Effects of Granulocyte-Colony Stimulating Factor on the Polymorphonuclear Leukocyte Activity and the Course of Sepsis in Rats with Experimental Peritonitis

Gurleyik, Gunay; Yanikkaya, Gulderen; Gürleyik, Emin; Öztürk, Erol; Dulundu, Ender; Sağlam, Abdullah

doi:10.1007/s00595-005-3399-3

Cited by 4 publications

(8 citation statements)

References 18 publications

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“…Furthermore, both G-CSF and GM-CSF are involved in enhanced leukocyte accumulation in the perivascular tissue of growing collaterals [7,14,25], a key step in arteriogenesis [11,12,26]. Several studies showed that G-CSF not only influences granulocyte development and function, but also augments monocyte migration and activation in the perivascular tissue of the growing collaterals [27,28,29]. In contrast to Sugiyama et al [22], we could demonstrate that monocyte migration towards an MCP-1 gradient can be stimulated by GM-CSF, G-CSF and M-CSF alike.…”

Section: Discussionmentioning

confidence: 99%

Granulocyte Colony-Stimulating Factor Improves Cerebrovascular Reserve Capacity by Enhancing Collateral Growth in the Circle of Willis

Duelsner¹,

Gatzke²,

Gläser³

et al. 2012

Cerebrovasc Dis

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Background and Purpose: Restoration of cerebrovascular reserve capacity (CVRC) depends on the recruitment and positive outward remodeling of preexistent collaterals (arteriogenesis). With this study, we provide functional evidence that granulocyte colony-stimulating factor (G-CSF) augments therapeutic arteriogenesis in two animal models of cerebral hypoperfusion. We identified an effective dosing regimen that improved CVRC and stimulated collateral growth, thereby improving the outcome after experimentally induced stroke. Methods: We used two established animal models of (a) cerebral hypoperfusion (mouse, common carotid artery ligation) and (b) cerebral arteriogenesis (rat, 3-vessel occlusion). Following therapeutic dose determination, both models received either G-CSF, 40 µg/kg every other day, or vehicle for 1 week. Collateral vessel diameters were measured following latex angiography. Cerebrovascular reserve capacities were assessed after acetazolamide stimulation. Mice with left common carotid artery occlusion (CCAO) were additionally subjected to middle cerebral artery occlusion, and stroke volumes were assessed after triphenyltetrazolium chloride staining. Given the vital role of monocytes in arteriogenesis, we assessed (a) the influence of G-CSF on monocyte migration in vitro and (b) monocyte counts in the adventitial tissues of the growing collaterals in vivo. Results: CVRC was impaired in both animal models 1 week after induction of hypoperfusion. While G-CSF, 40 µg/kg every other day, significantly augmented cerebral arteriogenesis in the rat model, 50 or 150 µg/kg every day did not show any noticeable therapeutic impact. G-CSF restored CVRC in mice (5 ± 2 to 12 ± 6%) and rats (3 ± 4 to 19 ± 12%). Vessel diameters changed accordingly: in rats, the diameters of posterior cerebral arteries (ipsilateral: 209 ± 7–271 ± 57 µm; contralateral: 208 ± 11–252 ± 28 µm) and in mice the diameter of anterior cerebral arteries (185 ± 15–222 ± 12 µm) significantly increased in the G-CSF groups compared to controls. Stroke volume in mice (10 ± 2%) was diminished following CCAO (7 ± 4%) and G-CSF treatment (4 ± 2%). G-CSF significantly increased monocyte migration in vitro and perivascular monocyte numbers in vivo. Conclusion: G-CSF augments cerebral collateral artery growth, increases CVRC and protects from experimentally induced ischemic stroke. When comparing three different dosing regimens, a relatively low dosage of G-CSF was most effective, indicating that the common side effects of this cytokine might be significantly reduced or possibly even avoided in this indication.

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Section: Discussionmentioning

confidence: 99%

Granulocyte Colony-Stimulating Factor Improves Cerebrovascular Reserve Capacity by Enhancing Collateral Growth in the Circle of Willis

Duelsner¹,

Gatzke²,

Gläser³

et al. 2012

Cerebrovasc Dis

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“…Nine articles analyzed neutrophil migration focusing on evaluating the presence or absence of molecules involved in the direct modification of neutrophil migration capacity or treatment and analysis of soluble plasma molecules indirectly related to neutrophil migration. All studies detected a reduction in migratory capacity in cells derived from animal experimental models of sepsis or patients with sepsis ( 11 – 15 ).…”

Section: Resultsmentioning

confidence: 96%

“…In molecular analyses, the role of granulocyte colony-stimulating factor (G-CSF) was highlighted, showing the reduction of neutrophil migration as well as improvement in the immune response of rats subjected to peritonitis and treated with this factor ( 11 ). G-CSF was therefore associated with improvement in the immune response of neutrophils in severe infection ( 11 ).…”

Section: Resultsmentioning

confidence: 99%

Neutrophil activity in sepsis: a systematic review

Resende

Borges

Gonçalves

et al. 2020

Braz J Med Biol Res

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The neutrophil is an important cell in host defense against infections, acting as the first line of microorganism control. However, this cell exhibits dysregulated activity in sepsis and may contribute to the pathogenesis of the disease. This systematic review aimed to highlight the major scientific findings regarding neutrophil activity in sepsis reported in clinical and experimental research published in the last 10 years. The search was conducted in the Virtual Health Library of PAHO-WHO (BVS) and PubMed databases, and articles published between January 2007 and May 2017 in Portuguese, English, and Spanish were eligible. Article selection was carried out independently by two reviewers (CB and IB). A total of 233 articles were found, of which 87 were identified on PubMed and 146 on BVS. Eighty-two articles were duplicates. Of the remaining 151 articles, 19 met the inclusion criteria after title, abstract, and full-text analysis. Overall, research in clinical samples and animal models of sepsis showed reduced capacity of neutrophils to migrate and delayed apoptosis, but there was no consensus on the phagocytic activity of neutrophils in sepsis. Molecules, such as pentraxin 3 (PTX3), have been analyzed as potential diagnostic markers in sepsis but the diversity of soluble molecules detected in blood samples of sepsis patients did not enable further understanding of the correlation of these circulating molecules with neutrophil activity during sepsis. Optimal understanding of the function of neutrophils in sepsis remains a challenge that, if overcome, would eventually allow targeted therapeutic interventions in patients affected by this severe syndrome.

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“…The peritoneal irrigation with ESAW in experimental peritonitis created by a cecal puncture also proved to be as effective as granulocyte-colony stimulation factor (G-CSF). 23 Gurleyik et al 23 demonstrated that the subcutaneous injection of 50 mg/kg G-CSF increased the 4-day survival rate from 20% to 90%. Such rates are very close to those observed in the present study, from 25% to 85%.…”

Section: Discussionmentioning

confidence: 98%

Effect of electrolyzed strong acid water on peritoneal irrigation of experimental perforated peritonitis

et al. 2009

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Effects of Granulocyte-Colony Stimulating Factor on the Polymorphonuclear Leukocyte Activity and the Course of Sepsis in Rats with Experimental Peritonitis

Cited by 4 publications

References 18 publications

Granulocyte Colony-Stimulating Factor Improves Cerebrovascular Reserve Capacity by Enhancing Collateral Growth in the Circle of Willis

Granulocyte Colony-Stimulating Factor Improves Cerebrovascular Reserve Capacity by Enhancing Collateral Growth in the Circle of Willis

Neutrophil activity in sepsis: a systematic review

Effect of electrolyzed strong acid water on peritoneal irrigation of experimental perforated peritonitis

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