Effects of Hangeshashinto on the nasal physiological function: An in vitro study.

Tochigi, Kosuke; Omura, Kazuhiro; Miyashita, Keisuke; Aoki, Satoshi; Otori, Nobuyoshi; Tanaka, Yasuhiro

doi:10.1016/j.anl.2020.08.012

Cited by 2 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using HE‐stained specimens, the reconstructed sites were observed at 1 mm intervals under a × 400 high‐powered field. Eight sites were observed per sample, and they were scored using the grading system below 18,19 . The average value of the eight points was determined as the epithelialization score of one sample.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mucosal Epithelial Preservation of Free Nasal Grafts Depending on the Recipient Site

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objectives/Hypothesis Reconstruction of mucosal defects using free mucosal grafts has become a common procedure during endoscopic sinus surgery. Epithelialization of mucosal grafts affects postoperative complications and outcomes, which could be influenced by different recipient tissue. However, morphological changes occurring in the grafts transplanted over different tissues remain unexplored. Study Design An animal study. Methods Free mucoperichondrial grafts were prepared from the nasal septum of rabbits; the cartilage group had reconstruction on the nasal septal cartilage, and the perichondrium group had reconstruction on the contralateral perichondrium. The nasal septum was removed after 1 and 4 weeks of reconstruction, and the graft was histologically evaluated. Results After 1 week of reconstruction, the mucosal epithelium of grafts in the cartilage group disappeared, whereas the columnar epithelium of grafts was preserved in the perichondrium group. After 4 weeks of reconstruction, the mucosal defect site was covered with mucosal epithelium in both groups. However, while squamous epithelium was mostly observed in the cartilage group, columnar epithelium containing the healthy ciliary and goblet cells was observed in the perichondrium group. Statistically significant differences were detected in the parameters of epithelial morphology between the two groups, which were higher in the perichondrium group. Conclusions In the reconstruction of mucosal defects using free mucosal grafts, difference in recipient tissue affects the graft epithelial morphology. Level of Evidence NA Laryngoscope, 132:2301–2306, 2022

show abstract

Section: Methodsmentioning

confidence: 99%

“…Eight sites were observed per sample, and they were scored using the grading system below. 18,19 The average value of the eight points was determined as the epithelialization score of one sample.…”

Section: Evaluation Of Epithelial Morphology Of the Free Mucoperioste...mentioning

confidence: 99%

Mucosal Epithelial Preservation of Free Nasal Grafts Depending on the Recipient Site

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…An incision was made in the midline nasal skin and left lateral nasal bone to expose the left nasal septum. [23][24][25] An 8 mm-diameter punch (Kai Industries Co., Seki, Japan) was used to make an incision in the nasal septal mucosa, which was removed with forceps, and mucosal defects were created. The perichondrium was removed by scraping with a cotton ball to expose the nasal septal cartilage (Fig.…”

Section: Nasal Mucosal Damage Animal Model and The Application Of Pac...mentioning

confidence: 99%

“…The morphology of the regenerated tissue was evaluated using the epithelium grade score and subepithelial thickness. [25][26][27] Epithelium Grade Score At 1-mm intervals, eight locations in the mucosal defect area were observed with a 400Â field of view, and the morphology of the regenerated epithelium was evaluated. The epithelium grade score was determined as follows: 3, complete pseudostratified columnar epithelia with healthy cilia; 2, predominantly pseudostratified columnar epithelia with moderately healthy cilia containing non-ciliated squamous areas; 1, predominantly non-ciliated squamous epithelia or unhealthy columnar epithelia with a few or no cilia; and 0, no visible epithelium.…”

Section: Pathological Evaluation and Statistical Analysismentioning

confidence: 99%

“…The epithelium grade score was determined as follows: 3, complete pseudostratified columnar epithelia with healthy cilia; 2, predominantly pseudostratified columnar epithelia with moderately healthy cilia containing non-ciliated squamous areas; 1, predominantly non-ciliated squamous epithelia or unhealthy columnar epithelia with a few or no cilia; and 0, no visible epithelium. 25,26 The average of the eight scores was determined as the epithelium grade score of one sample.…”

Section: Pathological Evaluation and Statistical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Nasal Packing Materials and Placement Duration on Wound Healing in Nasal Mucosa: An Animal Study

et al. 2023

Self Cite

View full text Add to dashboard Cite

ObjectiveThe histological findings of wound healing depending on different nasal packing materials and replacement periods were lacking.MethodsMucosal defects were created in the nasal septum of rabbits and covered with Spongel®, Algoderm®, or Nasopore®, which were cleaned on Day 14. To investigate the effect of replacement durations, Spongel® was removed on Days 3 and 7. All nasal septal specimens were collected on Day 28. Samples without packing material were prepared as controls. Depending on residual packing materials in the regenerated tissue, specimens were classified into the remnant and non‐remnant groups, and morphology was compared using epithelium grade score and subepithelial thickness.ResultsThe epithelium grade score in the Spongel‐14d group was lower than that in the other groups (p < 0.05). Subepithelial thickness was higher in the Algoderm‐14d and Spongel‐14d groups (p < 0.05). Epithelium grade scores were higher and subepithelial thicknesses were lower in the Spongel‐3d and ‐7d groups than in the Spongel‐14d group. Epithelium grade score was lower and subepithelial thickness was higher in the remnant group (n = 10) than in the non‐remnant group (n = 15; p < 0.05).ConclusionsDifferences in packing materials and placement durations affected wound healing of nasal mucosa. The selection of appropriate packing materials and replacement duration was considered essential for ideal wound healing.Level of EvidenceNA Laryngoscope, 2023

show abstract

Effects of Hangeshashinto on the nasal physiological function: An in vitro study.

Cited by 2 publications

References 17 publications

Mucosal Epithelial Preservation of Free Nasal Grafts Depending on the Recipient Site

Mucosal Epithelial Preservation of Free Nasal Grafts Depending on the Recipient Site

Nasal Packing Materials and Placement Duration on Wound Healing in Nasal Mucosa: An Animal Study

Contact Info

Product

Resources

About