Effects of growth factors on riboflavin production by Ashbya gossypii

Özbaş, T.; Kutsal, Tülin

doi:10.1016/0141-0229(91)90096-s

Cited by 7 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for Özbas and Kutsal (1986), they found out that optimum initial glucose concentration was 20 g/L for Eremothecium ashbyii and Ashbya gossypii. In another study, Özbas and Kutsal (1991) reported that highest riboflavin yields occurred in media with glucose and sunflower oil. Nevertheless, riboflavin production diminished when growth factors (inositol, thiamin, and biotin) were added to the medium.…”

Section: Study Of Medium Nutrients Effects Influence On Riboflavin Prmentioning

confidence: 96%

Influence of Nitrogen and Carbon Sources on Riboflavin Production by Wild Strain of Candida sp.

Suzuki

Fleuri

Macêdo

2009

Food Bioprocess Technol

View full text Add to dashboard Cite

Microorganisms are capable of producing vitamins as metabolites, which are essential nutrients, in the metabolism of energy production. So, microbial production has been researched for years. Riboflavin is one of the fewest B-vitamins biotechnologically produced. There are many factors that affect riboflavin biosynthesis, which varies in each microorganism. However, few studies of the effect of different carbons and nitrogen sources on riboflavin production have been done. Thus, our objective in this study was to work on the effect of previously selected carbon and nitrogen sources on riboflavin production by screened wild-type yeast Candida sp. LEB 130. Classical shaken fermentation vessel system was used, controlling temperature and agitation, with fixed oxygen concentration. A 2 5 screening design was used to analyze the effect of the medium components such as sucrose, flaxseed oil, and yeast extract. A second experiment factorial design 2 4 yielded a riboflavin production of 105.7 ug/mL, 4.2-fold higher than 2 5 design. Riboflavin analysis was done in a spectrophotometer. Screening design as a medium performance evaluation ended up being very advantageous for riboflavin production by this yeast.

show abstract

Section: Study Of Medium Nutrients Effects Influence On Riboflavin Prmentioning

confidence: 96%

Influence of Nitrogen and Carbon Sources on Riboflavin Production by Wild Strain of Candida sp.

Suzuki

Fleuri

Macêdo

2009

Food Bioprocess Technol

View full text Add to dashboard Cite

show abstract

“…Numerous studies have been conducted to improve riboflavin productivity by utilizing different substrates (2, 8, 9, 20). Riboflavin overproduction by A. gossypii can be achieved by using glucose, sucrose, fructose, maltose, mannose, or glycerol as the carbon sources; however, oils, such as corn oil and soybean oil, are superior (2).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production

Wei¹,

Hurley²,

Jiang³

et al. 2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.

show abstract