Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derived from the goat ovary

Qiu, Mingning; Quan, Fusheng; Han, Chengquan; Wu, Bin; Li, Jun; Yang, Zhongcai; Zhang, Yong

doi:10.1016/j.jsbmb.2013.06.005

Cited by 34 publications

(25 citation statements)

References 34 publications

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“…Compared with those of theca cells, cortical stromal cells exhibit lower levels of progesterone and androstenedione secretion as well as lower LHR expression in cattle. These results are consistent with those in cattle and goats from previous studies (Orisaka et al, ; Parrott & Skinner, ; Qiu et al, ). Next, we used an RNAi method to investigate the effect of the downregulation of WT1 on cortical stromal cell differentiation.…”

Section: Discussionsupporting

confidence: 93%

“…However, in this study, the downregulation of WT1 in cortical stromal cells did not affect the expression of LHR or the LH‐induced androstenedione secretion. Previous studies have shown that the increase in LHR expression may be related to insulin growth factor 1 and to the factors secreted by granulosa cells (Orisaka et al, ; Qiu et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…An increased LH response has been associated with the differentiation of cortical stromal cells into theca cells (Orisaka et al, ; Qiu et al, ). Therefore, in the present study, we investigated the effect of WT1 downregulation on the LH response in cortical stromal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Adult bovine ovaries were collected from a local abattoir in Xi’An and were isolated according to the established laboratory standards of Northwest A&F University, China. The cortical stroma and theca layer were dissociated from the ovaries as previously described with minor modifications (Orisaka et al, ; Qiu et al, ). Briefly, the ovarian surface epithelium was removed using surgical forceps, and the cortical stroma was cut into thin strips.…”

Section: Methodsmentioning

confidence: 99%

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Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Meng

Wang

et al. 2019

Molecular Reproduction Devel

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Bovine theca cells are thought to differentiate from cortical stromal cells, and ovary‐derived Wilms’ tumor 1+ (WT1+) cells are the primary source of mouse theca cells. However, it is not known whether the differentiation of cortical stromal cells is regulated by WT1. Here, we identified WT1 in the cortical stroma and theca layer of the bovine ovary and analyzed the theca cell functional markers in cortical stromal cells and theca cells; in addition, we determined the effects of this gene on the secretion of androstenedione and progesterone by cortical stromal cells and the responsiveness of cortical stromal cells to luteinizing hormone (LH) in vitro. We used quantitative reverse‐transcription polymerase chain reaction (RT‐qPCR), western blot analysis, and immunohistochemistry to discover that the cortical stroma had higher WT1 expression than the theca layer. We used RT‐qPCR and ELISA analyses to determine that the cortical stromal cells had lower levels of androstenedione and progesterone secretion and LHR messenger RNA expression than the levels of the theca cells. In cultured bovine cortical stromal cells, we found that WT1 downregulation increased androstenedione and progesterone secretion but had no effect on the LH responsiveness. Notably, the increase in androstenedione and progesterone secretion was associated with an increase in 3‐β‐hydroxysteroid dehydrogenase expression. In conclusion, the results suggest that WT1 is involved in the differentiation of cortical stromal cells into cells with characteristics similar to theca cells of antral follicles in adult bovine ovaries.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Meng

Wang

et al. 2019

Molecular Reproduction Devel

Self Cite

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“…These reactions were repeated in triplicate for every sample as technical replicates. Gene mRNA quantifications were performed using the 2 −△△Ct method, and the amount of transcripts in each sample was normalized using GAPDH as the internal control gene to correct for differences in the cDNA used (Qiu et al 2013). …”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Wang

Lin

Yin

et al. 2015

Cell Stress and Chaperones

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Brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. Brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease. In this study, we describe the Brucella suis vaccine strain S2 (B.suis.S2) infection and replication in the immortalized caprine endometrial epithelial cell line hTERT-EECs and the induced cellular and molecular response modulation in vitro. We found that B.suis S2 was able to infect and replicate to high titers and inhibit the proliferation of EECs and induce non-apoptotic pathways, as determined by B.suis.S2 detection using MTT and acridine orange/ethidium bromide (AO/EB) staining and flow cytometry. We explored the evidence of non-apoptotic pathways using real-time quantitative RT-PCR and by western blot analysis. Finally, we discovered the over-expression of GRP78, ATF4, ATF6, PERK, eIF2α, CHOP, and cytochrome c (Cyt-c) but not IRE1, xbp-1, and caspase-3 in B.suis.S2 (HK)-attacked and B.suis.S2-infected cells, suggesting that the molecular mechanism of ER stress sensor activation by B.suis.S2 is basically concomitant with that by B.suis.S2 (HK) and that ER stress, especially the PERK pathway, plays an important role in the process of B.suis.S2 infecting EEC, which may, in part, explain the role of the uterus in the pathogenesis of B.suis.S2.

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Transplantation of Isolated Follicles and the Engineered Ovary

Amorim

Asiabi

Ouni

et al. 2022

Female and Male Fertility Preservation

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Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derived from the goat ovary

Cited by 34 publications

References 34 publications

Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Evidence that downregulation of Wilms’ tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Transplantation of Isolated Follicles and the Engineered Ovary

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