Effects of Green Tea Use on Wound Healing

Neves, Ana Luiza de Almeida; Komesu, Marilena Chinali; Matteo, Miguel Angel Sala Di

doi:10.4067/s0717-95022010000300039

Cited by 9 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the potential for green tea catechins to aid in epithelial formation bears further study. Although this study did not support the finding of earlier animal studies [57], other research designs may yet show a benefit in its use. In addition, in vivo angiogenesis and granulation tissue augmentation by GTE have been demonstrated [30].…”

Section: Discussioncontrasting

confidence: 99%

Dose-dependent green tea effect on decrease of inflammation in human oral gingival epithelial keratinocytes: in vitro study

et al. 2019

View full text Add to dashboard Cite

Objectives This in vitro study aimed to analyze the anti-inflammatory and wound healing potential of green tea extract (GTE) in human gingival epithelial keratinocytes (HGEK) treated with lipopolysaccharides (LPS). Materials and methods A cell viability assay was conducted using MTT to determine nontoxic levels of GTE on immortalized HGEK. Cells were concomitantly treated with LPS (1 μg/ml) and GTE (1 mg/ml, 2.5 mg/ml, 5 mg/ml, and 10 mg/ml) to assess inflammation. Gene expression levels of inflammatory markers IL-β1, IL-6, and TNFα were measured by RT-PCR and their protein production was assessed by ELISA. The scratch wound healing assay was used to investigate the effects of different concentrations of GTE on cell migration. We also explored the effect of GTE on the induction of the Nrf2/HO-1 pathway in the cells with or without LPS. Results GTE at concentrations of 2.5 mg/ml, 5 mg/ml, and 10 mg/ml significantly enhanced cell viability (p < 0.05). And IL-β1, IL-6, and TNFα gene expression presented up to 10-fold decrease compared with LPS-treated cells, which was also similarly found on the protein levels. At the same concentrations, cell migration increased. Conclusions The mechanism results showed that GTE produced the anti-inflammatory response by activating the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and increasing the level of anti-oxidant protein heme oxygenase-1 (HO-1). Clinical relevance GTE may be potentially used as oral rinse anti-inflammatory drug for treatment and prevention of oral inflammatory diseases, which is shown here by the ability to reduce the inflammation and increase in cell migration in a dosedependent manner.

show abstract

Section: Discussioncontrasting

confidence: 99%

Dose-dependent green tea effect on decrease of inflammation in human oral gingival epithelial keratinocytes: in vitro study

et al. 2019

View full text Add to dashboard Cite

show abstract

“…6 Fibroblasts are the predominant cell type in mammalian skin. Furthermore, EGCG significantly improves initial epithelial neoformation, 7 angiogenesis, and wound contraction; 8 thus, it shows promise for enhancing wound healing. However, the effectiveness of EGCG is dose dependent, and most studies recommend a dose of ,100 μM.…”

Section: Introductionmentioning

confidence: 99%

In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles

Yan

Choi

Kang

et al. 2017

IJN

View full text Add to dashboard Cite

PurposeThis study aimed to develop an anti-inflammation system consisting of epigallo-catechin-3-gallate (EGCG) encapsulated in poly(lactide-co-glycolic acid) (PLGA) particles to promote wound healing.MethodsNano- and microscale PLGA particles were fabricated using a water/oil/water emulsion solvent evaporation method. The optimal particle size was determined based on drug delivery efficiency and biocompatibility. The particles were loaded with EGCG. The anti-inflammatory effects of the particles were evaluated in an in vitro cell-based inflammation model.ResultsNano- and microscale PLGA particles were produced. The microscale particles showed better biocompatibility than the nanoscale particles. In addition, the microscale particles released ~60% of the loaded drug, while the nanoscale particles released ~50%, within 48 hours. Thus, microscale particles were selected as the carriers. The optimal EGCG working concentration was determined based on the effects on cell viability and inflammation. A high EGCG dose (100 μM) resulted in poor cell viability; therefore, a lower dose (≤50 μM) was used. Moreover, 50 μM EGCG had a greater anti-inflammatory effect than 10 μM concentration on lipopolysaccharide-induced inflammation. Therefore, 50 μM EGCG was selected as the working dose. EGCG-loaded microparticles inhibited inflammation in human dermal fibroblasts. Interestingly, the inhibitory effects persisted after replacement of the drug-loaded particle suspension solution with fresh medium.ConclusionThe EGCG-loaded microscale particles are biocompatible and exert a sustained anti-inflammatory effect.

show abstract

“…Wound healing property of Camellia sinensis, also known as green tea, has been linked to the presence of proanthocyanidins which are mainly made up of epicatechin, epigallocatechin, epicatechin-3-gallate and epigallocatechin-3-gallate and these induce differentiation of epidermal keratinocytes and also accelerate epithelial neoformation during wound healing [196].…”

Section: Flavanols and Proanthocyanidinsmentioning

confidence: 99%

Medicinal Plants and Natural Products with Demonstrated Wound Healing Properties

Agyare¹,

Bekoe²,

Boakye³

et al. 2016

Wound Healing - New Insights Into Ancient Challenges

View full text Add to dashboard Cite

This section reviews the current literature on medicinal plants including extracts, fractions, isolated compounds and natural products that have been demonstrated to have wound healing properties. Various electronic databases such as PubMed, Science Direct, SciFinder and Google Scholar were employed to search for plants, natural plant constituents and natural products that have been scientifically demonstrated to have wound healing activity using in vivo and in vitro wound models. Parameters used in the evaluation of an agent with wound healing properties include rate of wound contraction, tensile strength, antioxidant and antimicrobial activities, hydroxyproline content assay and histological investigations including re-epithelization, collagen synthesis, granulation, proliferation and differentiation of fibroblasts and keratinocytes in excision and incision wound model studies. Eighty-five medicinal plants belonging to 45 families, phytoconstituents including phenolics, oils and other substances including honey were identified as potential wound healing agents or possess wound healing properties using various wound healing models.

show abstract

Effects of Green Tea Use on Wound Healing

Cited by 9 publications

References 17 publications

Dose-dependent green tea effect on decrease of inflammation in human oral gingival epithelial keratinocytes: in vitro study

Dose-dependent green tea effect on decrease of inflammation in human oral gingival epithelial keratinocytes: in vitro study

In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles

Medicinal Plants and Natural Products with Demonstrated Wound Healing Properties

Contact Info

Product

Resources

About