors. We investigated whether hypoxia tolerance correlates with low pH tolerance by separately testing the hypoxia and low pH stress tolerance separately of 6 genetically differentiated populations of T. californicus. We independently checked for similarities in tolerance for each of the two stressors by latitude, sex, size, and time since collection as predictors. We found that although hypoxia tolerance correlated with latitude, low pH tolerance did not, and no predictor was significant for both stressors. We concluded that temporally coordinated exposure to low pH and low oxygen did not result in populations developing equivalent tolerance for both. Although climate change alters several environmental variables simultaneously, organisms’ abilities to tolerate these changes may not be similarly coupled.

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Section: Discussionmentioning

confidence: 99%

Hypoxia tolerance, but not low pH tolerance, is associated with a latitudinal cline across populations of Tigriopus californicus

Deconinck

Willett²

2022

PLoS ONE

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“…Hsp70 was one of the most well-studied Hsps, which played an essential biological role in improving cell tolerance to stress and maintaining cell self-stability. 20 We cloned an Hsp70 from P. Clarkii (PcHsp70) in this study. The PcHsp70 protein contained a Dnak characteristic motif, an ATPbinding site motif, a non-organellar motif, and two signature sequences of the Hsp70 family, indicating that the PcHsp70 had ATPase activity and belonged to cytoplasmic Hsp70 of Dnak type.…”

Section: Discussionmentioning

confidence: 99%

Expression of Hsp70 in the red swamp crayfish, Procambarus clarkii in response to immune challenge

Peng

Chen

Han

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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A heat shock protein 70 (Hsp70) was identified from Procambarus clarkii (named as PcHsp70). The open reading frame of the PcHsp70 cDNA was 1917 bp, encoding a 638 aa protein with signature sequences of the Hsp70 family. Tissue distribution results showed that PcHsp70 has relatively high expression in immune-related tissues such as hepatopancreas, gill, and hemocytes. The expressions of PcHsp70 in hepatopancreas and gill were significantly induced by Gram-positive bacterium, Gram-negative bacterium, and white spot syndrome virus stimulation, indicating that PcHsp70 may be involved in the immune response to pathogen invasion. The research will provide a foundation for understanding the resistance and stress mechanism of Hsp70 in P. clarkii.

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“…The co-DEG-DEPs with consistent expression patterns at the transcriptional and translational levels were further investigated, which may play a key role in response to heat stress (Figure 7). HSP70 is a member of the heat shock protein family, which can prevent protein folding, refold denatured protein, and degrade denatured protein [43]. Moreover, it has been demonstrated that HSP70 plays an important role in combating heat stress, and the highly expressed HSP70 gene can improve the heat tolerance of cells, thus inhibiting the generation of oxygen radical and the apoptosis rate induced by heat stress [6,44,45].…”

Section: Co-expression Of Hsp70 and Gst At Gene And Protein Levels Un...mentioning

confidence: 99%

Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress

Shen,

Feng,

Zhao

et al. 2023

Antioxidants

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Heat stress is an increasingly concerning topic under global warming. Heat stress can induce organisms to produce excess reactive oxygen species, which will lead to cell damage and destroy the antioxidant defense of aquatic animals. Chinese mitten crab, Eriocheir sinensis, is sensitive to the change in water temperature, and parent crabs are more vulnerable during the breeding stage. In the present study, the multi-omics responses of parent E. sinensis gills to heat stress (24 h) were determined via transcriptome and proteome. The integrative analysis revealed that heat shock protein 70 (HSP70) and glutathione s-transferase (GST) were significantly up-regulated at gene and protein levels after heat stress, indicating that HSP70 and the antioxidant system participated in the regulatory mechanism of heat stress to resist oxidative damage. Moreover, the “Relaxin signaling pathway” was also activated at gene and protein levels under 30 °C stress, which implied that relaxin may be essential and responsible for reducing the oxidative damage of gills caused by extreme heat stress. These findings provided an understanding of the regulation mechanism in E. sinensis under heat stress at gene and protein levels. The mining of key functional genes, proteins, and pathways can also provide a basis for the cultivation of new varieties resistant to oxidative stress.

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Effects of heat shock protein 70 knockdown on the tolerance of the brine shrimp Artemia franciscana to aquaculture-related stressors: Implications for aquatic animal health and production

Cited by 8 publications

References 53 publications

Hypoxia tolerance, but not low pH tolerance, is associated with a latitudinal cline across populations of Tigriopus californicus

Hypoxia tolerance, but not low pH tolerance, is associated with a latitudinal cline across populations of Tigriopus californicus

Expression of Hsp70 in the red swamp crayfish, Procambarus clarkii in response to immune challenge

Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress

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