Effects of griseofulvin in medium‐term liver carcinogenesis assay and peripheral blood micronucleus test in rat

Labay, Karine; Ouldelhkim, Mostafa; Kles, V.; Guffroy, Magali; Poul, J; Sandérs, Pascal

doi:10.1002/tcm.1031

Cited by 11 publications

(4 citation statements)

References 38 publications

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“…Concerning aneugenic agents, colcemid and vincristine sulfate induced positive response in terms of MN induction containing a threshold (Table 3) which is in agreement with data reported for MN test in vivo (Cammerer et al 2010). Although, bisphenol A and griseofulvin have previously been shown to give a positive effect in other types of MN test in vitro (Hashimoto et al 2010;Johnson and Parry 2008), no effect in terms of MN induction have been reported here, which is in agreement with findings using MN test in vivo (Labay et al 2001;…”

Section: Discussionsupporting

confidence: 95%

A novel micronucleus in vitro assay utilizing human hematopoietic stem cells

Kotova

Hebert

Härnwall

et al. 2015

Toxicology in Vitro

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The induction of micronucleated reticulocytes in the bone marrow is a sensitive indicator of chromosomal damage. Therefore, the micronucleus assay in rodents is widely used in genotoxicity and carcinogenicity testing. A test system based on cultured human primary cells could potentially provide better prediction compared to animal tests, increasing patient safety while also implementing the 3Rs principle, i.e. replace, reduce and refine. Hereby, we describe the development of an in vitro micronucleus assay based on animal-free ex vivo culture of human red blood cells from hematopoietic stem cells. To validate the method, five clastogens with direct action, three clastogens requiring metabolic activation, four aneugenic and three non-genotoxic compounds have been tested. Also, different metabolic systems have been applied. Flow cytometry was used for detection and enumeration of micronuclei. Altogether, the results were in agreement with the published data and indicated that a sensitive and cost effective in vitro assay to assess genotoxicity with a potential to high-throughput screening has been developed.

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Section: Discussionsupporting

confidence: 95%

A novel micronucleus in vitro assay utilizing human hematopoietic stem cells

Kotova

Hebert

Härnwall

et al. 2015

Toxicology in Vitro

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“…There is some limited evidence that griseofulvin is mutagenic in the mouse lymphoma TK + /TK ) L5178Y assay (Sofuni et al, 1996). Results in the micronucleus test have generally been negative (Heddle et al, 1983;Kersten et al, 1999;Labay et al, 2001) although positive results were obtained in V79 cells and in a gut micronucleus test system (Kalweit et al, 1999;Vanhauwaert et al, 2001). Griseofulvin gave negative results in a test for DNA repair using rat and mouse hepatocytes (Mori et al, 1984) and in bacterial systems (Leifer et al, 1981).…”

Section: Griseofulvinmentioning

confidence: 99%

“…, 1983; Kersten et al. , 1999; Labay et al. , 2001) although positive results were obtained in V79 cells and in a gut micronucleus test system (Kalweit et al.…”

Section: Introductionmentioning

confidence: 99%

Veterinary pharmacovigilance. Part 6. Predictability of adverse reactions in animals from laboratory toxicology studies

Woodward¹

2005

Vet Pharm & Therapeutics

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Toxicological studies are conducted on constituents of veterinary medicinal products for a number of reasons. Aside from being a requirement of legislation, they are carried out for predictive purposes in the assessment of user safety or for the determination of consumer safety, for example, in the elaboration of maximum residue limits or tolerances. Alternatively, the results of toxicology studies may be available as they have been generated for registration of the drug for human medicinal purposes. This paper examines if the results of such studies have any predictive value for adverse reactions, which might occur during clinical use in animals. A number of adverse reactions, notably the Type A (toxicology or pharmacology dependent) should be predictable from these laboratory studies. However, as with human pharmaceutical products, they have less utility in predicting Type-B reactions (idiosyncratic in nature).

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“…The analysis of CAbs has been considered to be important because of their known role in the induction of malignancies, congenital abnormalities, and fetal wastage (Natarajan et al, 1994; Tucker and Preston, 1996) and is thus a widely used assay for testing of the mutagenic and carcinogenic effects of environmental contaminants. Another assay, the MN test system, is also widely used in genetic toxicology studies (Labay et al, 2001). MNs are small chromatin bodies that appear in the cytoplasm by the condensation of acentric chromosome fragments or by entire chromosomes (Martinez et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Fluoride and endosulfan together potentiate cytogenetic effects in Swiss albino mice bone marrow cells

2020

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In this study, the cytotoxic potential of fluoride and endosulfan in combination was investigated in Swiss albino mice bone marrow cells using the chromosomal aberration (CA) and micronucleus (MN) test systems. Fluoride (25.1 mg kg−1 body weight [bw] in water) and endosulfan (1.8 mg kg−1 bw by oral intubation) were administered orally alone and in combination (fluoride 25.1 mg kg−1 bw + endosulfan 1.8 mg kg−1 bw) to male Swiss albino mice daily for 30 days. A significant ( p < 0.01) increase in micronuclei (MNs) induction and decreased ratio ( p < 0.01) of polychromatic to normonochromatic erythrocytes (indicators of cytotoxicity) were observed compared with saline controls when animals were given the combination of fluoride and endosulfan. A significant ( p < 0.01) increase in MNs induction and no change in the polychromatic erythrocytes to erythrocyte ratio were also observed when endosulfan was given alone. CAs such as gaps, breaks, fragments, rings, exchanges, and polyploidy were recorded in the bone marrow cells. The mean percent frequency of CAs was increased ( p < 0.01) in all the treated groups compared with the control saline group. In the combination group (F + E), the percent frequencies of CAs were significantly higher (13.875%) compared with those in the individual treatment groups of fluoride (4.375%) and endosulfan (6.25%). The mitotic index was calculated as percentage of dividing cells. A significant ( p < 0.01) decrease in mitotic index was observed in all treated groups compared with controls. In the combination group (F + E), mitotic index was significantly less than ( p < 0.01; 4.1 ± 0.49) the saline control (10.8 ± 0.98). These results indicated that repeated intake of endosulfan through various sources in fluoride affected areas resulted in increased cytotoxic effects. The greater effect in the combination group indicated additive interaction of fluoride and endosulfan in inducing cytotoxicity in Swiss albino mice.

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Effects of griseofulvin in medium‐term liver carcinogenesis assay and peripheral blood micronucleus test in rat

Cited by 11 publications

References 38 publications

A novel micronucleus in vitro assay utilizing human hematopoietic stem cells

A novel micronucleus in vitro assay utilizing human hematopoietic stem cells

Veterinary pharmacovigilance. Part 6. Predictability of adverse reactions in animals from laboratory toxicology studies

Fluoride and endosulfan together potentiate cytogenetic effects in Swiss albino mice bone marrow cells

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