Effects of green tea catechin on enzyme activities and gene expression of antioxidative system in rat liver exposed to microwaves

Kim, Miji; Choi, Jeong-Hwa; Yang, Jeong-Ah; Kim, Seong-Yong; Kim, Jung-Hye; Lee, Jun-Ha; Kim, Jongki; Rhee, Soon-Jae

doi:10.1016/s0271-5317(02)00365-2

Cited by 24 publications

(7 citation statements)

References 13 publications

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“…In accordance with our work, Kim et al [25] postulated that there was decrease in the GPx activity after exposure of the rats to microwaves for 16 days. This would seem to indicate that the peroxidation of the unsaturated fatty acid in the exisiting membranes was activated by oxidative stress, in this case free radicals resulting from microwave irradiation, thereby accelerating the cell tissue damage and decreasing the enzyme activities.…”

Section: Discussionsupporting

confidence: 93%

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Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Hewady¹,

Elkholy²,

Esmaeila³

2017

Egyptian Journal of Hospital Medicine

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Background: electromagnetic waves could cause oxidative stress and generate ROS that lead to free radical production and lipid peroxidation which damage liver tissue. Apocynin (APO) exerts its antioxidant effect by reducing ROS production via inhibition of NADPH oxidase. The present study intended to demonstrate effects of microwaves exposure on hepatic pro-oxidant/antioxidant systems and to investigate protective effects of APO against microwaves induced hepatotoxicity. Aim of the work: The aim of the present work is to evaluate the effect of Apocynin on liver toxicity induced by exposure to microwaves in rats of local strain. Subjects and Methods: Thirty local strain rats were randomly assigned into three equal groups: a) control group; b) microwave exposed group (frequency of 2.45 GHz for 15 minutes once daily for a period of 16 days); c) apocynin treated prior to microwave exposure group (apocynin 20 mg/kg intraperitoneally injected 1 hour before microwave exposure and continued during the other 16 days). Liver tissue was biochemically assessed in all groups biochemically through the determination of tissue MDA, MPO, GPx and iron in liver tissue. Also, serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and Tumor necrosis factor alpha (TNFα) levels were estimated in all groups. Results: The microwave exposed group showed that there was a significant increase in serum ALT, AST, ALP, TNF-α, as well as significant increase in the level of MPO, MDA and iron in the liver tissue. However, there was a significant decrease in the GPx and zinc level in the liver tissue. These results Were estimated and calculated in comparison with those of control group. Moreover, Apocynin treated group showed a significant decrease in serum ALT, AST, ALP, TNF-α , in addition to a significant decrease in the level of MPO, MDA and iron, along with a significant increase in the GPx level in the liver tissue, as compared with microwave exposed group. Conclusion: It is possible to conclude considering the abovementioned results that apocynin has a significantly protective effect against hepatotoxicity induced by microwaves exposure.

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Section: Discussionsupporting

confidence: 93%

“…The animals of this group were exposed to microwaves at frequency of 2.45 GHz for 15 minutes once daily for a period of 16 days [7] .…”

Section: -Microwave Exposed Groupmentioning

confidence: 99%

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Hewady¹,

Elkholy²,

Esmaeila³

2017

Egyptian Journal of Hospital Medicine

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“…Antioxidant enzymes play an important role in cellular protection against oxidative stress‐induced cell damage (Sun et al., 2005; Kang et al., 2006). The mechanisms of regulation of antioxidant enzyme activity and mRNA expression by ROS or TC have been reported (Kim et al., 2002; Na and Surh, 2008). The predominant enzymes in enzymatic defence systems against H 2 O 2 are CAT and GPx, and the activity of them increased with additional of H 2 O 2 exposure (Pietarinen et al., 1995; Tate et al., 1995; Röhrdanz and Kahl, 1998).…”

Section: Discussionmentioning

confidence: 99%

Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H₂O₂‐induced primary hepatocytes of goat in vitro

Zhong

Xiao

Zhou

et al. 2012

Animal Physiology Nutrition

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Tea catechins (TC) are polyphenols that have potent antioxidant activity. The objectives of this study were to determine the effects of TC on antioxidant status of hepatocytes challenged with H2 O2 . Primary hepatocytes of goat were exposed to 1 mm H2 O2 without or with 5, 50 and 500 μg/ml TC. The cells were harvested at 48 h post-treatment to determine effects of TC on proliferation, apoptotic features and membrane integrity of cells, and expression of genes and activities of antioxidant enzymes. H2 O2 exposure caused damage to cells (p < 0.001). A lower concentration of TC (5 μg/ml) displayed a protective effect by inhibiting exorbitant cell proliferation and DNA degradation. Both H2 O2 exposure and TC pre-incubation affected expression of antioxidant enzymes at mRNA and protein levels (p < 0.001). The activities of catalase (CAT) (p = 0.027), CuZn-superoxide dismutase (CuZn-SOD) (p < 0.001) and glutathione peroxidase (GPx) (p < 0.001) increased with TC pre-incubation followed by H2 O2 challenge. Changes of CuZn-SOD activity induced by H2 O2 and TC basically paralleled the changes in the corresponding mRNA and protein levels, but the correlation in CAT and GPx expression displayed slightly different patterns at different concentrations of TC. These findings infer that oxidative stress can induce deleterious cellular responses and this unfavourable condition may be alleviated by treatment with TC.

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“…The flavonoids in green tea have been shown to be efficient scavengers of reactive oxygen species, and green tea blocks the in vivo production of 4‐OH‐nonenal, a product of lipid peroxidation 44, 59. Green tea powder was recently shown to induce hepatic gene expression of antioxidant enzymes such as superoxide dismutase, GSH, and peroxidase in rats exposed to microwave‐induced oxidative stress 60. GSH levels decrease in the presence of reactive oxygen species as a mechanism of protection against cellular injury 31.…”

Section: Discussionmentioning

confidence: 99%

Short-term administration of (-)-epigallocatechin gallate reduces hepatic steatosis and protects against warm hepatic ischemia/reperfusion injury in steatotic mice

et al. 2005

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Effects of green tea catechin on enzyme activities and gene expression of antioxidative system in rat liver exposed to microwaves

Cited by 24 publications

References 13 publications

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H₂O₂‐induced primary hepatocytes of goat in vitro

Short-term administration of (-)-epigallocatechin gallate reduces hepatic steatosis and protects against warm hepatic ischemia/reperfusion injury in steatotic mice

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Effects of green tea catechin on enzyme activities and gene expression of antioxidative system in rat liver exposed to microwaves

Cited by 24 publications

References 13 publications

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Effect of Apocynin on Liver Toxicity Induced by Microwaves in Rats

Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H2O2‐induced primary hepatocytes of goat in vitro

Short-term administration of (-)-epigallocatechin gallate reduces hepatic steatosis and protects against warm hepatic ischemia/reperfusion injury in steatotic mice

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Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H₂O₂‐induced primary hepatocytes of goat in vitro