Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model

Abstract

Chronic obstructive pulmonary disease (COPD), which is characterized by airway obstruction, leads to, as the two major forms of COPD, chronic bronchitis and emphysema. This study was conducted to evaluate the effects of herbal formula, PM014, in a murine model of COPD. Balb/c mice were treated once with each herb extract in PM014 or PM014 mixture via an oral injection. Lipopolysaccharide (LPS) or elastase/LPS were administrated to the mice to induce a disease that resembles COPD. PM014 treatment significantly attenuated the increased accumulation of immune cells in bronchoalveolar lavage fluid (BALF) compared to control mice. In addition, the TNF-α and IL-6 levels in BALF were decreased in the PM014 mice. Furthermore, histological analysis demonstrated that PM014 attenuated the hazardous effects of lung inflammation. These data suggest that PM014 exerts beneficial effects against forms of COPD such as lung inflammation.

Effects of GHX02 on Chronic Obstructive Pulmonary Disease Mouse Model

“…GHX02 was formed by adding Scutellariae Radix to GRHRH. GRHRH already demonstrated its anti-inflammatory effects on COPD and particulate matter induced lung injury on a mouse model 16) and previous studies reported that Scutellariae Radix inhibits the production of several inflammatory cytokines 18) and has antioxidant effects 19) .…”

Efficacy and Safety of GHX02 in the Treatment of Acute Bronchitis and Acute Exacerbation of Chronic Bronchitis: A Phase Ⅱ, Randomized, Double-Blind, Placebo-Controlled, Multicenter Trial

“…Here, we tried to expand the effect by adding a herb ( Scutellariae radix) with a similar therapeutic mechanism. Gwaruhaengryeon-hwan demonstrated its anti-inflammatory effects on COPD and particulate matter-induced lung injury in a mouse model ( Lee et al, 2017 ). Scutellariae radix has also been reported to inhibit the production of several inflammatory cytokines ( Kim et al, 2015 ) and have antioxidant effects ( Guo et al, 2013 ).…”

Inhibitory Effects of <italic>Ssanghwa-tang</italic> on Lung Injury and Muscle Loss in a Cigarette Smoke Extract and Lipopolysaccharide-induced Chronic Obstructive Pulmonary Disease Mouse Model

“…질환이다 1 6 에 따라 살펴보면 풍한형(風寒 型)의 소청룡탕 7 , 폐열형(肺熱型)의 과루행련환 유 래 GHX02 8 , 담탁형(痰濁型)의 사간탕 유래 SGX01 9 , 폐허형(肺虛型)의 감길탕 유래 GGX 10 , 신음허형 (腎陰虛型)의 청상보하탕 유래 PM014 11 등이 있었 으나 COPD에 병발하는 근감소에 대한 영향을 동 시에 평가한 연구는 찾기 어려웠다. 쌍화탕(雙和湯)은 ≪동의보감(東醫寶鑑)≫ 12 에서 정신과 신체가 피로하고 기혈이 모두 손상되었을 때 사용하는 것으로 기재된 처방으로 사물탕(四物 湯)과 황기건중탕(黃耆建中湯)의 합방으로 구성되 어 있으며 13 , 항피로 14 , 면역활성화 15 에 대한 효능 연 구와 운동수행능력 16 , 만성피로 17 기기는 열탕추출기(Daewoong, Korea), rotary vacuum evaporator(Buchi, Switzerland), freeze dryer (EYELA, Japan), CO2 incubator(Forma Scientific, USA), micro-pipet(Gilson, France), clean bench(Vision Scientific, Korea), autoclave(Sanyo, Japan), water bath(Vision scientific, Korea), Biosystem XA(Buxco Research System, USA), vortex mixer(Vision scientific, Korea), spectrophotometer(Shimazue, Japan), thermocycler system(MWG Biotech, Germany), deep-freezer(Sanyo, Japan), centrifuge(Sigma, USA), homogenizer(OMNI, USA), plate shaker(Lab-Line, USA), ELISA reader (Molecular Devices, USA), ice-maker(Vision scientific, Korea) 및 chemical balance(Cas, Korea) 등을 사 용하였다.…”