Effects of green LED light and three stresses on biomass and lipid accumulation with two-phase culture of microalgae

Improving Fucoxanthin Production in Mixotrophic Culture of Marine Diatom Phaeodactylum tricornutum by LED Light Shift and Nitrogen Supplementation

“…It is noteworthy that the cell density was increased rapidly in GL + T in phase 2 ( Figure 4C ), but the lowest biomass concentration was observed ( Figure 4D ). Similarly in the previous report, the biomass entered stable phase and the lipid content increased by 53% and 29% when light shifted from R:B (50:50) ( Sirisuk et al, 2018 ) and BL ( Jung et al, 2019 ) to green light, respectively. Additionally, the content and intercellular amount of Fx were the lowest in GL + T compared to other groups, resulting in the lowest volumetric concentration of Fx ( Figure 4D and Table 4 ).…”

“…Compared to the control group (BL + T) in phase 2, even though the Fx content was lower in R:B (5:1) + T group (p < 0.05), the intercellular amount of Fx was similar in the two groups (Table 4) but the volumetric concentration of Fx (84.48 mg L −1 ) achieved the highest level (p < 0.05) ( Figure 4D). Therefore, the shift to R: B (5:1) with previous report, the biomass entered stable phase and the lipid content increased by 53% and 29% when light shifted from R:B (50:50) (Sirisuk et al, 2018) and BL (Jung et al, 2019) to green light, respectively. Additionally, the content and intercellular amount of Fx were the lowest in GL + T compared to other groups, resulting in the lowest volumetric concentration of Fx (Figure 4D and Table 4).…”

“…Additionally, the content and intercellular amount of Fx were the lowest in GL + T compared to other groups, resulting in the lowest volumetric concentration of Fx (Figure 4D and Table 4). It was reported that carotenoids content was reduced after 3-days green light exposure (Jung et al, 2019), and the genes encoding LCYB, ZEP1, and ZEP 2 performed high initial transcription levels, then balanced out the difference after 24 h exposure to green light (Valle et al, 2014), which explained the reduction of Fx under green light. Additionally, the previous study indicated that Chl a was more inhibited compared to carotenoids under green light induction (Jung et al, 2019).…”

“…For example, a hetero-photoautotrophic two-phase cultivation with white: blue light (1:1) induction was used in marine diatom Nitzschia laevis for Fx accumulation ( Lu et al, 2018 ). Two-phase culture using different light regimes has also applied in inducing lipid accumulation in P. tricornutum ( Sirisuk et al, 2018 ; Jung et al, 2019 ). It is noteworthy that light is not only energy source in the mixotrophic culture of microalgae, but also a role of inducing factor in accumulation of biomass and bioactive compounds in diatom.…”

Effects of Monochromatic Illumination with LEDs Lights on the Growth and Photosynthetic Performance of Auxenochlorella protothecoides in Photo- and Mixotrophic Conditions

“…The effects on the P L cultures confirm this hypothesis. A few other reports confirm that microalgae do not grow well under green light [25][26][27] because this wavelength is poorly absorbed by the photosynthetic antenna and cannot provide useful energy for photosynthesis. However, there is disagreement concerning yellow light, as Hultberg et al [26] and de Mooij et al [28] found that yellow light was very effective for biomass production of Chlorella vulgaris and Chlamydomonas reinhardtii, respectively.…”