Effects of gypenoside on pancreatic beta cell function and insulin secretion

Patibandla, Chinmai; Patterson, Steven; Shu, Xinhua

doi:10.1530/endoabs.38.p279

Cited by 2 publications

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“…Gypenosides are saponins extracted from the plant Gynostemma pentaphyllum and are used as an anti-diabetic herbal tea in china. In our previous studies and various others have reported the insulin secretory properties of gypenosides in vitro and in vivo (Hoa et al, 2007;Lokman et al, 2015;Patibandla et al, 2015). We also reported the cytoprotective properties of gypenosides in BRIN-BD11 β-cells (Patibandla et al, 2015).…”

Section: Introductionsupporting

confidence: 56%

“…In our previous studies and various others have reported the insulin secretory properties of gypenosides in vitro and in vivo (Hoa et al, 2007;Lokman et al, 2015;Patibandla et al, 2015). We also reported the cytoprotective properties of gypenosides in BRIN-BD11 β-cells (Patibandla et al, 2015). Costus pictus D.Don also known as the insulin plant is used as an anti-diabetic herbal remedy in India.…”

Section: Introductionmentioning

confidence: 67%

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Cytoprotective effects of combination of gypenosides andCostus pictusD.Don extract in BRIN-BD11 β-cells

Patibandla

Campbell

Bennett

et al. 2020

Preprint

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Ethnopharmacological Relevance: Gypenosides and Costus pictus D.Don are used as an anti-diabetic herbal remedy in China and India respectively. However, the synergistic effect of these two extracts on β-cell protection is not yet elucidated. Introduction: In Type 2 diabetes mellitus (T2DM), pro-inflammatory cytokines and lipotoxicity are known causes of pancreatic β-cell dysfunction and impaired insulin secretion and eventually β-cell death. Thus, any cytoprotective drug supplements can protect the β-cell and may help in T2DM treatment. Gypenosides, extracted from the Chinese medicinal herb Gynostemma pentaphyllum and the leaf extract from an Indian medicinal herb Costus pictus D. Don are used in traditional medicine due to their insulin secretory properties. In our previous studies, both extracts have shown significant cytoprotective effects in insulin-secreting BRIN-BD11 cells. In the present study, we aim to investigate the synergistic effects of a combination of these extracts on BRIN-BD11 β-cell protection. Methods Combination of extracts was prepared by adding Gypenosides with Costus pictus at 2:1 to a concentration of 18.75mg/ml. Cell viability was determined by MTT assay following treatment with combination and/or palmitate and cytokine cocktail for 24-48h. Following 24h treatment, proliferation was measured by Ki67 staining and cytoprotective gene expression was quantified by qPCR. Results: Combination treatment of 25μg/ml enhanced cell viability both at 24h (n=8; P<0.05) and 48h (n=8; P<0.0001) treatment. Over 24h, combination treatment (25&12.5 μg/ml) showed a significant protective effect against 125μM and 250μM palmitate induced (P<0.0001) and cytokine cocktail- (TNFα 1000U, IL-1β 50U & IFNγ 1000U) (P<0.0001 & P<0.01 respectively) induced toxicity. Combination treatment over 24h increased expression of antioxidant genes Nrf2 (P<0.001), Cat (P<0.001) and Sod1 (P<0.05) along with pro-proliferative Erk1 (P<0.01) while pro-inflammatory Nfkb1 expression was reduced(P<0.001). Conclusion: The results suggest that a combination of gypenosides and costus pictus may protect β-cells against inflammatory cytokines and lipotoxicity caused by saturated free fatty acids associated with obesity and diabetes.

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