Effects of growth regulators on callus induction and secondary metabolite production in <i>Cuminum cyminum</i>

Farvardin, Atefeh; Ebrahimi, Asa; Hosseinpour, Batul; Khosrowshahli, Mahmood

doi:10.1080/14786419.2016.1272105

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…The influence of different concentrations and combinations of growth regulators on the induction of callus from capitula of Pulicaria incisa showed a better response in the medium supplemented with 2,4D and KIN. 70% was the higher callus rate obtained onto 2.4D 4.10 -5 M and KIN 5.10 -6 M. The same observations have been noted by Gupta et al (2015) and Farvardin et al (2017) who confirmed the effectiveness of 2.4D/KIN in vitro conditions by the best induction and proliferation of callus masses in dicotyledonous plants.…”

Section: Discussionsupporting

confidence: 83%

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Rouane¹,

Chabane²,

Arab³

2019

Appl. Ecol. Env. Res.

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This study was aimed to investigate the effect of bioactive molecules of callus infusion of Pulicaria incisa (Lam.) DC, by the evaluation of anti-inflammatory activity and acute toxicity. 70% was the higher rate of callus obtained from young capitula cultivated on Murashige and Skoog (1962) (MS) solid medium containing 2.4D 4.10 -5 M and KIN 5.10 -6 M for 4 months. A Phytochemical screening based on colorimetric reactions was performed on the callus infusion material. Unlike the absence of primary components (starch, reducing sugars), secondary metabolites such as mucilage, saponins, flavonoids and anthocyanins were revealed. No sign of toxicity was observed by the application of 0.25 to 5 g/kg doses in this investigation, although, a significant anti-inflammatory response was revealed at 5 g/kg compared to that of Diclofenac ® . The chromatographic analysis (HPLC) detected the presence of 3-hydroxy-4methoxycinnamic acid the main phenolic acid produced by callus, which could be responsible of the positive anti-inflammatory reaction. These findings suggest that the callus could be an alternative source of bioactive metabolites useful in the human health as an anti-inflammatory source.

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Section: Discussionsupporting

confidence: 83%

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Rouane¹,

Chabane²,

Arab³

2019

Appl. Ecol. Env. Res.

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“…The auxins initiate cell division and control the processes of growth and cell elongation because they induce the transcription of mRNA (microRNA) encoding proteins important for growth [ 33 ]. It is often reported that 2,4-D (2,4-dichlorophenoxyacetic acid) is the most suitable auxin for callus initiation and proliferation [ 34 , 35 , 36 , 37 ]. The cytokinins regulate many cellular processes, but the control of cell division is central to the growth and development of medicinal plants [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton

Park

Seong

Kim

et al. 2020

Plants

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Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.

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“…The e cient callus induction in auxin treated medium is due to initiation of cell division, growth, and elongation; especially promoting microRNA transcription speci c to growth related protein synthesis(George et al 2008). Moreover, it is often cited that 2,4-D is a foremost suitable auxin speci c to callus induction (Anjusha and Gangaprasad 2017;Farhadi et al 2017;Farvardin et al 2017).…”

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confidence: 99%

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

Sreelek

Siril

2021

Preprint

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Dianthus chinensis L. is an edible, ornamental herb used to prepare the Dianthi Herba, a Chinese traditional rejuvenating medicine. Owing to the rapid proliferation of callus tissues, in vitro production of flavonoids has their own specific importance. Callus cultures raised followed by auxin directed biosynthesis of flavonoid through related transcript profile were carried out. Murashige and Skoog (MS) medium fortified with 2,4- Dichlorophenoxy acetic acid (2,4- D) or picloram induced formation of friable callus from internode derived cultures of D. chinensis. Culture medium containing 2,4- D (10 µM) produced the highest flavonoid content, 4.44 mg quercetin equivalent per gram (QE g− 1) under incubation in continuous dark condition, while maximum dry weight yield (0.38 g/ culture) was obtained from 10 µM 2,4- D under 16 h light / 8 h dark condition (50 µmol m− 2 s− 1 irradiance) at 60 days of incubation. The callus raised in light condition in 10 µM 2,4- D selected to analyze flavonoid related gene expression profile viz., chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3H), and flavonol synthase (FLS) at specific time intervals. The transcript abundance of CHS, F3H, or FLS gene was higher at 60 days old callus cultures and reaching 11.59, 48.31, and 114.63-fold relative expression than that of initial callus tissues respectively. These understandings are critical for the regulation of targeted phytochemicals as well as their wide exploitation in the field of biological research.

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Effects of growth regulators on callus induction and secondary metabolite production in Cuminum cyminum

Cited by 8 publications

References 18 publications

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton

In Vitro Callus Culture of Dianthus Chinensis L. for Assessment of Flavonoid Related Gene Expression Profile

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